L3 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100476

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus E], codon optimized for human cell expression, YP_068032



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CNY 14,060.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100476 represents NCBI reference of YP_068032 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACGCCAAGCATGCTGCCCCAGTGGGCTTATATGCACATCGCCGGGCAGGACGCGAGTGAGTATC
TCAGTCCCGGTCTGGTCCAGTTCGCTCGGGCAACCGATACTTACTTTTCCCTGGGAAACAAGTTTCGGAA
TCCCACGGTCGCTCCCACACACGATGTGACAACGGATAGGAGCCAGAGGCTGACTTTGAGGTTTGTACCT
GTCGATCGGGAAGATAATACTTATAGCTACAAAGTCCGCTACACCCTCGCAGTGGGGGATAACAGGGTTC
TCGATATGGCCTCAACATACTTCGACATCCGGGGAGTACTGGACAGGGGACCTAGTTTTAAGCCCTACAG
TGGCACCGCATACAACAGCCTGGCTCCAAAGGGAGCTCCTAACACGTGTCAGTGGAAAGACTCAGACTCC
AAGATGCATACATTCGGAGCAGCCGCTATGCCTGGAGTAACGGGAAAAAAGATCGAGGCCGATGGACTGC
CAATACGGATCGATTCAACATCTGGGACTGATACAGTTATTTACGCAGACAAGACCTTCCAGCCGGAACC
CCAGGTTGGTAACGACTCCTGGGTGGACACAAACGGGGCCGAAGAAAAATATGGCGGGAGAGCTCTCAAG
GACACAACGAAGATGAAGCCATGTTATGGCTCATTCGCCAAACCCACCAATAAAGAGGGGGGCCAAGCCA
ACCTTAAGGATTCTGAGCCTGCCGCTACGACCCCCAACTACGACATAGACTTGGCCTTTTTTGATTCTAA
AACAATTGTCGCTAATTACGACCCCGATATCGTCATGTACACCGAAAATGTGGACTTGCAGACACCTGAT
ACTCATATCGTGTACAAACCTGGCACAGAGGACACAAGCAGCGAATCCAACCTTGGACAGCAGGCAATGC
CCAACCGACCCAACTACATTGGCTTTAGAGACAACTTCATTGGGTTGATGTACTACAACTCCACTGGAAA
TATGGGCGTGCTCGCAGGACAAGCCTCTCAGCTGAACGCCGTGGTTGACCTGCAAGATCGCAATACTGAG
TTGTCATACCAGCTGCTCCTTGACAGCTTGGGGGATAGAACTCGCTACTTCTCCATGTGGAACCAGGCAG
TCGATTCATATGACCCGGACGTGCGGATAATTGAGAACCACGGGGTGGAGGACGAGCTGCCAAATTACTG
TTTCCCACTGAATGGGGTGGGCCTCACTGATACCTACCAAGGGGTGAAAGTGAAGACAGACGCTGGGAGT
GAGAAGTGGGACAAGGACGACACTACAGTGAGCAACGCTAATGAGATCCACGTGGGAAACCCTTTTGCTA
TGGAAATCAACATTCAGGCCAATCTGTGGAGGAATTTTCTGTATGCAAATGTTGCCCTCTACCTGCCCGA
TAAGTACAAGTACACTCCAGCCAATATCACCCTGCCAACCAATACAAACACCTATGAGTATATGAATGGT
AGAGTCGTGGCGCCATCTCTGGTGGACGCATATATCAATATTGGGGCACGGTGGTCCCTGGACCCCATGG
ACAACGTCAATCCATTTAATCACCATAGGAACGCCGGTCTTAGGTATAGGTCTATGCTGCTGGGTAATGG
ACGCTACGTTCCCTTCCATATACAGGTTCCTCAAAAGTTTTTCGCCATTAAGAGTCTGCTGCTTCTGCCA
GGATCTTACACCTACGAATGGAATTTCCGCAAAGACGTGAACATGATCCTTCAGAGCTCCCTGGGAAATG
ATCTTAGGACTGACGGAGCTAGCATCACCTTTACGTCTATAAATCTCTATGCAACCTTCTTCCCTATGGC
CCATAACACCGCCTCCACTCTCGAAGCTATGTTGCGCAACGACACAAATGATCAGTCATTCAATGATTAT
CTGTCCGCCGCAAACATGCTGTATCCCATCCCCGCAAATGCAACAAACGTTCCCATCAGCATCCCCAGTC
GCAACTGGGCCGCTTTCCGGGGCTGGTCATTCACTAGGCTGAAGACAAAAGAAACACCTTCTCTGGGATC
CGGATTCGACCCCTATTTCGTCTATAGTGGATCAATTCCTTATCTCGATGGCACCTTCTACCTGAACCAT
ACCTTTAAAAAAGTGTCCATCACATTCGACAGTTCCGTATCATGGCCAGGCAACGATAGGTTGCTTACCC
CCAACGAATTTGAAATTAAACGGACTGTAGATGGGGAAGGGTATAACGTGGCACAGTGCAATATGACAAA
AGACTGGTTCCTGGTCCAAATGCTGGCACACTATAACATTGGTTATCAGGGGTTCTATGTTCCGGAAGGA
TATAAAGACAGAATGTATAGCTTCTTCCGAAACTTTCAGCCAATGTCTCGCCAAGTGGTTGACGAAGTCA
ATTACAAAGACTACCAGGCGGTAACCCTCGCTTACCAGCACAATAACAGCGGATTCGTAGGTTACCTTGC
ACCAACTATGAGGCAGGGCCAGCCCTACCCAGCAAACTACCCATACCCACTGATTGGGAAGTCTGCAGTC
ACTAGTGTAACACAAAAAAAGTTTATTTGCGACAGAGTAATGTGGAGAATCCCTTTCAGTAGTAACTTTA
TGTCCATGGGCGCGCTGACAGACCTGGGACAGAACATGTTGTACGCCAACTCAGCCCATGCGTTGGACAT
GAACTTTGAAGTAGACCCGATGGACGAGTCCACACTCCTGTATGTGGTGTTTGAGGTCTTTGATGTCGTT
CGGGTTCACCAACCCCACCGGGGCGTAATCGAGGCTGTCTATCTGAGAACCCCTTTTAGCGCAGGTAATG
CAACCACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100476 representing YP_068032
Red=Cloning sites Green=Tags

