L3 (NC_001454) Virus Tagged ORF Clone

CAT#: VC100514

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus F], codon optimized for human cell expression, NP_040862



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CNY 13,870.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100514 represents NCBI reference of NP_040862 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTACCCCATCCATGATGCCACAGTGGAGCTACATGCACATAGCTGGACAGGATGCCTCCGAATACC
TTTCTCCCGGACTGGTCCAATTTGCTCGCGCCACCGACACATATTTCAGTCTCGGCAACAAATTTCGCAA
TCCTACTGTTGCACCGACCCATGATGTGACCACTGATCGCTCCCAGCGCCTTACCCTGCGATTTGTGCCT
GTGGATAGGGAAGAGACCGCATACTCCTACAAGGTAAGATTTACACTGGCCGTAGGAGACAATCGCGTTC
TGGATATGGCTAGCACTTACTTCGATATCAGGGGAGTACTGGACAGAGGTCCTTCTTTTAAGCCTTATTC
TGGAACTGCCTACAACTCCCTGGCTCCCAAGGGGGCACCAAACCCCTCACAGTGGACCAACCAGAATAAG
ACTAATTCATTCGGCCAGGCTCCATATATTGGGCAAAAAATTACAAACCAGGGGGTTCAGGTCGGATCCG
ATTCCAACAACAGAGACGTGTTTGCAGATAAGACTTATCAACCAGAGCCTCAGGTCGGGCAGACGCAGTG
GAACATTAACCCCATGCAAAACGCCGCCGGCAGAATCCTGAAACAAACGACGCCAATGCAGCCTTGTTAC
GGAAGCTACGCTAGACCAACAAATGAAAAGGGGGGGCAAGCTAAGTTGGTTAAGAATGACGACAACCAAA
CCACCACTACGAACGTAGGGCTCAACTTCTTCACTACAGCCACCGAGACGGCCAACTTTAGCCCCAAAGT
GGTCCTCTATTCTGAGGATGTCAACCTCGAGGCCCCTGATACTCACCTGGTTTTCAAGCCCGACGTGAAT
GGGACTAGCGCCGAACTGCTGCTGGGTCAGCAGGCCGCGCCCAATAGGCCAAACTACATTGGGTTTAGAG
ATAACTTTATTGGTCTTATGTATTACAACTCCACCGGGAATATGGGCGTACTCGCTGGACAGGCCTCCCA
GCTCAATGCCGTTGTGGATCTGCAGGACAGAAATACCGAACTGTCCTACCAGCTGATGCTGGATGCTCTG
GGAGATCGGTCAAGGTATTTTAGCATGTGGAACCAAGCCGTAGACAGTTACGACCCCGACGTGCGCATTA
TTGAGAATCATGGCGTAGAGGATGAGCTGCCTAATTACTGTTTTCCACTGAATGGACAGGGCATAAGCAA
TTCATACCAGGGCGTTAAGACAGACAATGGGACGAACTGGTCACAAAATAATACAGACGTGTCCAGCAAT
AATGAGATCAGCATTGGCAACGTCTTTGCAATGGAGATCAACTTGGCGGCCAACCTGTGGCGGTCCTTTC
TGTATTCTAATGTAGCTCTGTACCTGCCCGACAGTTACAAGATCACACCAGATAACATCACTTTGCCCGA
TAACAAGAATACTTACGCCTACATGAACGGCAGAGTGGCCGTCCCTTCAGCACTCGACACATACGTGAAC
ATTGGAGCTAGATGGTCCCCGGATCCTATGGACAATGTGAACCCGTTTAATCATCACCGAAACGCAGGCC
TGCGATACCGCAGCATGTTGCTGGGCAACGGCAGATACGTGCCTTTTCATATTCAGGTGCCCCAAAAATT
CTTCGCCATAAAAAACCTCCTTCTGCTCCCCGGGTCATATACGTACGAGTGGAATTTCCGAAAAGATGTA
AACATGATTCTGCAGAGCTCCCTGGGGAACGATCTGCGGGTGGACGGGGCATCCGTTAGATTTGACAGCA
TTAATCTGTATGCAAATTTCTTTCCGATGGCCCATAACACCGCCAGCACACTCGAAGCAATGCTCAGAAA
TGATACTAACGACCAGTCATTTAATGATTATCTTTGCGCAGCCAATATGTTGTACCCCATCCCAGCTAAT
GCCACCAGCGTCCCTATTAGCATTCCATCCAGGAATTGGGCCGCTTTCCGGGGCTGGTCCTTCACAAGAC
TGAAGACTAAAGAGACTCCCTCCCTCGGTAGCGGGTTCGACCCCTACTTTACCTATTCTGGTTCAGTGCC
TTATCTGGACGGGACCTTCTACCTCAACCATACATTTAAAAAGGTCAGCGTCATGTTCGACTCTAGTGTC
TCCTGGCCAGGCAACGATAGACTGTTGACACCAAACGAGTTCGAAATTAAGAGGACAGTCGATGGGGAGG
GCTACAACGTGGCTCAATGTAACATGACAAAGGATTGGTTCCTTATTCAAATGTTGAGCCACTATAATAT
TGGCTACCAGGGGTTCCACGTCCCAGAGTCTTACAAGGACCGCATGTATTCCTTTTTTCGAAACTTCCAA
CCCATGTCCCGCCAAGTGGTAGATACTACAACCTACACAGAATACCAGAATGTAACGCTGCCCTTCCAGC
ATAATAATTCAGGCTTTGTGGGTTACATGGGTCCAGCAATAAGAGAAGGCCAGGCCTACCCAGCAAACTA
CCCATATCCCCTTATTGGACAAACCGCCGTTCCGAGTCTCACACAAAAAAAATTCCTGTGCGATAGGACA
ATGTGGAGAATTCCTTTTTCAAGTAATTTCATGTCTATGGGCGCTCTGACCGACCTCGGGCAAAATATGC
TTTACGCCAATAGCGCTCATGCGCTGGACATGACCTTTGAGGTGGACCCTATGGATGAACCCACCCTTCT
GTATGTTCTCTTCGAGGTGTTTGACGTTGTCCGGATACACCAGCCCCATAGAGGGGTAATCGAGGCCGTA
TACTTGCGGACTCCTTTCTCCGCCGGTAACGCCACGACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100514 representing NP_040862
Red=Cloning sites Green=Tags

