E7 (NC_004104) Virus Tagged ORF Clone

CAT#: VC102022

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for putative transforming protein E7 [Human papillomavirus type 90], codon optimized for human cell expression, NP_671504



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CNY 3,800.00


货期*
4周

规格
    • 10 ug

Product images

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102022 represents NCBI reference of NP_671504 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATGGCCAGCGGCCCACTCTGAAGGACATTTTTCTCGAGCTTGACCAGCCCGACGCAGTCGATCTGC
ACTGTAATGAGCAACTTGCCTCTAGTGAAGAAGAGGATAATAGAGAGGACGGCGAGCAGCCAACCCAGCC
AACCGAACCAGCCCAGCAAGCCTATCGCGTAGTCACAAGCTGCTGTCGCTGCTGTTGCACCGTCCGCCTG
GTGGTTGAGAGTGGCCACGCTGAAATCAGGCAATTGCAGGAACTGTTGCTGGGAGCGCTGCACATAGTCT
GCCCTCTTTGCGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102022 representing NP_671504
Red=Cloning sites Green=Tags

MHGQRPTLKDIFLELDQPDAVDLHCNEQLASSEEEDNREDGEQPTQPTEPAQQAYRVVTSCCRCCCTVRL
VVESGHAEIRQLQELLLGALHIVCPLCA

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_004104
ORF Size 294 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_004104.1, NP_671504
RefSeq ORF 294 bp
Locus ID 951485
MW 10.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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