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E2 (NC_001357) Virus Tagged ORF Clone
CAT＃: VC101806
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040313
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CNY 4,370.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101806 represents NCBI reference of NP_040313 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGACGCCGAAGGAGACCCTGTCCGAGCGCCTGTCATGTGTGCAAGACAAGATCATCGACCATTATG AAAATGACAGCAAAGATATTGACAGCCAAATTCAATACTGGCAGCTTATCAGGTGGGAAAACGCTATCTT TTTTGCCGCCCGGGAACACGGCATTCAAACTCTGAACCACCAGGTTGTTCCTGCTTATAATATCTCCAAG TCCAAAGCTCATAAGGCAATAGAGCTGCAGATGGCACTGCAAGGACTGGCTCAGAGCAGATATAAAACCG AAGACTGGACACTGCAGGACACTTGTGAGGAGTTGTGGAATACTGAACCCACCCACTGCTTCAAAAAAGG CGGCCAAACTGTGCAGGTTTACTTCGACGGAAATAAGGACAACTGCATGACATATGTGGCATGGGATAGC GTCTACTATATGACAGACGCAGGAACATGGGATAAGACAGCAACCTGCGTCAGCCATCGCGGCCTGTATT ATGTGAAGGAGGGTTATAACACCTTTTACATCGAATTCAAGTCTGAATGTGAAAAATATGGCAACACCGG CACTTGGGAGGTGCACTTTGGAAATAACGTCATCGATTGCAATGACTCAATGTGTAGCACCAGTGACGAT ACAGTGTCTGCCACGCAGCTGGTCAAGCAGCTCCAGCACACCCCGAGCCCTTATAGTAGCACTGTGAGCG TAGGCACAGCGAAAACCTACGGGCAAACGTCCGCCGCAACCAGGCCAGGCCACTGCGGGCTGGCTGAAAA GCAGCACTGCGGACCAGTGAATCCTCTGTTGGGCGCCGCCACCCCCACTGGAAACAATAAGCGGAGAAAG CTGTGCTCCGGCAACACCACCCCCATCATCCATCTCAAAGGCGACAGAAACAGTCTTAAGTGCCTCCGGT ATCGCCTTCGAAAACACAGCGATCATTACAGAGATATTAGTTCAACTTGGCATTGGACCGGTGCGGGTAA CGAAAAGACAGGCATCCTGACTGTGACGTATCATTCCGAAACTCAAAGAACAAAGTTCCTGAATACCGTC GCTATTCCAGACAGCGTTCAGATTCTCGTTGGCTACATGACCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101806 representing NP_040313
Red=Cloning sites Green=Tags MQTPKETLSERLSCVQDKIIDHYENDSKDIDSQIQYWQLIRWENAIFFAAREHGIQTLNHQVVPAYNISK SKAHKAIELQMALQGLAQSRYKTEDWTLQDTCEELWNTEPTHCFKKGGQTVQVYFDGNKDNCMTYVAWDS VYYMTDAGTWDKTATCVSHRGLYYVKEGYNTFYIEFKSECEKYGNTGTWEVHFGNNVIDCNDSMCSTSDD TVSATQLVKQLQHTPSPYSSTVSVGTAKTYGQTSAATRPGHCGLAEKQHCGPVNPLLGAATPTGNNKRRK LCSGNTTPIIHLKGDRNSLKCLRYRLRKHSDHYRDISSTWHWTGAGNEKTGILTVTYHSETQRTKFLNTV AIPDSVQILVGYMTM TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001357 |
| ORF Size | 1095 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_001357.1, NP_040313 |
| RefSeq ORF | 1095 bp |
| Locus ID | 1489085 |
| UniProt ID | P06463 |
| MW | 41.4 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101803 | Myc-DDK-tagged ORF clone of viral ORF for E6 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040310 |
CNY 1,320.00 |
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| VC101804 | Myc-DDK-tagged ORF clone of viral ORF for E7 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040311 |
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| VC101805 | Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040312 |
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|
| VC101807 | Myc-DDK-tagged ORF clone of viral ORF for E4 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040314 |
CNY 3,800.00 |
|
| VC101808 | Myc-DDK-tagged ORF clone of viral ORF for E5 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040315 |
CNY 3,800.00 |
|
| VC101809 | Myc-DDK-tagged ORF clone of viral ORF for L2 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040316 |
CNY 5,610.00 |
|
| VC101810 | Myc-DDK-tagged ORF clone of viral ORF for L1 protein [Alphapapillomavirus 7], codon optimized for human cell expression, NP_040317 |
CNY 6,840.00 |