MATPSMLPQWAYMHIAGQDASEYLSPGLVQFARATDTYFSLGNKFRNPTVAPTHDVTTDRSQRLTLRFVP
VDREDNTYSYKVRYTLAVGDNRVLDMASTYFDIRGVLDRGPSFKPYSGTAYNSLAPKGAPNTCQWKDSDS
KMHTFGAAAMPGVTGKKIEADGLPIRIDSTSGTDTVIYADKTFQPEPQVGNDSWVDTNGAEEKYGGRALK
DTTKMKPCYGSFAKPTNKEGGQANLKDSEPAATTPNYDIDLAFFDSKTIVANYDPDIVMYTENVDLQTPD
THIVYKPGTEDTSSESNLGQQAMPNRPNYIGFRDNFIGLMYYNSTGNMGVLAGQASQLNAVVDLQDRNTE
LSYQLLLDSLGDRTRYFSMWNQAVDSYDPDVRIIENHGVEDELPNYCFPLNGVGLTDTYQGVKVKTDAGS
EKWDKDDTTVSNANEIHVGNPFAMEINIQANLWRNFLYANVALYLPDKYKYTPANITLPTNTNTYEYMNG
RVVAPSLVDAYINIGARWSLDPMDNVNPFNHHRNAGLRYRSMLLGNGRYVPFHIQVPQKFFAIKSLLLLP
GSYTYEWNFRKDVNMILQSSLGNDLRTDGASITFTSINLYATFFPMAHNTASTLEAMLRNDTNDQSFNDY
LSAANMLYPIPANATNVPISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYFVYSGSIPYLDGTFYLNH
TFKKVSITFDSSVSWPGNDRLLTPNEFEIKRTVDGEGYNVAQCNMTKDWFLVQMLAHYNIGYQGFYVPEG
YKDRMYSFFRNFQPMSRQVVDEVNYKDYQAVTLAYQHNNSGFVGYLAPTMRQGQPYPANYPYPLIGKSAV
TSVTQKKFICDRVMWRIPFSSNFMSMGALTDLGQNMLYANSAHALDMNFEVDPMDESTLLYVVFEVFDVV
RVHQPHRGVIEAVYLRTPFSAGNATT

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_003266
ORF Size 2808 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_003266.2, YP_068032
RefSeq ORF 2808 bp
Locus ID 2958473
MW 105.3 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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