L4 (NC_003266) Virus Tagged ORF Clone

CAT#: VC100479

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for hexon assembly protein 100K [Human adenovirus E], codon optimized for human cell expression, YP_068035



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CNY 12,070.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100479 represents NCBI reference of YP_068035 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAACGCAGCCATCTTCACCTACGTCCCCCTCAGCCCCAACCGCAGATAAGAAACAGCAGCAGAACG
AGAGTCTCACAGCTCCACCCCCCTCCCCAGCCACGTTCGTCGCAGCCCCAGACATGCAGGAGATGGAAGA
GAGTATCCAGATTGATTTGGGATATGTTACCCCCGCGGAGCACGAAGAGGAGCTGGCAGTAAGGTTTTCT
ACCCAAGAGGAGATCCATCAGGAACAACCTGAGCAAGAAGCCAAGTCTGAACACGATTATCTTCACCAAA
GCGGAGGGGAGGATGCACTGATCAAACACCTGGCCCGGCAGGCAATTATCGTCAAAGATGCACTCCTGGA
TAGGACCGAGGTGCCGCTCAGCGTGGAAGAACTGAGTCGCGCCTATGAACTGAATCTGTTTTCACCCAGG
GTGCCTCCCAAGCGACAACCAAATGGCACCTGCGAACCAAACCCACGGCTGAATTTTTACCCCGTCTTCG
CAGTGCCGGAGGCACTCGCCACTTACCACATCTTCTTTAAGAACCAACGAATTCCTGTGAGCTGTCGGGC
GAACAGAACTAGAGCAGATTCATTGTTTAACCTGGGACCTGGAGCTAGACTGCCAGACATCGCTAGTTTG
GAAGAAGTGCCAAAGATCTTTGAAGGATTGGGCTCAGACGAGACGCGCGCAGCAAACGCACTGCAGGGTG
AAGGGGGCGACCATGAGCACCACTCTGCTCTCGTAGAGCTCGAAGGCGATAACGCCCGACTGGCTGTTCT
GAAGCGGACCATTGAACTCACCCATTTTGCTTACCCAGCCCTGAACCTGCCACCAAAGGTTATGTCAACC
GTGATGGACCAGGTGCTCATTAAGCGAGCCTCACCTATTAGCAAGGAAATGCAGGACCCAGAGTCCTCAG
AAGAGGGGAAACCTGTGGTCTCCGACGAGCAACTGGCAAGATGGCTTGGACCACAAGCCAGTCCCCAATC
TCTGGAGGAACGGCGAAAATTGATTATGGCTGTCGTGCTGGTGACTGCCGAACTTGAGTGCCTTAGGCGA
TTCTTCGCCGATGCCGAGATCCTGCGCAAGGTTGAGGAAAATTTGCACTATATATTTAGACACGGTTTTG
TCCGGCAGGCTTGCAAAATCAGTAACGTCGAGCTTACCAACCTCGTTAGCTACATGGGGATACTGCACGA
GAACCGACTGGGGCAAAACGTGCTTCACACTACCCTGAGGGGAGAGGCTAGGCGGGATTACATCAGGGAC
TGCGTTTATCTTTACCTTTGCCATACGTGGCAAACTGCTATGGGCGTGTGGCAACAGTGCCTGGAGGAGC
AGAATCTGAAGGAATTGTGTAAACTTCTCCAAAAGAATCTGAAAGCCCTGTGGACTGGATTTGATGAGCG
AACTACCGCCTCCGACCTGGCAGACCTCATCTTTCCCGAGAGACTGCGACTGACCCTGCGAAACGGGCTT
CCTGACTTCATGTCCCAGAGTATGCTGCAGAATTTCCGGTCTTTTATCCTGGAGCGCTCAGGAATACTGC
CAGCCACCTGCTCCGCACTCCCTAGTGATTTTGTGCCACTGACTTTTCGGGAATGTCCCCCTCCTTTGTG
GTCTCACTGCTACCTCCTCAGACTCGCAAACTATCTTGCGTATCACTCAGACGTGATTGAGGACGTCAGT
AGCGAGGGCCTGCTGGAGTGTCATTGCCGCTGTAATCTCTGTACACCCCACAGGTCCCTGGCATGCAATC
CTCAGCTCTTGTCTGAGACACAGATCATCGGAACATTTGAACTCCAGGGACCAGGCGACGAGGGCAGCGC
GGCTAAGGGCGGACTGAAGCTTACACCTGGGTTGTGGACCTCCGCCTACCTGCGCAAATTCGTACCAGAA
GACTACCACCCCTTTGAAATCAGATTTTATGAAGACCAATCCCAGCCACCAAAGGCTGAGCTTAGCGCCT
GTGTGATCACACAGGGAGCGATTCTTGCCCAACTCCAGGCCATCCAGAAGTCACGGCAGGAGTTCCTCTT
GAAAAAAGGCCGAGGAGTGTACCTCGACCCTCAGACGGGCGAGGAACTGAATCCGGGCTTTCCACAGGAT
GCGCCCCGGAAGCAACAGGAAGCTGAAAGTGGTGCAGCCGCTAGAGGGGGTTTTGGTGGAAGGCTTGGAG
AGCAATCCGGAAGAGGGGGAGGTGATGGAAGACTGGGTCAACACTCAGGTCGCGGCCAACCTGCCCGGCA
GTCTGGAAGAAGGGGGGGGGGTAGAGGGGGTGGGAGGTCCAGCCGCCGCAGGCAGACAGTGGTACTGGGT
GGTGAGTCTAAACAGCACGGTTACCACCTGCGGTCAGGGTCTGGCAGTCGGTCCACTCCTCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100479 representing YP_068035
Red=Cloning sites Green=Tags

