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E1A (NC_001454) Virus Tagged ORF Clone
CAT＃: VC100500
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for control protein E1A [Human adenovirus F], codon optimized for human cell expression, NP_040845
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CNY 3,800.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC100500 represents NCBI reference of NP_040845 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGATGCTTCCTGATTTTTTTACAGGCAATTGGGATGACATGTTCCAGGGACTGCTCGAGACAGAGT ACGTGTTCGATTTTCCGGAACCCTCTGAGGCATCTGAAGAAATGTCTCTGCACGACTTGTTCGATGTGGA AGTCGATGGCTTCGAGGAAGATGCCAATCAGGAAGCAGTCGATGGTATGTTCCCGGAGAGGCTCCTGTCT GAAGCAGAATCTGCAGCAGAGTCTGGCAGTGGAGACAGCGGTGTCGGCGAGGAACTTCTGCCTGTCGATC TGGACCTGAAGTGCTATGAGGATGGCTTGCCCCCAAGCGACCCCGAAACTGACGAGGCAACAGAGGCAGA GGAAGAAGCCGCAATGCCCACATACGTGAATGAAAATGAGAACGAACTGGTGCTTGACTGCCCTGAGAAC CCCGGTCGCGGATGCAGGGCATGTGACTTCCACCGGGGGACTAGTGGGAACCCCGAGGCAATGTGTGCTT TGTGCTATATGCGGCTGACCGGGCACTGCATATATTCCCCAATTTCCGATGCCGAGGGTGAGAGCGAATC AGGCAGCCCAGAGGACACGGATTTTCCGCACCCCCTTACCGCAACCCCTCCTCACGGCATCGTCCGCACA ATTCCTTGCCGCGTCTCTTGTCGCAGGCGGCCCGCCGTGGAGTGCATTGAAGATCTCTTGGAAGAGGACC CTACCGACGAACCACTCAATCTTAGCCTGAAGAGGCCTAAGTGTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100500 representing NP_040845
Red=Cloning sites Green=Tags MRMLPDFFTGNWDDMFQGLLETEYVFDFPEPSEASEEMSLHDLFDVEVDGFEEDANQEAVDGMFPERLLS EAESAAESGSGDSGVGEELLPVDLDLKCYEDGLPPSDPETDEATEAEEEAAMPTYVNENENELVLDCPEN PGRGCRACDFHRGTSGNPEAMCALCYMRLTGHCIYSPISDAEGESESGSPEDTDFPHPLTATPPHGIVRT IPCRVSCRRRPAVECIEDLLEEDPTDEPLNLSLKRPKCS TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001454 |
| ORF Size | 747 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001454.1, NP_040845 |
| RefSeq ORF | 747 bp |
| Locus ID | 2715915 |
| MW | 27.5 kDa |
Documents
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Resources
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| VC100514 | Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus F], codon optimized for human cell expression, NP_040862 |
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| VC100515 | Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus F], codon optimized for human cell expression, NP_040863 |
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| VC100516 | Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human adenovirus F], codon optimized for human cell expression, NP_040864 |
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| VC100517 | Myc-DDK-tagged ORF clone of viral ORF for hexon assembly protein 100K [Human adenovirus F], codon optimized for human cell expression, NP_040865 |
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| VC100518 | Myc-DDK-tagged ORF clone of viral ORF for protein 33K [Human adenovirus F], codon optimized for human cell expression, NP_040866 |
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| VC100519 | Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein 22K [Human adenovirus F], codon optimized for human cell expression, NP_040867 |
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| VC100520 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pVIII [Human adenovirus F], codon optimized for human cell expression, NP_040868 |
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| VC100521 | Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-alpha [Human adenovirus F], codon optimized for human cell expression, NP_040869 |
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| VC100522 | Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-beta [Human adenovirus F], codon optimized for human cell expression, NP_040870 |
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| VC100523 | Myc-DDK-tagged ORF clone of viral ORF for membrane protein E3 RID-alpha [Human adenovirus F], codon optimized for human cell expression, NP_040871 |
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| VC100524 | Myc-DDK-tagged ORF clone of viral ORF for membrane protein E3 RID-beta [Human adenovirus F], codon optimized for human cell expression, NP_040872 |
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| VC100525 | Myc-DDK-tagged ORF clone of viral ORF for control protein E3 147K [Human adenovirus F], codon optimized for human cell expression, NP_040873 |
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| VC100526 | Myc-DDK-tagged ORF clone of viral ORF for protein U, partial [Human adenovirus F], codon optimized for human cell expression, NP_040874 |
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| VC100527 | Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus F], codon optimized for human cell expression, NP_040875 |
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| VC100528 | Myc-DDK-tagged ORF clone of viral ORF for fiber-2 [Human adenovirus F], codon optimized for human cell expression, NP_040876 |
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| VC100529 | Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf6/7 [Human adenovirus F], codon optimized for human cell expression, NP_040877 |
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| VC100530 | Myc-DDK-tagged ORF clone of viral ORF for control protein E4 34K [Human adenovirus F], codon optimized for human cell expression, NP_040878 |
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| VC100531 | Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf4 [Human adenovirus F], codon optimized for human cell expression, NP_040879 |
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| VC100532 | Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf3 [Human adenovirus F], codon optimized for human cell expression, NP_040880 |
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| VC100533 | Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf2 [Human adenovirus F], codon optimized for human cell expression, NP_040881 |
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