E1 (NC_001357) Virus Tagged ORF Clone

CAT＃: VC101805

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040312

Datasheet

SDS

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CNY 7,890.00

货期*

4周

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

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CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

TA592569

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 600.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA50011-100

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

CNY 1,050.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC101805 represents NCBI reference of NP_040312 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATCCCGAGGGCACTGATGGCGAGGGCACAGGATGTAATGGTTGGTTCTATGTGCAAGCCATCG TGGATAAAAAAACTGGAGATGTTATTTCTGACGACGAAGATGAGAATGCCACCGACACTGGCAGTGATAT GGTCGATTTTATAGACACCCAGGGGACATTCTGTGAACAAGCTGAGCTGGAGACTGCACAGGCTCTGTTC CATGCCCAGGAGGTGCACAATGATGCCCAAGTACTGCACGTCCTTAAAAGAAAGTTCGCGGGGGGCAGCA CGGAAAATAGTCCCCTCGGCGAGAGACTGGAAGTCGACACCGAACTCTCCCCCAGGCTCCAGGAGATCTC CCTTAATTCTGGACAGAAGAAGGCAAAACGGCGACTGTTCACAATTTCCGACTCCGGCTATGGATGTAGC GAGGTCGAGGCTACCCAGATCCAGGTTACAACCAATGGAGAGCACGGCGGAAACGTGTGCTCCGGAGGGT CTACCGAGGCCATAGATAACGGCGGGACAGAGGGCAACAATTCCTCCGTCGACGGCACCTCAGACAACTC AAACATCGAGAACGTGAATCCACAGTGCACGATTGCTCAACTGAAAGACCTGCTGAAGGTTAATAACAAG CAGGGCGCTATGCTCGCAGTGTTTAAGGACACCTATGGACTTAGCTTTACTGATCTGGTTCGAAATTTCA AATCCGACAAGACCACTTGCACAGACTGGGTTACGGCTATTTTTGGCGTTAACCCGACAATTGCCGAAGG GTTCAAAACTCTTATCCAGCCGTTCATTCTGTACGCACACATACAATGCCTCGATTGCAAGTGGGGAGTG CTGATCCTGGCACTTCTGAGGTACAAGTGTGGAAAGTCTAGGCTGACTGTAGCTAAAGGCTTGAGCACAC TGTTGCACGTGCCAGAGACATGCATGCTGATCCAGCCACCTAAACTCCGGAGCTCCGTCGCCGCTCTGTA TTGGTACCGCACTGGAATCAGTAACATCTCTGAGGTGATGGGAGACACCCCGGAGTGGATTCAGAGACTC ACAATCATACAGCACGGCATTGACGACTCTAATTTTGACTTGAGCGAAATGGTGCAGTGGGCCTTTGACA ATGAACTGACCGATGAATCCGATATGGCCTTTGAATATGCACTGCTCGCAGATAGTAATTCTAACGCCGC CGCATTCCTGAAATCTAACTGTCAGGCCAAGTATCTCAAAGACTGTGCCACTATGTGCAAGCATTACCGG CGCGCCCAAAAGCGGCAGATGAACATGTCACAGTGGATCAGGTTCCGGTGTTCCAAGATAGACGAAGGAG GCGATTGGAGGCCGATCGTGCAGTTCCTCCGGTACCAACAAATCGAGTTTATAACGTTTTTGGGTGCCTT GAAGTCATTTCTCAAAGGCACCCCAAAGAAGAACTGTCTGGTTTTCTGCGGACCGGCGAACACCGGCAAA TCTTACTTCGGCATGTCATTCATCCATTTCATTCAAGGAGCTGTTATTAGTTTTGTGAACTCCACGTCCC ACTTCTGGCTCGAGCCTCTGACCGACACTAAAGTCGCTATGCTGGACGACGCGACAACCACATGCTGGAC TTATTTTGACACATACATGCGGAATGCCCTCGATGGCAACCCCATAAGCATCGATAGAAAGCACAAGCCT TTGATTCAGCTTAAGTGTCCCCCCATCCTGCTGACAACCAACATTCACCCAGCTAAGGATAACAGGTGGC CGTATCTGGAGAGCCGCATCACAGTATTTGAGTTTCCCAACGCTTTCCCTTTTGACAAGAACGGAAACCC TGTTTACGAGATCAACGACAAAAACTGGAAATGCTTCTTCGAGCGCACCTGGTCTCGATTGGATTTGCAC GAGGAGGAGGAAGACGCTGACACTGAGGGCAACCCATTCGGCACCTTCAAGCTGCGGGCTGGCCAGAACC ATCGCCCGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC101805 representing NP_040312 Red=Cloning sites Green=Tags MADPEGTDGEGTGCNGWFYVQAIVDKKTGDVISDDEDENATDTGSDMVDFIDTQGTFCEQAELETAQALF HAQEVHNDAQVLHVLKRKFAGGSTENSPLGERLEVDTELSPRLQEISLNSGQKKAKRRLFTISDSGYGCS EVEATQIQVTTNGEHGGNVCSGGSTEAIDNGGTEGNNSSVDGTSDNSNIENVNPQCTIAQLKDLLKVNNK QGAMLAVFKDTYGLSFTDLVRNFKSDKTTCTDWVTAIFGVNPTIAEGFKTLIQPFILYAHIQCLDCKWGV LILALLRYKCGKSRLTVAKGLSTLLHVPETCMLIQPPKLRSSVAALYWYRTGISNISEVMGDTPEWIQRL TIIQHGIDDSNFDLSEMVQWAFDNELTDESDMAFEYALLADSNSNAAAFLKSNCQAKYLKDCATMCKHYR RAQKRQMNMSQWIRFRCSKIDEGGDWRPIVQFLRYQQIEFITFLGALKSFLKGTPKKNCLVFCGPANTGK SYFGMSFIHFIQGAVISFVNSTSHFWLEPLTDTKVAMLDDATTTCWTYFDTYMRNALDGNPISIDRKHKP LIQLKCPPILLTTNIHPAKDNRWPYLESRITVFEFPNAFPFDKNGNPVYEINDKNWKCFFERTWSRLDLH EEEEDADTEGNPFGTFKLRAGQNHRPL TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	NC_001357
ORF Size	1971 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_001357.1, NP_040312
RefSeq ORF	1971 bp
Locus ID	1489084
UniProt ID	P06463
MW	73.7 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC101803	Myc-DDK-tagged ORF clone of viral ORF for E6 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040310	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,320.00
VC101804	Myc-DDK-tagged ORF clone of viral ORF for E7 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040311	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC101806	Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040313	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,370.00
VC101807	Myc-DDK-tagged ORF clone of viral ORF for E4 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040314	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC101808	Myc-DDK-tagged ORF clone of viral ORF for E5 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040315	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC101809	Myc-DDK-tagged ORF clone of viral ORF for L2 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040316	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,610.00
VC101810	Myc-DDK-tagged ORF clone of viral ORF for L1 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040317	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,840.00

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