UL141 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101307

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein UL141 [Human herpesvirus 5], codon optimized for human cell expression, YP_081575



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CNY 3,656.00

CNY 4,090.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>VC101307 representing YP_081575.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTGCCGGCGGGAGTCCCTGCGGACACTGCCTTGGCTGTTCTGGGTGCTCCTGAGCTGCCCCAGACTG
CTGGAGTACTCCAGCAGCTCCTTCCCCTTTGCCACCGCCGACATCGCCGAAAAGATGTGGGCCGAGAAC
TACGAGACCACCAGCCCAGCCCCCGTCCTGGTCGCTGAGGGCGAGCAGGTCACCATCCCCTGCACCGTC
ATGACCCACTCCTGGCCCATGGTGAGCATCCGGGCCCGGTTTTGCCGGAGCCACGACGGCAGCGACGAG
CTCATCCTGGACGCCGTCAAGGGGCACCGGCTGATGAACGGCCTCCAGTACCGGCTCCCCTACGCCACA
TGGAATTTCAGCCAGCTGCACCTGGGCCAGATCTTCAGCCTGACCTTCAACGTGAGCACCGATACCGCC
GGCATGTACGAGTGCGTGCTGAGAAACTACTCCCACGGCCTGATCATGCAACGGTTCGTCATCCTCACC
CAGCTCGAAACCCTCAGCCGGCCCGACGAGCCCTGCTGCACACCCGCCCTCGGACGGTACAGCCTGGGC
GACCAAATCTGGTCCCCCACCCCCTGGCGGCTGCGGAACCACGACTGCGGAATGTACCGGGGGTTTCAG
CGGAACTATTTTTACATCGGCCGGGCCGACGCCGAGGACTGCTGGAAGCCCGCTTGCCCTGACGAGGAG
CCCGACAGATGTTGGACCGTGATCCAGCGGTACCGGCTGCCCGGCGATTGTTACCGGTCCCAGCCCCAC
CCCCCCAAGTTCCTGCCCGTCACCCCCGCCCCCCCCGCCGACATCGACACCGGCATGTCCCCCTGGGCC
ACCCGGGGCATCGCCGCCTTCCTGGGCTTCTGGAGCATTTTCACCGTGTGCTTCCTGTGCTACCTCTGC
TACCTGCAGTGCTGCGGGCGGTGGTGCCCTACCCCAGGCCGGGGACGGAGAGGCGGCGAAGGCTACAGA
AGACTGCCTACTTACGACAGCTACCCCGGCGTCAAGAAAATGAAAAGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>VC101307 representing YP_081575
Blue=ORF Red=Cloning site Green=Tag(s)

MCRRESLRTLPWLFWVLLSCPRLLEYSSSSFPFATADIAEKMWAENYETTSPAPVLVAEGEQVTIPCTV
MTHSWPMVSIRARFCRSHDGSDELILDAVKGHRLMNGLQYRLPYATWNFSQLHLGQIFSLTFNVSTDTA
GMYECVLRNYSHGLIMQRFVILTQLETLSRPDEPCCTPALGRYSLGDQIWSPTPWRLRNHDCGMYRGFQ
RNYFYIGRADAEDCWKPACPDEEPDRCWTVIQRYRLPGDCYRSQPHPPKFLPVTPAPPADIDTGMSPWA
TRGIAAFLGFWSIFTVCFLCYLCYLQCCGRWCPTPGRGRRGGEGYRRLPTYDSYPGVKKMKR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NC_006273
ORF Size 1014 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq YP_081575
RefSeq ORF 1014 bp
Locus ID 3077418
MW 38.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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