L3 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100439

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus D], codon optimized for human cell expression, YP_001974453



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CNY 14,160.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100439 represents NCBI reference of YP_001974453 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTACCCCGTCTATGATGCCCCAGTGGGCATATATGCATATTGCCGGCCAGGATGCTTCTGAATACC
TGTCTCCCGGTCTGGTTCAATTCGCACGCGCTACCGACACGTACTTCTCTTTGGGCAATAAATTTAGGAA
TCCAACAGTAGCACCAACACACGACGTGACCACCGATCGGAGCCAGCGCTTGACACTGCGCTTCGTGCCC
GTCGACCGCGAGGACACAACGTACTCCTACAAAGCCCGGTTCACTCTGGCTGTGGGCGACAATAGAGTCC
TGGATATGGCCTCCACTTACTTTGATATTCGAGGAGTGCTGGATCGGGGACCTTCCTTCAAACCTTACTC
CGGGACCGCCTACAATAGCCTGGCTCCTAAAGGCGCCCCAAATAGTTCACAGTGGTTGGCAAAGGATACC
AACGCCGGAGACCAGGCCCTTAAGACCCACACTCACGGAGTGGCAGCCATGGGAGGCACTGACATTACCG
CCAAGGGACTCCAGATCGGGGTTGATACAACAGAAAACAAAAATGAGCCTATTTATGCAAACGAAATTTA
TCAGCCGGAGCCACAGGTTGGTGAAGAAAATTTGCAGGATGTGGAGAACTTTTACGGCGGGCGCGCTCTG
AAGAAGGAGACCAAGATGAAACCTTGCTACGGCAGCTTTGCCAGGCCAACAAACGAAAAGGGAGGACAAG
CTAAATTCCTCACCGACGGCGATGGGCAGCTGACCAAAAACCATGACATTACAATGAATTTCTTCGACAC
CCCAGGGGGGACTGTGGGGCAGGACACAGAGCTGGAAGCTGACATAGTGATGTACGCCGAAAATGTCCAC
CTGGAAACCCCAGATACTCATGTTGTGTACAAACCCGGAACATCCGATGAGTCTTCAGAGGCCAACCTTG
TGCAGCAATCCATGCCAAATAGGCCAAACTATATTGGATTTAGGGACAACTTCGTAGGGCTCATGTACTA
CAACTCCACCGGAAACATGGGGGTCCTTGCAGGTCAGGCCTCACAGCTTAACGCAGTCGTAGACCTGCAG
GACCGCAACACAGAGCTGTCATACCAACTCCTTCTGGATTCTCTGGGCGACAGAACCAGATACTTTTCCA
TGTGGAACAGCGCCGTGGATTCATACGATCCAGACGTGAGGATTATCGAAAACCACGGCGTTGAGGACGA
GTTGCCGAATTACTGCTTCCCCCTGGATGGTGCAGGTACAAACGCTACCTACCAGGGCGTGAAAGTCAAA
AACGGCCAAGACGGTGATGTAAATGCGGATTGGGAGAAGGATCCGAACCTGGCGTCACGCAACCAGATTT
GTAAAGGCAACATATTTGCTATGGAGATTAATCTGCAAGCGAACCTTTGGAAAAGCTTTTTGTACTCTAA
TGTTGCTTTGTATTTGCCCGACAGCTACAAATACACCCCTGCCAACGTGACACTGCCCGCCAATACAAAC
ACGTATGAGTACATGAACGGAAGGGTCGTGGCTCCTTCCCTCGTAGATGCTTATATTAACATCGGCGCAC
GGTGGTCCCTTGATCCCATGGACAACGTTAACCCCTTCAACCACCACCGCAACGCTGGGCTTAGATATAG
ATCCATGTTGCTCGGGAATGGGCGGTACGTGCCGTTTCATATTCAAGTCCCCCAGAAATTCTTTGCGATA
AAAAACCTTCTGCTGCTTCCTGGGAGTTATACTTATGAATGGAACTTTAGGAAAGACGTCAATATGATCC
TGCAATCCAGTCTGGGGAACGATCTGCGAGTTGACGGAGCTTCTGTACGGTTTGACTCAGTGAACCTGTA
CGCCACATTCTTTCCGATGGCACACAACACTGCTTCCACACTGGAAGCCATGCTGCGAAACGACACCAAT
GACCAATCCTTCAACGATTACCTTTCAGCAGCAAATATGCTGTACCCGATCCCTGCCAAGGCCACGAACG
TACCAATATCTATACCTAGCAGAAACTGGGCGGCATTTAGAGGCTGGAGCTTCACCCGGCTGAAGACCAA
GGAAACTCCCTCCTTGGGGAGCGGGTTTGATCCCTACTTTGTGTATTCCGGGTCCATTCCGTACCTGGAT
GGGACATTCTACTTGAACCACACGTTCAAGAAGGTATCAATCATGTTCGACTCTAGTGTCAGCTGGCCAG
GCAACGACCGGCTCCTTACGCCAAACGAATTCGAGATCAAGCGCTCTGTCGACGGGGAAGGATACAACGT
GGCGCAGTGTAATATGACTAAAGACTGGTTTCTCGTGCAGATGCTCTCTCATTACAACATTGGTTACCAG
GGCTTTCATGTGCCGGAGGGCTATAAGGACCGAATGTATTCTTTTTTTCGCAACTTTCAGCCGATGAGTA
GACAGGTCGTTGATGAAATTAATTATAAAGACTATAAAGCTGTCACCTTGCCCTTCCAACACAACAACAG
TGGATTCACCGGGTACCTCGCACCTACCATGAGACAGGGACAGCCCTATCCCGCTAATTTTCCCTACCCG
CTGATTGGTCAGACCGCGGTGCCCTCAGTGACCCAAAAAAAATTTCTGTGCGACCGCGTGATGTGGCGGA
TCCCATTTTCCTCTAATTTCATGTCTATGGGAGCACTCACAGATTTGGGACAAAATATGCTTTACGCCAA
TAGCGCTCACGCCCTGGATATGACATTTGAGGTCGATCCTATGGATGAACCCACACTGCTCTACTTGTTG
TTCGAAGTGTTCGACGTGGTCCGGGTGCATCAGCCACATAGGGGCGTGATCGAAGCTGTCTACTTGAGAA
CTCCCTTTAGCGCCGGCAATGCCACTACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100439 representing YP_001974453
Red=Cloning sites Green=Tags

