pol (AC_000005) Virus Tagged ORF Clone

CAT＃: VC100224

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for pol [Human adenovirus A], codon optimized for human cell expression, AP_000112

Datasheet

SDS

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CNY 17,860.00

货期*

7周

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

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CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

TA592569

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 600.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA50011-100

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

CNY 1,050.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC100224 represents NCBI reference of AP_000112 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATTGGTTCAAAATAACCGCGCCGGGTCTGTCTACACTACACCAACTGACTCAAGATATCAGTCTC CAGGGAGTCGGCCTTGTCAGTCCACTAGCGGTTCTACACCCTCACCAGCCCGAGCCTCCGGTCGGCATAC CGCCTCACCCGCTAGTCGGAGGACGAGAACTGCCGCATCTAGTGGCTCTTCTTCAGCACCACCAATCCTG AGCCGGCGAATCGGGAAGGTGCACCGGGGGACCGTGGTGTCACCCAGAGCGCATGGAATCCTGCACGCCA TCGACAGCGCCAACGGTACACCCCTGGAGATCAAGTACTACTTGCACCTCCAACCAGCATTGACGCGCCT GTGTGAGGTGAATCTGAGGGCCGTGCCAGCTGATATGTTGTTTTCACTCACTGATACTATGGACAGCAGC AGGCTGTACGCTCTGATCTCCCGATTCAGGCCCAGTAGAGCTGAGATCTGGACATGTCGCAGCAGGGGGA CGGTTACCCTCAGCGTCCTCGCCTTCGAGGACCCCAATGGAAGCGGGAATGCCGCCGAGGAGGAAGAGGA CGAGCGACAGCTGCCTAGCGGGATCGACTTTCCTATTTGCTTCCTGGTACGGGGAAGACAAGTGCACCTT ATCCAGCAAATTCAGCCTGTGCAGCGGTGCGAGCACTGCGCACGGTTCTATAAGCATCAGCACGAGTGCT CAGTCAGAAGACGGGACTTCTACTTCCATCATATCAATTCTCACAGCAGCAACTGGTGGCAGGAAATTCA CTTCTTTCCAATCGGAAGCCACCCCAGAACGGAGCGCCTGTTCATTACCTACGACGTCGAGACATATACG TGGATGGGCGCCTTTGGTAAACAGCTCGTCCCATTCATGCTGGTTATGAAGCTCTCCGGAGACGATAACT TGGTTAAACACGCTCTCCAGCTGGCGCTCGAGTTGGGATGGGACCAGTGGGAAAAGGATTCCACCACATT TTACTGCCTGACCCCGGAAAAGATGAAGGTCGGCCAACAGTTCCGCACATACCGCAATCGGCTGCAGACT TCTCTCGCCACCGATTTGTGGATGACGTTTCTTCAAAAGAATCCGCATCTTTCTCAGTGGGCACAGGAGG AGAATGGTCTGGTTGCCCTGGAGGACCTCTCTTACGAAGACTTGAAGCGGGCACCCGCTATTAAGGGCGA ACCGCGGTTTGTAGAACTGTATATCGTTGGACATAATATCAATGGGTTCGACGAGATCGTTTTGGCAGCC CAGGTCATTAATAACAGACTCGATGTCCCTGGACCCTTCAAAATTAGCCGGAATTTCATCCCTCGGGCCG GGAAAATCCTGTTCAACGACATTACATTTGCGCTTCCGAACCCACATTACAAGAAGCGCACAAATTTTCT CCTGTGGGAGCACGGCGGCTGCGATGACCAAGATTTCAAGTATCAGTACCTGAAAGTCATGGTGCGCGAC ACTTTTGCCCTGACCCACACTTCCCTCAGGAAAGCTGCACAGGCCTACGCTCTGCCCGTGGAAAAAGGAT GCTGCCCATACAAAGCTGTGAATCAGTTTTACATGCTGGGTTCATATCGCGCCGACGCTAACGGTTTCCC CCTTGAGGAGTATTGGAAGGATAAGGAAGAATACCTGCTGAATCAAGAACTGTGGAAAAAGAAGGGCGAG AAGAATTACAATCTCATCGGAGAGACTTTGAATTATTGCGCTCTGGACGTTCTGGTCACCGCAAGCTTGG TCGAAAAGCTGCGCTCCTCCTACGCTCAGTTCGTGACGGATGCAGTCGGGCTCGATGCAGCCCATTTTAA TGTGTTTCAGCGACCCACTATCAGTAGCAACTCTCATGCCATATTCAGGCAAATTCTGTACAGAGCAGAG AAGCCGCAGAGGACCCACCTCGGCCCCAACATCCTGGCCCCCTCCCATGAGCTGTATGACTACGTGAGAG CAAGTATACGAGGTGGGCGGTGTTACCCTACATACATTGGTGTGCTGAAAGAACCCATATACGTGTATGA CATTTGTGGCATGTACGCGAGCGCCCTGACTCATCCCATGCCTTGGGGACCTCCACTGAATCCGTATGAG CGAGCGCTCGCCGTCAGGCAGTGGCAGGTGGCACTTGAGAATTACACTTGCAAGATCGACTATTTTGACA AGAATCTGTGCCCTGGCATCTTCACCATCGATGCTGATCCTCCTGATGAAAACCAGCTGGACGTCCTTCC