hexon (AC_000017) Virus Tagged ORF Clone

CAT#: VC100015

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus 1], codon optimized for human cell expression, AP_000512



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CNY 14,540.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100015 represents NCBI reference of AP_000512 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACTCCTTCCATGATGCCACAATGGTCTTATATGCACATCTCCGGACAGGATGCATCTGAGTATT
TGTCCCCAGGACTGGTGCAGTTCGCCAGGGCCACAGAGACATATTTCTCTCTGAACAATAAGTTCAGGAA
CCCTACCGTGGCTCCTACCCACGATGTGACTACCGACCGATCTCAGAGACTCACCTTGCGCTTTATTCCT
GTGGATCGAGAGGACACCGCGTACTCCTACAAAGCCCGCTTTACTTTGGCAGTAGGCGACAATAGAGTTC
TGGATATGGCCAGTACATATTTTGATATTAGAGGCGTCCTGGACCGCGGACCTACCTTCAAGCCTTACAG
CGGCACAGCCTATAACGCGCTGGCCCCTAAAGGCGCACCTAACTCCTGCGAGTGGGAGCAGGAGGAGCCA
ACACAGGAAATGGCAGAGGAGCTCGAGGACGAAGAGGAGGCAGAAGAAGAGGAGGCTGAGGAGGAGGCCG
AAGCGCCCCAGGCCGACCAGAAGGTCAAGAAAACCCATGTATACGCACAGGCGCCCCTGGCCGGAGAGAA
GATTACGGCCAATGGGCTGCAGATAGTCTCCGACACCCAGACCGAAGGAAATCCCGTGTTCGCCGATCCG
ACTTATCAGCCTGAGCCGCAGGTTGGTGAAAGCCAGTGGAATGAAGCCGAAGCTACAGCCAGTGGGGGCC
GCGTCTTGAAGAAGACAACTCCGATGAAGCCTTGCTACGGTTCCTACGCCCGGCCAACAAACAAGAATGG
AGGCCAAGGTATCCTGGTGGCCAACAACCAAGGCGCTCTGGAGAGCAAAGTGGAGATGCAATTCTTTGCC
CCGTCCGGCACTGCTATGAACGAAAGAAACGCCGTACAGCCCAGCATTGTTCTCTATAGCGAAGACGTAA
ATATGGAGACACCTGATACACACATCTCTTACAAGCCCTCAAAAACCGATGAAAACTCCAAGGCCATGCT
GGGGCAACAGGCAATGCCTAATAGACCCAACTACATCGCCTTTAGAGACAACTTCATCGGGCTCATGTAT
TACAATTCCACCGGTAACATGGGGGTGCTCGCTGGACAAGCATCCCAACTGAACGCAGTTGTTGATCTTC
AGGACAGAAACACAGAGCTGTCCTATCAGCTGCTGCTTGATTCCATCGGCGATCGGACACGCTATTTCTC
CATGTGGAATCAAGCAGTGGATAGTTATGACCCAGATGTGAGAATCATCGAAAATCACGGAACGGAGGAT
GAACTGCCTAACTACTGCTTCCCACTGGGCGGAATCGGGGTTACCGACACCTATCAGGGGATTAAGAGTA
ATGGTAATGGGAACCCCCAGAATTGGACCAAAAACGACGACTTCGCAGCACGCAACGAGATTGGTGTCGG
CAATAACTTCGCCCTCGAAATCAATCTGAACGCCAACCTGTGGCGCAATTTTTTGTATAGCAACATCGCC
CTGTACCTTCCTGACAAGCTTAAATATACGCCAACGAACGTAGAAATAAGCCCCAATCCAAATTCATATG
ACTATATGAACAAGAGGGTGGTGGCACCCGGTCTCGTGGATTGCTACATCAATCTGGGTGCTAGGTGGAG
CCTTGACTATATGGATAATGTCAACCCTTTCAACCACCATCGAAATGCCGGCCTCAGATATAGATCTATG
CTGCTGGGCAACGGCCGCTATGTTCCATTCCATATACAGGTGCCTCAGAAGTTCTTTGCCATCAAGAACC
TCCTGCTGCTGCCAGGTTCATACACCTATGAGTGGAATTTCAGGAAGGACGTTAACATGGTCCTCCAATC
CAGCTTGGGCAACGACCTGAGGGTGGATGGGGCGTCTATCAAGTTCGATAGCATTTGTCTGTACGCAACT
TTCTTCCCTATGGCCCACAATACTGCCAGCACCCTCGAGGCTATGCTGAGGAATGATACAAATGATCAGT
CCTTTAATGATTATTTGTCCGCCGCCAATATGCTCTATCCAATTCCTGCTAATGCCACAAATGTCCCTAT
CTCCATACCAAGTCGAAACTGGGCCGCTTTTCGCGGGTGGGCTTTCACTCGCCTGAAGACCAAAGAGACC
CCATCCCTGGGCTCTGGGTATGATCCCTATTACACATATTCAGGGTCCATTCCTTACCTGGACGGCACTT
TCTACCTGAACCACACATTTAAGAAGGTAGCCATAACCTTCGACAGCAGCGTGTCCTGGCCTGGCAACGA
CCGACTGCTTACCCCCAATGAATTCGAAATTAAGAGGTCCGTGGATGGCGAGGGATACAATGTTGCTCAG
TGCAATATGACTAAAGACTGGTTCCTGGTCCAAATGCTGGCTAACTACAATATCGGCTACCAAGGGTTCT
ATATCCCTGAATCCTACAAAGATCGCATGTACAGCTTCTTTCGAAACTTTCAGCCCATGTCTCGACAGGT
TGTGGACGATACTAAATATAAAGATTACCAGCAGGTCGGTATTCTGCATCAGCATAATAACAGCGGGTTT
GTGGGATATCTCGCCCCAACTATGCGGGAAGGCCAGGCCTACCCCGCTAATTTCCCTTATCCTCTTATCG
GCAAGACCGCTGTCGATAGCATAACTCAGAAGAAGTTCCTGTGCGACAGAACACTCTGGCGCATCCCATT
TTCCTCCAACTTTATGAGCATGGGGGCTCTCACTGACTTGGGACAGAACCTGTTGTATGCCAACAGCGCT
CACGCACTGGACATGACTTTCGAGGTGGACCCGATGGATGAGCCCACCCTCCTGTATGTGCTGTTTGAAG
TGTTTGATGTGGTGCGCGTCCATCAGCCTCATCGCGGCGTGATAGAGACGGTGTACCTGAGGACCCCATT
CTCTGCAGGAAACGCCACCACT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100015 representing AP_000512
Red=Cloning sites Green=Tags