MATPSMMPQWAYMHIAGQDASEYLSPGLVQFARATDTYFSLGNKFRNPTVAPTHDVTTDRSQRLTLRFVP
VDREDTTYSYKARFTLAVGDNRVLDMASTYFDIRGVLDRGPSFKPYSGTAYNSLAPKGAPNSSQWLAKDT
NAGDQALKTHTHGVAAMGGTDITAKGLQIGVDTTENKNEPIYANEIYQPEPQVGEENLQDVENFYGGRAL
KKETKMKPCYGSFARPTNEKGGQAKFLTDGDGQLTKNHDITMNFFDTPGGTVGQDTELEADIVMYAENVH
LETPDTHVVYKPGTSDESSEANLVQQSMPNRPNYIGFRDNFVGLMYYNSTGNMGVLAGQASQLNAVVDLQ
DRNTELSYQLLLDSLGDRTRYFSMWNSAVDSYDPDVRIIENHGVEDELPNYCFPLDGAGTNATYQGVKVK
NGQDGDVNADWEKDPNLASRNQICKGNIFAMEINLQANLWKSFLYSNVALYLPDSYKYTPANVTLPANTN
TYEYMNGRVVAPSLVDAYINIGARWSLDPMDNVNPFNHHRNAGLRYRSMLLGNGRYVPFHIQVPQKFFAI
KNLLLLPGSYTYEWNFRKDVNMILQSSLGNDLRVDGASVRFDSVNLYATFFPMAHNTASTLEAMLRNDTN
DQSFNDYLSAANMLYPIPAKATNVPISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYFVYSGSIPYLD
GTFYLNHTFKKVSIMFDSSVSWPGNDRLLTPNEFEIKRSVDGEGYNVAQCNMTKDWFLVQMLSHYNIGYQ
GFHVPEGYKDRMYSFFRNFQPMSRQVVDEINYKDYKAVTLPFQHNNSGFTGYLAPTMRQGQPYPANFPYP
LIGQTAVPSVTQKKFLCDRVMWRIPFSSNFMSMGALTDLGQNMLYANSAHALDMTFEVDPMDEPTLLYLL
FEVFDVVRVHQPHRGVIEAVYLRTPFSAGNATT

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_010956
ORF Size 2829 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_010956.1, YP_001974453
RefSeq ORF 2829 bp
Locus ID 6386283
MW 106.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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