VP2 (NC_004295) Virus Tagged ORF Clone

CAT#: VC100784

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for VP2 protein [Human erythrovirus V9], codon optimized for human cell expression, NP_694867



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CNY 6,650.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100784 represents NCBI reference of NP_694867 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCTCAGTGAACAGCGCCGAAGCCAGTACCGGAGCTGGAGGAGGAGGATCAAACCCGACTAAGTCTA
TGTGGAGTGAAGGTGCAACCTTCACCGCCAATTCCGTCACCTGTACCTTTAGCAGGCAGTTCCTGATTCC
CTACGACCCAGAACATCATTATAAGGTGTTCTCACCGGCGGCCAGTAGCTGTCATAATGCTTCCGGTAAG
GAAGCAAAGGTGTGCACTATCAGCCCAATTATGGGCTATAGTACGCCATGGCGCTACCTCGACTTCAACG
CCTTGAATCTGTTCTTTAGTCCACTGGAGTTTCAGCACCTGATCGAGAATTACGGCAGCATCGCTCCTGA
CGCACTGACAGTAACGATTAGTGAGATCGCCGTCAAGGACGTTACGGATAAGACGGGCGGAGGTGTCCAG
GTCACCGACAGTACAACGGGGAGACTGTGTATGCTTGTAGATCACGAATATAAATATCCCTATGTGCTGG
GGCAAGGTCAGGATACATTGGCCCCTGAGTTGCCTATTTGGGTATATTTCCCACCCCAGTATGCTTACCT
GACGGTGGGCGAGGTCAATACGCAGGGAATCAGCGGAGATTCTAAGAAACTTGCCTCAGAAGAATCAGCC
TTTTACGTTCTCGAGCACAGCTCCTTCGAGCTCCTGGGTACCGGTGGCAGTGCGACAATGTCTTACAAAT
TCCCTGCTGTGCCCCCTGAGAACTTGGAGGGCTGTTCCCAGCATTTTTACGAGATGTACAACCCTCTGTA
CGGCAGTCGATTGGGCGTTCCAGATACACTGGGTGGCGATCCTAAATTTCGCAGCCTTACCCATGAAGAC
CATGCCATTCAACCGCAGAACTTTATGCCGGGGCCTCTCATTAATTCCGTTTCAACAAAAGAGGGGGACA
ATTCCAACACTGGGGCCGGGAAGGCGCTGACAGGCCTGAGCACAGGGACTTCTCAAAATACCAGGATCAG
CCTGCGGCCAGGGCCTGTGAGTCAGCCCTATCACCACTGGGATACCGATAAATATGTCACCGGCATCAAC
GCCATTTCACACGGACAAACAACATACGGCAATGCCGAGGACAAGGAGTACCAACAGGGAGTGGGACGAT
TTCCAAATGAGAAGGAACAACTGAAGCAGCTGCAGGGCCTGAATATGCACACATATTTTCCAAACAAGGG
TACACAACAGTACACCGACCAGATTGAGCGGCCTCTGATGGTGGGATCTGTGTGGAACAGACGGGCGTTG
CACTACGAGTCCCAGCTGTGGTCTAAGATCCCGAATCTCGATGATTCATTCAAAACCCAGTTCGCCGCCC
TCGGCGGATGGGGCCTGCATCAGCCCCCACCCCAGATATTCCTGAAGATCCTGCCGCAGTCTGGGCCGAT
TGGAGGGATCAAAAGTATGGGAATTACTACTCTGGTTCAGTACGCAGTGGGTATCATGACTGTAACAATG
ACGTTCAAACTGGGTCCGCGCAAGGCTACTGGTAGGTGGAACCCACAGCCCGGAGTATACCCACCGCACG
CCGCTGGCCACCTGCCTTATGTGCTGTACGATCCTACCGCAACAGACGCTAAGCAGCACCACCGCCATGG
TTACGAAAAACCGGAGGAGCTGTGGACCGCTAAATCCCGCGTGCATCCGTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100784 representing NP_694867
Red=Cloning sites Green=Tags

MTSVNSAEASTGAGGGGSNPTKSMWSEGATFTANSVTCTFSRQFLIPYDPEHHYKVFSPAASSCHNASGK
EAKVCTISPIMGYSTPWRYLDFNALNLFFSPLEFQHLIENYGSIAPDALTVTISEIAVKDVTDKTGGGVQ
VTDSTTGRLCMLVDHEYKYPYVLGQGQDTLAPELPIWVYFPPQYAYLTVGEVNTQGISGDSKKLASEESA
FYVLEHSSFELLGTGGSATMSYKFPAVPPENLEGCSQHFYEMYNPLYGSRLGVPDTLGGDPKFRSLTHED
HAIQPQNFMPGPLINSVSTKEGDNSNTGAGKALTGLSTGTSQNTRISLRPGPVSQPYHHWDTDKYVTGIN
AISHGQTTYGNAEDKEYQQGVGRFPNEKEQLKQLQGLNMHTYFPNKGTQQYTDQIERPLMVGSVWNRRAL
HYESQLWSKIPNLDDSFKTQFAALGGWGLHQPPPQIFLKILPQSGPIGGIKSMGITTLVQYAVGIMTVTM
TFKLGPRKATGRWNPQPGVYPPHAAGHLPYVLYDPTATDAKQHHRHGYEKPEELWTAKSRVHPL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_004295
ORF Size 1662 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_004295.1, NP_694867
RefSeq ORF 1662 bp
Locus ID 955442
MW 60.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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