NS1 (NC_007455) Virus Tagged ORF Clone
CAT#: VC100557
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for NS1 [Human bocavirus], codon optimized for human cell expression, YP_338086
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CNY 7,700.00
Product images
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CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC100557 represents NCBI reference of YP_338086 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATTCAATCCGCCTGTGATAAGAGCCTTCAGCCAGCCTGCCTTTACTTATGTGTTTAAGTTCCCCT ATCCACAGTGGAAAGAGAAAGAGTGGCTGCTTCACGCCCTCCTGGCCCACGGAACCGAGCAGTCCATGAT TCAGCTGCGAAACTGCGCCCCTCATCCAGACGAAGACATTATTCGAGATGACCTTTTGATCTCACTGGAA GACCGGCATTTTGGTGCAGTACTGTGTAAAGCCGTTTATATGGCCACAACTACACTTATGTCCCACAAGC AGCGAAACATGTTCCCCAGATGTGATATCATTGTGCAGTCCGAACTCGGCGAGAAAAATCTGCACTGTCA CATTATAGTGGGAGGCGAGGGCCTGAGCAAGCGCAACGCAAAGTCAAGTTGTGCACAATTCTATGGACTG ATTTTGGCTGAGATAATTCAGCGCTGTAAATCCCTTTTGGCAACCCGCCCCTTCGAACCCGAGGAAGCTG ACATTTTTCATACTCTGAAAAAGGCTGAAAGAGAGGCATGGGGCGGAGTGACAGGCGGAAACATGCAGAT CTTGCAGTATAGGGACAGACGAGGCGACCTCCACGCTCAAACAGTAGATCCCCTGCGGTTCTTCAAGAAT TATCTGCTTCCCAAAAATAGGTGTATCTCCTCATACTCCAAGCCCGATGTGTGCACGTCACCTGACAACT GGTTCATTCTGGCCGAAAAGACTTACAGCCATACTTTGATAAACGGACTGCCTCTGCCGGAGCACTATCG GAAGAATTATCACGCCACTCTGGACAACGAGGTAATACCGGGACCTCAGACGATGGCCTACGGAGGCAGG GGACCCTGGGAGCACCTGCCCGAAGTGGGCGATCAGCGGCTCGCAGCAAGCAGCGTGTCCACAACTTATA AACCAAATAAGAAAGAAAAGCTTATGCTGAACCTTCTGGACAAGTGCAAGGAACTGAACTTGTTGGTTTA TGAAGACCTCGTGGCAAATTGCCCTGAGCTTCTCCTGATGCTGGAAGGCCAGCCTGGCGGGGCTAGACTT ATTGAACAGGTGTTGGGCATGCACCACATTAATGTGTGCTCTAACTTCACCGCGCTTACCTACCTCTTCC ACCTGCACCCTGTGACTTCCCTGGACTCCGACAATAAGGCACTGCAGCTGCTGCTGATCCAGGGGTACAA TCCTCTTGCAGTAGGCCACGCTCTGTGTTGCGTACTCAACAAACAGTTTGGGAAGCAAAACACTGTGTGC TTTTATGGTCCGGCATCCACTGGGAAGACCAACATGGCAAAAGCTATTGTCCAGGGGATCCGGCTCTATG GTTGTGTGAACCATCTGAACAAAGGGTTTGTGTTTAATGACTGTCGCCAGAGGCTGGTCGTGTGGTGGGA AGAGTGTCTGATGCATCAAGACTGGGTGGAACCAGCCAAGTGCATCCTTGGCGGAACCGAATGCAGGATT GACGTGAAGCACAGGGACAGCGTCCTGCTGACACAAACCCCGGTAATCATCAGCACGAATCACGACATTT ATGCCGTGGTCGGAGGCAACTCCGTGTCACACGTACATGCGGCGCCCCTTAAAGAACGGGTGATTCAACT TAACTTTATGAAGCAGCTCCCGCAGACATTCGGGGAGATCACTGCCACTGAGATTGCCGCGCTTCTTCAG TGGTGCTTTAACGAGTACGATTGTACTCTCACAGGCTTCAAGCAAAAGTGGAATCTTGACAAGATTCCAA ATTCATTTCCACTGGGAGTGCTCTGTCCGACCCATTCCCAGGATTTTACCTTGCATGAGAACGGCTATTG TACTGACTGCGGGGGCTACCTCCCCCACAGCGCTGATAATAGCATGTACACAGATCGCGCGTCCGAAACA AGCACAGGAGACATCACTCCCTCAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100557 representing YP_338086
Red=Cloning sites Green=Tags MAFNPPVIRAFSQPAFTYVFKFPYPQWKEKEWLLHALLAHGTEQSMIQLRNCAPHPDEDIIRDDLLISLE DRHFGAVLCKAVYMATTTLMSHKQRNMFPRCDIIVQSELGEKNLHCHIIVGGEGLSKRNAKSSCAQFYGL ILAEIIQRCKSLLATRPFEPEEADIFHTLKKAEREAWGGVTGGNMQILQYRDRRGDLHAQTVDPLRFFKN YLLPKNRCISSYSKPDVCTSPDNWFILAEKTYSHTLINGLPLPEHYRKNYHATLDNEVIPGPQTMAYGGR GPWEHLPEVGDQRLAASSVSTTYKPNKKEKLMLNLLDKCKELNLLVYEDLVANCPELLLMLEGQPGGARL IEQVLGMHHINVCSNFTALTYLFHLHPVTSLDSDNKALQLLLIQGYNPLAVGHALCCVLNKQFGKQNTVC FYGPASTGKTNMAKAIVQGIRLYGCVNHLNKGFVFNDCRQRLVVWWEECLMHQDWVEPAKCILGGTECRI DVKHRDSVLLTQTPVIISTNHDIYAVVGGNSVSHVHAAPLKERVIQLNFMKQLPQTFGEITATEIAALLQ WCFNEYDCTLTGFKQKWNLDKIPNSFPLGVLCPTHSQDFTLHENGYCTDCGGYLPHSADNSMYTDRASET STGDITPSK TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_007455 |
ORF Size | 1917 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NC_007455.1, YP_338086 |
RefSeq ORF | 1917 bp |
Locus ID | 3711585 |
MW | 72.1 kDa |
Documents
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FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
VC100558 | Myc-DDK-tagged ORF clone of viral ORF for NP-1 [Human bocavirus], codon optimized for human cell expression, YP_338087 |
CNY 3,800.00 |
|
VC100559 | Myc-DDK-tagged ORF clone of viral ORF for VP1 [Human bocavirus], codon optimized for human cell expression, YP_338088 |
CNY 10,170.00 |
|
VC100560 | Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human bocavirus], codon optimized for human cell expression, YP_338089 |
CNY 6,560.00 |