E1 (NC_001586) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC101915 represents NCBI reference of NP_041803 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACGATACCGGAACTGAAGAAGGCCTCGGATGTTCCGGCTGGTTCTCAGTTGAAGCGATCGTAG AAAGAACCACGGAGAATACCATTTCCGACGACGAAGACGAGAATGTGGAGGACTCCGGATTGGACCTGGT GGACTTCGTGGACGACTCTCGCATCATACCTACCAACCAGCTTAAGGCACAGGCTCTCCTCAATAGGCAA CAGGCGCATGCCGATAAGGAGGCCGTGCAAGCGCTGAAAAGAAAGCTGTTGGGATCTCCTTACGAGTCTC CCGCGAGCGACCTCCAAGAAAGCATAAACAAAGAACTCTCTCCTAGACTCGGGGGCTTGCAATTGTGTCG CGGCTCTCAGGGCGCTAAACGGCGGCTGTTCCAGAGCCTGGAGAATAGAGACAGTGGGTATGGGTACTCC GAAGTCGAGATTCGGCAGGAACAGGTTGAGAATGGACACGGAGCCCCAGACGGCAGCATGGGCAATGGCG GGGGGATGGGATCTGTTCATGGCGTCCAGGAGAATCAGGAAATCGGCACCAATACGCCAACCACCCGGGT GGTGGAACTGCTGAAGTGTAAGAATCTGCAGGCCACCCTGCTCGGGAAATTTAAAGAGCTGTTCGGCCTC TCCTTTGGAGACCTTGTAAGACAGTTCAAAAGTGATAAATCCTCCTGCACAGACTGGGTCGTCGCCGCTT TTGGGGTCCACCATTCAATCGCTGAAGGTTTTAATACCCTCATTAAGGCAGAAGCCCTTTACACACATAT ACAGTGGCTGACCTGTACTTGGGGAATGGTGCTGCTGATGCTCATAAGGTTCAAGTGTGGAAAGAACAGA ACCACAGTGTCAAAAGGGATGTGTAAACTGCTCAACATCCCAGCTAATCAGCTCCTGATTGAACCGCCGA GGCTTCAGTCAGTCGCGGCTGCAATCTATTGGTTCCGAGCAGGAATCTCAAACGCTAGCGTCGTCACTGG TGAAACCCCAGAATGGATCCAACGACAAACCATCGTAGAACATTGTTTCGCCGACACCCAATTTAACCTG ACAGAGATGGTGCAGTGGGCATACGACAACGATCTCACAGAGGATTCAGATATCGCCTATGAGTATGCCC AGCGGGCTGACACCGACAGTAATGCTGCCGCTTTCCTGAAATCAAATTGCCAGGCCAAGTATGTCAAAGA TTGTGGCATTATGTGCCGCCACTATAAAAAGGCTCAAATGAAAAGAATGTCAATGCCACAGTGGATCAAA CACCGCTCCGAGAGAACTGGCGACAACGGGGACTGGCGCCCAATCGTGAAATTCATTCGGTACCAGGGCA TTGATTTTCTGACCTTTATGTCCGCCTTCAAGAAATTCCTCCACAATATCCCAAAGAAATCTTGTTTGGT GCTGATTGGGCCTCCCAACACCGGTAAATCTCAGTTCGGCATGAGCCTTGTGAAGTTTCTCGCCGGCACA GTTATCAGCTTCGTGAACTCCCACTCTCATTTTTGGCTGCAGCCTCTTGACTCCGCGAAAATAGCCATGC TGGACGATGCAACTCCCCCTTGCTGGACCTATTTGGACACCTATCTCAGGAACCTGCTTGATGGAAACCC TTGCAGCATCGATCGGAAACACAAAGCATTGACGGTAGTGAAGTGCCCTCCATTGATTATTACAAGCAAC ACCGATATCAGAACAGAAGACAGGTGGAAGTACCTGTATTCAAGGATCTCACTCTTCGAATTTCCCAATC CCTTTCCATTGGATAAAAACGGCAATCCAGTGTACGTGCTTAACGATGAGAATTGGAAAAGTTTCTTCCA GCGGTTGTGGAGCTCCCTTGAATTTCAAGAAAGTGAGGATGAGGAGGAGAACGGCGACACGGGGCAAACC TTCAGATGTGTCCCCGGCACCGTGGTGAGAACAGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC101915 representing NP_041803 Red=Cloning sites Green=Tags MADDTGTEEGLGCSGWFSVEAIVERTTENTISDDEDENVEDSGLDLVDFVDDSRIIPTNQLKAQALLNRQ QAHADKEAVQALKRKLLGSPYESPASDLQESINKELSPRLGGLQLCRGSQGAKRRLFQSLENRDSGYGYS EVEIRQEQVENGHGAPDGSMGNGGGMGSVHGVQENQEIGTNTPTTRVVELLKCKNLQATLLGKFKELFGL SFGDLVRQFKSDKSSCTDWVVAAFGVHHSIAEGFNTLIKAEALYTHIQWLTCTWGMVLLMLIRFKCGKNR TTVSKGMCKLLNIPANQLLIEPPRLQSVAAAIYWFRAGISNASVVTGETPEWIQRQTIVEHCFADTQFNL TEMVQWAYDNDLTEDSDIAYEYAQRADTDSNAAAFLKSNCQAKYVKDCGIMCRHYKKAQMKRMSMPQWIK HRSERTGDNGDWRPIVKFIRYQGIDFLTFMSAFKKFLHNIPKKSCLVLIGPPNTGKSQFGMSLVKFLAGT VISFVNSHSHFWLQPLDSAKIAMLDDATPPCWTYLDTYLRNLLDGNPCSIDRKHKALTVVKCPPLIITSN TDIRTEDRWKYLYSRISLFEFPNPFPLDKNGNPVYVLNDENWKSFFQRLWSSLEFQESEDEEENGDTGQT FRCVPGTVVRTV TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_001586
ORF Size	1926 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_001586.1, NP_041803
RefSeq ORF	1926 bp
Locus ID	1489422
MW	72.3 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.