GCCATTCTGCTCAAGGAAAGGCGGTAGGCTGGCCTGGACAAATGAAAGTCTGAGGGGAGAGGTTGTGACG AGCGTGGACTTGGTTACGCTGCATAATAGGGGGTGGCGCCTCAGACTGCTGTCAGACGAAAGAACCACCA TCTTCCCTACTTGGAAGTGTTTGGCCCGGGAGTACGTGCAGCTTAATATCGCAGCCAAGGAACGGGCCGA CAGGGACAAGAACCAAACTCTGAGGTCTATCGCAAAACTGCTTAGCAACGCTCTTTATGGATCCTTCGCT ACCAAACTTGACAATAAGAAAATAGTATTCAGCGATCAGATGGAGGAGAGCCTTATGAAGGAGATCGCTG CAGGCCGGCTGAACATTAAATCTTCCTCTTTCATAGAGACGGATACGCTGAGTACTGAGGTCATGCCCGC TTTCGAGAGGGTCTACAGCCCAAACCAGCTGGCCCTGGTGAATTCAGAGGCCGAAGAAAGCGATGAGGAC CAAGGTCCCGCCCCCTTCTACAGTCCACCTCCCGAAAACTGTGAACACGTGACTTACACCTATAAACCTA TCACTTTTATGGACGCTGAGGAAGGCGATATGTGTCTCCATACTCTGGAGTCATCCAATCCTCTGATTAA TAATGACAGATACCCAAGCCATGTGGCATCATTCGTACTCGCTTGGACACGGGCATTTGTATCTGAATGG AGTGAATTTCTGTACGAAGAGGACAGGGGCATTCCCCTTAAGGACCGACCGCTGAAATCTGTGTACGGCG ACACTGACTCTCTGTTTGTTACTGAGAAGGGGAGGAGATTGATGGAGAGCCAGGGAAAGAAGCGCATTAA AAAGTACGGAGGAAAGTTGGTCTTCGACCCTAGTTGTCCAGAATTGACATGGAGCGTCGAATGCGAGACC GTGTGTTCTTATTGTGGCGCTGACGCCTACAGCCCGGAGAGCGTATTTCTGGCTCCGAAACTCTATGCGC TGAAGTGTCTGCAGTGTCCTCACTGCGGTAGTACGAGCAAGGGCAAGATTCGAGCCAAAGGTCACGCCAC AGAAGCACTTAGTTATGATCTTATGCTGAAATGCTATCTGGCAGAGGCGCAAGGCGAGGATACTAGGTTC AGCACATCTCGACTCTCACTCAAAAGAACACTCGCTAGTGCCCAACCTGGGGCTCACCCATTCACGGTGA CCGAGACCACCCTCACCAGGACCCTTAGACCATGGAAAGACAAGACTCTGGTTCATCTGGATGCACACAG ACTGGTGCCATATTCTAATAGCCAGCCTAACCCGCGCAATGAGGAGGTGTGTTGGATCGAGATGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC100224 representing AP_000112 Red=Cloning sites Green=Tags MALVQNNRAGSVYTTPTDSRYQSPGSRPCQSTSGSTPSPARASGRHTASPASRRTRTAASSGSSSAPPIL SRRIGKVHRGTVVSPRAHGILHAIDSANGTPLEIKYYLHLQPALTRLCEVNLRAVPADMLFSLTDTMDSS RLYALISRFRPSRAEIWTCRSRGTVTLSVLAFEDPNGSGNAAEEEEDERQLPSGIDFPICFLVRGRQVHL IQQIQPVQRCEHCARFYKHQHECSVRRRDFYFHHINSHSSNWWQEIHFFPIGSHPRTERLFITYDVETYT WMGAFGKQLVPFMLVMKLSGDDNLVKHALQLALELGWDQWEKDSTTFYCLTPEKMKVGQQFRTYRNRLQT SLATDLWMTFLQKNPHLSQWAQEENGLVALEDLSYEDLKRAPAIKGEPRFVELYIVGHNINGFDEIVLAA QVINNRLDVPGPFKISRNFIPRAGKILFNDITFALPNPHYKKRTNFLLWEHGGCDDQDFKYQYLKVMVRD TFALTHTSLRKAAQAYALPVEKGCCPYKAVNQFYMLGSYRADANGFPLEEYWKDKEEYLLNQELWKKKGE KNYNLIGETLNYCALDVLVTASLVEKLRSSYAQFVTDAVGLDAAHFNVFQRPTISSNSHAIFRQILYRAE KPQRTHLGPNILAPSHELYDYVRASIRGGRCYPTYIGVLKEPIYVYDICGMYASALTHPMPWGPPLNPYE RALAVRQWQVALENYTCKIDYFDKNLCPGIFTIDADPPDENQLDVLPPFCSRKGGRLAWTNESLRGEVVT SVDLVTLHNRGWRLRLLSDERTTIFPTWKCLAREYVQLNIAAKERADRDKNQTLRSIAKLLSNALYGSFA TKLDNKKIVFSDQMEESLMKEIAAGRLNIKSSSFIETDTLSTEVMPAFERVYSPNQLALVNSEAEESDED QGPAPFYSPPPENCEHVTYTYKPITFMDAEEGDMCLHTLESSNPLINNDRYPSHVASFVLAWTRAFVSEW SEFLYEEDRGIPLKDRPLKSVYGDTDSLFVTEKGRRLMESQGKKRIKKYGGKLVFDPSCPELTWSVECET VCSYCGADAYSPESVFLAPKLYALKCLQCPHCGSTSKGKIRAKGHATEALSYDLMLKCYLAEAQGEDTRF STSRLSLKRTLASAQPGAHPFTVTETTLTRTLRPWKDKTLVHLDAHRLVPYSNSQPNPRNEEVCWIEMA TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	AC_000005
ORF Size	3567 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AC_000005.1, AP_000112
RefSeq ORF	3567 bp
