IVa2 (NC_010956) Virus Tagged ORF Clone

CAT#: VC100428

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein IVa2 [Human adenovirus D], codon optimized for human cell expression, YP_001974425



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CNY 5,510.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100428 represents NCBI reference of YP_001974425 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAACTCGAGGAAGGCGACCCTGTCCATTCCAGCATCAGCAGGATGAGTCACAGGCACACCCTTGCA
AAAGGCCGGCTCGAGGACCTCCCCTGCACAGAGACGGAGACCATCCCCACTCCGACCCTGAAACTCTGGA
GGGACATGACGCCGGGCGGGCCGGACGACCATCTAGCCGAGCTCTCCAGTCCCAGTCTAGTCAACCGCCA
AAGAGAGGATCATTGTTGGATCGGGATGCCGTGGAGCACGTGACTGAGCTTTGGGATCGCTTGGAGCTTC
TGTCACAGACCCTTGCCAAAATGCCAATGGCAGACGGGCTCAAACCCTTGAAGAACTTTGCCTCCCTGCA
GGAGCTGCTGTCTTTGGGCGGTGATCGCTTGCTCGGAGAACTGGTGCGCGAGAATCTGCAGGTACGAGAC
ATGCTTAATGAAGTTGCCCCCTTGCTGCGAGACGACGGAAGTTGTATGTCACTGAACTATCACCTTCAAC
CCGTAATAGGAGTCATTTACGGCCCAACCGGATGTGGCAAGAGCCAACTGCTCAGGAACCTGCTGTCCTC
ACAGCTGATTACGCCAGCCCCCGAAACAGTGTTTTTTATTGCGCCACAGGTTGATATGATTCCTCCCAGT
GAAATGAAGGCATGGGAGATGCAAATATGTGAGGGAAATTTCGCTCCAGGACCCGAGGGTACCATCGTGC
CACAATCAGGTACCCTTCGCCCCAAGTTCATTAAAATGAGTTACGACGACCTGACACAGGAGCATAACTA
TGATGTGAGCGATCCACGCAACGTATTCGCCAAAGCCGCCGCCCATGGTCCTATCGCCATTATCATGGAT
GAGTGCATGGAAAACCTTGGCGGCCATAAAGGGGTCAGCAAGTTTTTTCACGCCTTTCCAAGCAAATTGC
ATGACAAGTTCCCTAAGTGCACAGGGTACACTGTGCTCGTTGTTCTGCACAACATGAACCCCCGCCGCGA
TCTCGGGGGCAACATTGCGAATCTTAAAATTCAGGCGAAGCTCCATATTATATCCCCAAGAATGCATCCG
TCCCAGCTTAATCGATTCGCCAACACGTACACCAAGGGGCTCCCAGTAGCAATTAGTTTGTTGCTCAAGG
ACATAATCCAGCACCATGCTCAGAGGCCTTGTTACGACTGGATTATATACAATACAACCCCAGAGCACGA
AGCCATGCAGTGGTGTTACCTGCACCCGAGGGACGGTCTCATGCCCATGTACCTGAACATCCAGAGTCAC
CTGTATCGAGTACTTGAAAAAATACATAGGACTCTCAACGACCGGGAGCGCTGGACACGCGCATATCGCG
CTCGAAAGAATAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100428 representing YP_001974425
Red=Cloning sites Green=Tags

METRGRRPCPFQHQQDESQAHPCKRPARGPPLHRDGDHPHSDPETLEGHDAGRAGRPSSRALQSQSSQPP
KRGSLLDRDAVEHVTELWDRLELLSQTLAKMPMADGLKPLKNFASLQELLSLGGDRLLGELVRENLQVRD
MLNEVAPLLRDDGSCMSLNYHLQPVIGVIYGPTGCGKSQLLRNLLSSQLITPAPETVFFIAPQVDMIPPS
EMKAWEMQICEGNFAPGPEGTIVPQSGTLRPKFIKMSYDDLTQEHNYDVSDPRNVFAKAAAHGPIAIIMD
ECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDLGGNIANLKIQAKLHIISPRMHP
SQLNRFANTYTKGLPVAISLLLKDIIQHHAQRPCYDWIIYNTTPEHEAMQWCYLHPRDGLMPMYLNIQSH
LYRVLEKIHRTLNDRERWTRAYRARKNK

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_010956
ORF Size 1344 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_010956.1, YP_001974425
RefSeq ORF 1344 bp
Locus ID 6386270
MW 50.8 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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