MATPSMMPQWSYMHIAGQDASEYLSPGLVQFARATDTYFSLGNKFRNPTVAPTHDVTTDRSQRLTLRFVP
VDREETAYSYKVRFTLAVGDNRVLDMASTYFDIRGVLDRGPSFKPYSGTAYNSLAPKGAPNPSQWTNQNK
TNSFGQAPYIGQKITNQGVQVGSDSNNRDVFADKTYQPEPQVGQTQWNINPMQNAAGRILKQTTPMQPCY
GSYARPTNEKGGQAKLVKNDDNQTTTTNVGLNFFTTATETANFSPKVVLYSEDVNLEAPDTHLVFKPDVN
GTSAELLLGQQAAPNRPNYIGFRDNFIGLMYYNSTGNMGVLAGQASQLNAVVDLQDRNTELSYQLMLDAL
GDRSRYFSMWNQAVDSYDPDVRIIENHGVEDELPNYCFPLNGQGISNSYQGVKTDNGTNWSQNNTDVSSN
NEISIGNVFAMEINLAANLWRSFLYSNVALYLPDSYKITPDNITLPDNKNTYAYMNGRVAVPSALDTYVN
IGARWSPDPMDNVNPFNHHRNAGLRYRSMLLGNGRYVPFHIQVPQKFFAIKNLLLLPGSYTYEWNFRKDV
NMILQSSLGNDLRVDGASVRFDSINLYANFFPMAHNTASTLEAMLRNDTNDQSFNDYLCAANMLYPIPAN
ATSVPISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYFTYSGSVPYLDGTFYLNHTFKKVSVMFDSSV
SWPGNDRLLTPNEFEIKRTVDGEGYNVAQCNMTKDWFLIQMLSHYNIGYQGFHVPESYKDRMYSFFRNFQ
PMSRQVVDTTTYTEYQNVTLPFQHNNSGFVGYMGPAIREGQAYPANYPYPLIGQTAVPSLTQKKFLCDRT
MWRIPFSSNFMSMGALTDLGQNMLYANSAHALDMTFEVDPMDEPTLLYVLFEVFDVVRIHQPHRGVIEAV
YLRTPFSAGNATT

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001454
ORF Size 2769 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001454.1, NP_040862
RefSeq ORF 2769 bp
Locus ID 2715932
MW 104.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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