MW	135.2 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC100219	Myc-DDK-tagged ORF clone of viral ORF for E1A [Human adenovirus A], codon optimized for human cell expression, AP_000107	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100220	Myc-DDK-tagged ORF clone of viral ORF for E1B 19K [Human adenovirus A], codon optimized for human cell expression, AP_000108	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100221	Myc-DDK-tagged ORF clone of viral ORF for E1B 55K [Human adenovirus A], codon optimized for human cell expression, AP_000109	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
VC100222	Myc-DDK-tagged ORF clone of viral ORF for IX [Human adenovirus A], codon optimized for human cell expression, AP_000110	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100223	Myc-DDK-tagged ORF clone of viral ORF for IVa2 [Human adenovirus A], codon optimized for human cell expression, AP_000111	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,510.00
VC100225	Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus A], codon optimized for human cell expression, AP_000113	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
VC100226	Myc-DDK-tagged ORF clone of viral ORF for 52K [Human adenovirus A], codon optimized for human cell expression, AP_000114	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,470.00
VC100227	Myc-DDK-tagged ORF clone of viral ORF for III [Human adenovirus A], codon optimized for human cell expression, AP_000116	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,080.00
VC100228	Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus A], codon optimized for human cell expression, AP_000115	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,030.00
VC100229	Myc-DDK-tagged ORF clone of viral ORF for pVII [Human adenovirus A], codon optimized for human cell expression, AP_000117	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100230	Myc-DDK-tagged ORF clone of viral ORF for V [Human adenovirus A], codon optimized for human cell expression, AP_000118	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,090.00
VC100231	Myc-DDK-tagged ORF clone of viral ORF for pX [Human adenovirus A], codon optimized for human cell expression, AP_000119	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100232	Myc-DDK-tagged ORF clone of viral ORF for pVI [Human adenovirus A], codon optimized for human cell expression, AP_000120	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100233	Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus A], codon optimized for human cell expression, AP_000121	10 ug 产品选项验证失败所有选项都是必需的	CNY 13,870.00
VC100234	Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus A], codon optimized for human cell expression, AP_000122	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100235	Myc-DDK-tagged ORF clone of viral ORF for DBP [Human adenovirus A], codon optimized for human cell expression, AP_000123	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
VC100236	Myc-DDK-tagged ORF clone of viral ORF for 100K [Human adenovirus A], codon optimized for human cell expression, AP_000124	10 ug 产品选项验证失败所有选项都是必需的	CNY 11,880.00