METQPSSPTSPSAPTADKKQQQNESLTAPPPSPATFVAAPDMQEMEESIQIDLGYVTPAEHEEELAVRFS
TQEEIHQEQPEQEAKSEHDYLHQSGGEDALIKHLARQAIIVKDALLDRTEVPLSVEELSRAYELNLFSPR
VPPKRQPNGTCEPNPRLNFYPVFAVPEALATYHIFFKNQRIPVSCRANRTRADSLFNLGPGARLPDIASL
EEVPKIFEGLGSDETRAANALQGEGGDHEHHSALVELEGDNARLAVLKRTIELTHFAYPALNLPPKVMST
VMDQVLIKRASPISKEMQDPESSEEGKPVVSDEQLARWLGPQASPQSLEERRKLIMAVVLVTAELECLRR
FFADAEILRKVEENLHYIFRHGFVRQACKISNVELTNLVSYMGILHENRLGQNVLHTTLRGEARRDYIRD
CVYLYLCHTWQTAMGVWQQCLEEQNLKELCKLLQKNLKALWTGFDERTTASDLADLIFPERLRLTLRNGL
PDFMSQSMLQNFRSFILERSGILPATCSALPSDFVPLTFRECPPPLWSHCYLLRLANYLAYHSDVIEDVS
SEGLLECHCRCNLCTPHRSLACNPQLLSETQIIGTFELQGPGDEGSAAKGGLKLTPGLWTSAYLRKFVPE
DYHPFEIRFYEDQSQPPKAELSACVITQGAILAQLQAIQKSRQEFLLKKGRGVYLDPQTGEELNPGFPQD
APRKQQEAESGAAARGGFGGRLGEQSGRGGGDGRLGQHSGRGQPARQSGRRGGGRGGGRSSRRRQTVVLG
GESKQHGYHLRSGSGSRSTPQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_003266
ORF Size 2373 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_003266.2, YP_068035
RefSeq ORF 2373 bp
Locus ID 2958479
MW 88.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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