MATPSMMPQWSYMHISGQDASEYLSPGLVQFARATETYFSLNNKFRNPTVAPTHDVTTDRSQRLTLRFIP
VDREDTAYSYKARFTLAVGDNRVLDMASTYFDIRGVLDRGPTFKPYSGTAYNALAPKGAPNSCEWEQEEP
TQEMAEELEDEEEAEEEEAEEEAEAPQADQKVKKTHVYAQAPLAGEKITANGLQIVSDTQTEGNPVFADP
TYQPEPQVGESQWNEAEATASGGRVLKKTTPMKPCYGSYARPTNKNGGQGILVANNQGALESKVEMQFFA
PSGTAMNERNAVQPSIVLYSEDVNMETPDTHISYKPSKTDENSKAMLGQQAMPNRPNYIAFRDNFIGLMY
YNSTGNMGVLAGQASQLNAVVDLQDRNTELSYQLLLDSIGDRTRYFSMWNQAVDSYDPDVRIIENHGTED
ELPNYCFPLGGIGVTDTYQGIKSNGNGNPQNWTKNDDFAARNEIGVGNNFALEINLNANLWRNFLYSNIA
LYLPDKLKYTPTNVEISPNPNSYDYMNKRVVAPGLVDCYINLGARWSLDYMDNVNPFNHHRNAGLRYRSM
LLGNGRYVPFHIQVPQKFFAIKNLLLLPGSYTYEWNFRKDVNMVLQSSLGNDLRVDGASIKFDSICLYAT
FFPMAHNTASTLEAMLRNDTNDQSFNDYLSAANMLYPIPANATNVPISIPSRNWAAFRGWAFTRLKTKET
PSLGSGYDPYYTYSGSIPYLDGTFYLNHTFKKVAITFDSSVSWPGNDRLLTPNEFEIKRSVDGEGYNVAQ
CNMTKDWFLVQMLANYNIGYQGFYIPESYKDRMYSFFRNFQPMSRQVVDDTKYKDYQQVGILHQHNNSGF
VGYLAPTMREGQAYPANFPYPLIGKTAVDSITQKKFLCDRTLWRIPFSSNFMSMGALTDLGQNLLYANSA
HALDMTFEVDPMDEPTLLYVLFEVFDVVRVHQPHRGVIETVYLRTPFSAGNATT

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN AC_000017
ORF Size 2892 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AC_000017.1, AP_000512
RefSeq ORF 2892 bp
MW 108.8 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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