VC100237	Myc-DDK-tagged ORF clone of viral ORF for 22K [Human adenovirus A], codon optimized for human cell expression, AP_000126	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100238	Myc-DDK-tagged ORF clone of viral ORF for 33K [Human adenovirus A], codon optimized for human cell expression, AP_000125	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100239	Myc-DDK-tagged ORF clone of viral ORF for E3 125K [Human adenovirus A], codon optimized for human cell expression, AP_000128	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100240	Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-alpha1 [Human adenovirus A], codon optimized for human cell expression, AP_000129	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100241	Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-beta1 [Human adenovirus A], codon optimized for human cell expression, AP_000130	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100242	Myc-DDK-tagged ORF clone of viral ORF for E3 RID-alpha [Human adenovirus A], codon optimized for human cell expression, AP_000131	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100243	Myc-DDK-tagged ORF clone of viral ORF for E3 RID-beta [Human adenovirus A], codon optimized for human cell expression, AP_000132	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100244	Myc-DDK-tagged ORF clone of viral ORF for E3 147K [Human adenovirus A], codon optimized for human cell expression, AP_000133	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100245	Myc-DDK-tagged ORF clone of viral ORF for pVIII [Human adenovirus A], codon optimized for human cell expression, AP_000127	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100246	Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus A], codon optimized for human cell expression, AP_000135	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,030.00
VC100247	Myc-DDK-tagged ORF clone of viral ORF for U exon, partial [Human adenovirus A], codon optimized for human cell expression, AP_000134	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100248	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF6/7 [Human adenovirus A], codon optimized for human cell expression, AP_000136	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100249	Myc-DDK-tagged ORF clone of viral ORF for E4 34K [Human adenovirus A], codon optimized for human cell expression, AP_000137	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100250	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF4 [Human adenovirus A], codon optimized for human cell expression, AP_000138	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100251	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF3 [Human adenovirus A], codon optimized for human cell expression, AP_000139	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100252	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF2 [Human adenovirus A], codon optimized for human cell expression, AP_000140	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100253	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF1 [Human adenovirus A], codon optimized for human cell expression, AP_000141	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00

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