pTP (AC_000005) Virus Tagged ORF Clone

CAT#: VC100225

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus A], codon optimized for human cell expression, AP_000113



  View other clones from "Virus" (34)

Need custom modification / cloning service?
Get a free quote

CNY 7,700.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100225 represents NCBI reference of AP_000113 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCACTGTCCATTCAGGATTGTGCGCGCCTGACTGGCCAGTCCGTTGCAACCATCGAGAGATTTCTCC
CCTTGCGCAATATCTGGAATCGAGCCCGGGAATTTATGCGCGCCACAACTACGGCCGCCGGAATCACCTG
GATGTCTAGGTATATTTACGAATATCATCGCCTCATGCTTGAAGATCTGGCCCCTGGTGCTGCCGCCACT
CTTGGGTGGCCGCTCTATAGGGAACCACCCCCACACTTCCTGGTAGGCTATCAGTACTTGGTGCGGACCT
GTAACGATTATGTATTCGATTCAAGAGCTTACTCCCGCCTTCGATATACGGAGACCGTACAGCCCGGAAC
GCAGACCGTGAACTGGTCCGTGATGACGAATTGCAGTTACACCATCAATGCCGGAGCTTATCATAGATTT
GTGGACGTCGATGATTTTGCTACGACCCTTACCCAGATTCAACAGGCCGTGTTGGCCGAGCGGGTCGTCG
CCGACCTTGCCCTGCTGCAGCCCCTGCAGGGGTACGGTAGCACGCACATGGCTGACCGGGGCAATCGCGA
AGTCGAGGTCGAGCGACTCATGCAGGACTATTATAAGGACCTGGGACGGTGCCAGAGCGACGTCTGGGGT
ATGGCAGATCGCTTGCGAATTCAGCAAGCTGGACCTAAGGATGTGGTGCTGCTGAAAACAATAAGGGGCC
TGAAGACCGCCTACTTCAATTACATCATCTCATCCATTGCACAGCTTCCCGGAACGCTGGAGGAGACAAA
ATTGTCTTTGCCATGTGATTGTGACTGGCTCGATGCATTCCTGGAGCGGTTCAGCGACCCAGTCGACATG
CAAACACTGTCTAGTCTGAGGGGAGTTCCCACACAACAAATCATTAAGTGTATAATTTCAGCCGTCAGTC
TGCCCAACGCTCCTCGAAACGCTTCATTCCCAATCCTTCACGAGACAGAGTTGCGCGGCGGAGTGTTTGA
GCTTAGACCAAGGGAGAACGGTCGGGCTGTGACCGAAACTATGCGAAGACGACGCGGGGAGATGATTGAG
CGATTTGTGGATCGCCTGCCGGTGCGCCGACGACGCAGACGCCAGCCTCCCCCACCAGTGGTGGAGGAGG
AAATCATGGAAGTGGAGGAGGAGGAGCAAGAGATCGTGCCCGGTGCTTTCCAGCAGGAGGTGCGAGAAAC
AGTGGCTGAACTGATTAGACTGCTTGAGGAGGAGTTGACGGTGGCCGCGAGGAATTCACAATTCTTCAAT
TTTGCGGTAGATTTTTATGAGGCCATGGAGCGCTTGGAGGCATTGGGAGACATCAACGAACTCACACTCA
GACGGTGGATCCTCTATTTCTTCGTCTCCGAGCACGTGGCCACCACTCTGAACTACCTGTTTCAGAGGCT
TCGCAACTACGGCGTGTTTGCCAGACTGGTGGAGCTGAACCTCGCGCAAGTTGTTATGAGAGCTCGAGAT
TCAGAAGGCGGAGTCGTGTATAGCCGAGTGTGGAACGAAATCGGGCTCAACGCTTTCTCCCAGCTGATGA
GTAGGATTTCAAATGACCTCGCTGCTACTATTGAAAGGGCGGGCCATGGAGACTTGCAGGAGGAGGAGAT
CGACCAGCTGATGGCAGAAATCGCTTACCAGGATAACTCCGGCGACGTACAGGAAATTTTGAGACAGGCA
GCCGTGAACGACACTGAAATTGATTCCGTGGAGCTGTCATTCCGCTTCAAAACTACAGGCCCGGTTGTGT
TTACTCAGAGGAGACAGATACAGGACATTAACCGGCGCGTGGTAGCTCACGCCAGCCAGCTTAGAGCACA
GCATCTGCCCCTGCCAGAGAGACAAGCTGACATTCCTCTCCCCCCACTGCCCGCCGGACCAGAGCCACCT
CTCCCCCCGGGAGCCAGACCTCGGAGAAGGTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100225 representing AP_000113
Red=Cloning sites Green=Tags

MALSIQDCARLTGQSVATIERFLPLRNIWNRAREFMRATTTAAGITWMSRYIYEYHRLMLEDLAPGAAAT
LGWPLYREPPPHFLVGYQYLVRTCNDYVFDSRAYSRLRYTETVQPGTQTVNWSVMTNCSYTINAGAYHRF
VDVDDFATTLTQIQQAVLAERVVADLALLQPLQGYGSTHMADRGNREVEVERLMQDYYKDLGRCQSDVWG
MADRLRIQQAGPKDVVLLKTIRGLKTAYFNYIISSIAQLPGTLEETKLSLPCDCDWLDAFLERFSDPVDM
QTLSSLRGVPTQQIIKCIISAVSLPNAPRNASFPILHETELRGGVFELRPRENGRAVTETMRRRRGEMIE
RFVDRLPVRRRRRRQPPPPVVEEEIMEVEEEEQEIVPGAFQQEVRETVAELIRLLEEELTVAARNSQFFN
FAVDFYEAMERLEALGDINELTLRRWILYFFVSEHVATTLNYLFQRLRNYGVFARLVELNLAQVVMRARD
SEGGVVYSRVWNEIGLNAFSQLMSRISNDLAATIERAGHGDLQEEEIDQLMAEIAYQDNSGDVQEILRQA
AVNDTEIDSVELSFRFKTTGPVVFTQRRQIQDINRRVVAHASQLRAQHLPLPERQADIPLPPLPAGPEPP
LPPGARPRRRF

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN AC_000005
ORF Size 1923 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AC_000005.1, AP_000113
RefSeq ORF 1923 bp
MW 73.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...