E2 (NC_001586) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC101916 represents NCBI reference of NP_041804 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACCCTGGCTAAAAGACTGGATGCCTGTCAGGAGCAGCTGCTGGAACTGTATGAGGAAGATTCCA AGCACCTCGAAAAACATGTGCAGCACTGGAAGTGTCTCAGAATCGAGGCAGCCTTGCTCTTTAAGGCCAG AGAGATGGGATACGCGCAAGTCGGCCACCAAATTGTACCAGCACTCGAAATTAGCCGCGCGAAGGCACAC GTGGCAATCGAGATTCAACTTGCACTGGAGACACTGCTTCAGTCCACATTTGGTACCGAGCCTTGGACAC TCCAAGAGACGAGTTATGAAATGTGGCACGCAGAGCCAAAGAAATGTTTGAAGAAGCAGGGTAGGACAGT AGAAGTTGTCTTTGACGGGAATCCAGAAAACGCAATGCACTATACTGCTTGGACATTTATTTACGTCCAG ACACTGGATGGCACCTGGTGCAAAGTATACGGCCACGTTTGTTACGCTGGTCTGTATTACATTGTAGATA ACATGAAGCAGTTTTACTGCAACTTTAAAAATGAGGCAAAAAAGTACGGAGTAACCGGTCAGTGGGAAGT GCACGACGGAACACAGGTGATTGTGAGTCCCGCCTCTATATCCTCTACTACCACCACTGAAGCCGAAGTC TCTTCATCTGGTCTCACTGAACTCGTCCAGACTACCGACCTGTACAATACTACCCCCACTCCTACCACAA TCACTAGAAGCAATTGCGATCCCGATGGTACTGATGGCATCCTTTACAAAGACCCAACACCCACTACCCC CCCCCGAAAGCGCTACAGACAGTCTCTGCAGCCACCTACCAAACACCTGCAGCATTACGGCGTAACCAAC GTGCCAGTGGACCCTGGGTCTCAGCGCGTGACATCCGACAACAACAACAACCAGCGGCGGAACCCGTGCG GCAATCAGACAACACCAGTTATCCATCTGCAGGGCGACCCAAATTGTCTGAAGTGTCTTCGGTGGCGCTT GAAAAAGAACTGTTCCCATCTGTTCACGCAAGTCTCTTCCACCTGGCACCTCACCGAAAAAGACTACACC CGGGACAGCAAAGATGGGATCATCACCATTCACTACTACAACGAAGAGCAGAGGGACAAATTTCTGTCTA CTGTGAAACTCCCTCCCGGCATCAAGTCATGTATCGGCTATATGTCAATGCTCCAGTTCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC101916 representing NP_041804 Red=Cloning sites Green=Tags METLAKRLDACQEQLLELYEEDSKHLEKHVQHWKCLRIEAALLFKAREMGYAQVGHQIVPALEISRAKAH VAIEIQLALETLLQSTFGTEPWTLQETSYEMWHAEPKKCLKKQGRTVEVVFDGNPENAMHYTAWTFIYVQ TLDGTWCKVYGHVCYAGLYYIVDNMKQFYCNFKNEAKKYGVTGQWEVHDGTQVIVSPASISSTTTTEAEV SSSGLTELVQTTDLYNTTPTPTTITRSNCDPDGTDGILYKDPTPTTPPRKRYRQSLQPPTKHLQHYGVTN VPVDPGSQRVTSDNNNNQRRNPCGNQTTPVIHLQGDPNCLKCLRWRLKKNCSHLFTQVSSTWHLTEKDYT RDSKDGIITIHYYNEEQRDKFLSTVKLPPGIKSCIGYMSMLQFM TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_001586
ORF Size	1182 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_001586.1, NP_041804
RefSeq ORF	1182 bp
Locus ID	1489423
MW	45.0 kDa

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