HCoV-NL63 N Gene Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC100597 represents NCBI reference of YP_003771 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCGTGAACTGGGCTGATGACAGAGCTGCGAGGAAGAAGTTCCCCCCCCCATCCTTTTACATGC CCCTGCTGGTTTCCTCTGACAAGGCTCCCTACAGGGTGATCCCGCGCAACCTGGTTCCTATCGGCAAGGG AAACAAGGACGAGCAGATAGGATATTGGAATGTCCAGGAGCGCTGGCGCATGCGCAGAGGGCAGCGGGTG GATCTCCCGCCTAAAGTCCACTTCTACTACCTCGGAACAGGACCACACAAGGATCTGAAATTCCGACAAC GGAGCGATGGAGTTGTGTGGGTGGCAAAGGAGGGGGCCAAGACAGTGAATACCTCTCTTGGTAACCGGAA ACGGAATCAGAAGCCCCTGGAGCCCAAATTCAGCATAGCACTCCCCCCAGAGCTCTCAGTAGTCGAATTT GAAGATAGAAGCAACAACAGCAGCCGCGCAAGCAGTCGATCCAGCACAAGGAATAACTCTAGGGACTCTT CAAGAAGTACGTCCCGCCAACAGTCCCGAACGAGGTCAGATAGTAATCAGTCCAGCTCCGACCTCGTGGC AGCTGTGACCTTGGCCCTCAAGAACCTCGGATTCGACAATCAGTCCAAGTCTCCTTCCTCTAGCGGGACA TCAACGCCTAAGAAACCTAACAAACCCCTGTCCCAGCCTAGAGCAGACAAGCCTAGCCAGCTTAAAAAGC CTCGGTGGAAGCGGGTCCCCACAAGAGAAGAGAACGTGATTCAGTGCTTCGGTCCACGGGACTTCAATCA TAACATGGGCGACTCTGATCTCGTACAGAACGGCGTCGACGCTAAGGGGTTTCCCCAGCTTGCCGAGCTG ATTCCAAATCAGGCAGCACTTTTCTTCGATAGCGAAGTTTCAACTGACGAGGTGGGCGACAACGTCCAGA TAACTTACACCTACAAGATGCTTGTAGCTAAGGACAACAAAAATCTGCCCAAGTTCATCGAGCAAATAAG CGCCTTTACTAAACCCTCAAGCATCAAAGAAATGCAGTCCCAGAGTTCACACGTTGCCCAGAACACCGTC CTGAATGCGTCAATTCCGGAGAGCAAACCTCTTGCTGACGATGACTCTGCCATCATCGAAATTGTCAACG AGGTCTTGCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC100597 representing YP_003771 Red=Cloning sites Green=Tags MASVNWADDRAARKKFPPPSFYMPLLVSSDKAPYRVIPRNLVPIGKGNKDEQIGYWNVQERWRMRRGQRV DLPPKVHFYYLGTGPHKDLKFRQRSDGVVWVAKEGAKTVNTSLGNRKRNQKPLEPKFSIALPPELSVVEF EDRSNNSSRASSRSSTRNNSRDSSRSTSRQQSRTRSDSNQSSSDLVAAVTLALKNLGFDNQSKSPSSSGT STPKKPNKPLSQPRADKPSQLKKPRWKRVPTREENVIQCFGPRDFNHNMGDSDLVQNGVDAKGFPQLAEL IPNQAALFFDSEVSTDEVGDNVQITYTYKMLVAKDNKNLPKFIEQISAFTKPSSIKEMQSQSSHVAQNTV LNASIPESKPLADDDSAIIEIVNEVLH TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_005831
ORF Size	1131 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_005831.2, YP_003771
RefSeq ORF	1131 bp
Locus ID	2943504
MW	42.3 kDa

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