NS1 (NC_004295) Virus Tagged ORF Clone
CAT#: VC100780
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for NS1 protein [Human erythrovirus V9], codon optimized for human cell expression, NP_694863
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CNY 10,170.00
Product images
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CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC100780 represents NCBI reference of NP_694863 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTGTTTAGGGGTGTTCTGCATATCTCTTCCAATATTCTCGACTGTGCAAATGATAACTGGTGGT GCTCCATGTTGGATCTGGATACAAGTGATTGGGAACCACTGACTCACAGTAACCGACTGATGGCCATTTA TCTGTCATCTGTGGCGAGCAAGCTGGACTTCACAGGAGGCCCCCTTGCAGGCTGTCTGTATTTTTTTCAA GTGGAATGTAATAAATTCGAGGAGGGATACCACATCCATGTAGTGATTGGTGGACCCGGCCTGAACGCCA GGAACTTGACCGTCTGTGTGGAAGGCCTTTTTAACAACGTGCTCTATCATCTGGTGACTGAGTCCGTCAA GCTGAAGTTCCTGCCGGGAATGACCACAAAAGGCAAGTACTTCAGGGACGGCGAGCAATTCATTGAAAAC TATCTCATGAAAAAAATACCCCTGAATGTGGTCTGGTGTGTCACAAATATAGATGGGTACATAGACACCT GTATTAGCGCCTCCTTTAGACGCGGGGCTTGCCATGCAAAAAGGCCACGGATCACTGCTAATACAGACTC AGCAACCAATGAGACAGGTGAGTCATCTTGTGGCGGCGGTGACGTCGTTCCGTTTGCCGGAAAGGGCACC AAGGCTGGCCTGAAATTTCAGACAATGGTTAACTGGCTGTGCGAGAACCGCGTCTTCACGGAGGATAAAT GGAAATTGGTCGATTTCAATCAGTATACTCTGTTGTCAAGCAGCCATTCCGGCAGCTTCCAGATCCAGTC TGCTCTGAAGCTGGCGATATATAAGGCTACAAACCTGGTTCCTACTTCAACCTTCCTTCTGCATAGTGAT TTCGAGCAAGTTACCTGTATAAAGGAGAATAAGATCGTGAAGCTGCTGCTGTGTCAGAACTACGATCCTC TGCTCGTGGGACAACACGTCTTGAGGTGGATCGACAAAAAGTGCGGGAAGAAAAACACACTGTGGTTCTA TGGCCCCCCCTCCACCGGGAAGACCAATCTTGCCATGGCCATTGCTAAAACAGTGCCGGTTTACGGCATG GTTAATTGGAACAATGAGAATTTCCCCTTCAATGACGTGGCGGGAAAGAGCTTGGTCGTGTGGGACGAGG GAATCATCAAGTCAACGATCGTCGAGGCAGCCAAGGCAATTTTGGGTGGCCAGCCAACCCGGGTAGACCA GAAAATGAGAGGTTCCGTAGCCGTGCCCGGCGTCCCCGTGGTGATCACTTCTAACGGTGACATTACCTTT GTAGTGTCTGGCAACACCACCACCACAGTACACGCTAAGGCCCTGAAGGAGCGCATGGTGAAGCTGAACT TCACTATTAGGTGCAGCCCTGACATGGGCTTGCTTACGGAAGCTGATGTGCAGCAATGGCTTACGTGGTG CAACGCTCAATCATGGTCTCATTACGAGAACTGGGCGATCAATTACACGTTCGACTTCCCAGGAATTAAC GCAGACGCCTTGCATCCTGATTTGCAGACCACACCCATCGTCCCGGACACCAGCATATCTAGTTCTGGCG GCGAGAGCTCCGAAGAACTGTCCGAATCCTCCTTCTTCAATCTTATCACGCCTGGGGCATGGAACTCCGA AACACCCCGGTCCTCCACCCCAGTGCCCGGAACTAGTAGCGGAGAGAGCTTTGTAGGCTCCCCAGTGAGT TCAGAAGTAGTGGCCGCAAGCTGGGAGGAGGCTTTTTACACACCTCTGGCCGATCAGTTTCGCGAGCTGC TTGTGGGTGTCGATTTCGTCTGGGATGGTGTGAGGGGGTTGCCCGTCTGTTGTGTGGAACATATTAACAA TAGTGGGGGAGGGCTGGGCTTGTGTCCTCACTGTATCAACGTGGGCGCATGGTATAATGGCTGGAAATTT CGGGAATTCACTCCGGACTTGGTCCGGTGTTCCTGCCATGTTGGCGCTAGTAATCCATTTTCCGTGCTTA CTTGTAAGAAATGTGCCTATCTTAGTGGCCTCCAATCCTTTGTTGACTACGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100780 representing NP_694863
Red=Cloning sites Green=Tags MELFRGVLHISSNILDCANDNWWCSMLDLDTSDWEPLTHSNRLMAIYLSSVASKLDFTGGPLAGCLYFFQ VECNKFEEGYHIHVVIGGPGLNARNLTVCVEGLFNNVLYHLVTESVKLKFLPGMTTKGKYFRDGEQFIEN YLMKKIPLNVVWCVTNIDGYIDTCISASFRRGACHAKRPRITANTDSATNETGESSCGGGDVVPFAGKGT KAGLKFQTMVNWLCENRVFTEDKWKLVDFNQYTLLSSSHSGSFQIQSALKLAIYKATNLVPTSTFLLHSD FEQVTCIKENKIVKLLLCQNYDPLLVGQHVLRWIDKKCGKKNTLWFYGPPSTGKTNLAMAIAKTVPVYGM VNWNNENFPFNDVAGKSLVVWDEGIIKSTIVEAAKAILGGQPTRVDQKMRGSVAVPGVPVVITSNGDITF VVSGNTTTTVHAKALKERMVKLNFTIRCSPDMGLLTEADVQQWLTWCNAQSWSHYENWAINYTFDFPGIN ADALHPDLQTTPIVPDTSISSSGGESSEELSESSFFNLITPGAWNSETPRSSTPVPGTSSGESFVGSPVS SEVVAASWEEAFYTPLADQFRELLVGVDFVWDGVRGLPVCCVEHINNSGGGLGLCPHCINVGAWYNGWKF REFTPDLVRCSCHVGASNPFSVLTCKKCAYLSGLQSFVDYE TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_004295 |
ORF Size | 2013 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NC_004295.1, NP_694863 |
RefSeq ORF | 2013 bp |
Locus ID | 955445 |
MW | 74.0 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
VC100781 | Myc-DDK-tagged ORF clone of viral ORF for 75-kDa protein [Human erythrovirus V9], codon optimized for human cell expression, NP_694864 |
CNY 3,800.00 |
|
VC100782 | Myc-DDK-tagged ORF clone of viral ORF for VP1 protein [Human erythrovirus V9], codon optimized for human cell expression, NP_694865 |
CNY 11,880.00 |
|
VC100783 | Myc-DDK-tagged ORF clone of viral ORF for X protein [Human erythrovirus V9], codon optimized for human cell expression, NP_694866 |
CNY 3,800.00 |
|
VC100784 | Myc-DDK-tagged ORF clone of viral ORF for VP2 protein [Human erythrovirus V9], codon optimized for human cell expression, NP_694867 |
CNY 6,650.00 |
|
VC100785 | Myc-DDK-tagged ORF clone of viral ORF for 11-kDa protein [Human erythrovirus V9], codon optimized for human cell expression, NP_694868 |
CNY 3,800.00 |