United States
Germany
Japan
United Kingdom
China
E1 (NC_004104) Virus Tagged ORF Clone
CAT＃: VC102023
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for putative replication protein E1 [Human papillomavirus type 90], codon optimized for human cell expression, NP_671505
View other clones from "Virus" (6)
Need custom modification / cloning service?
Get a free quote
CNY 7,790.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102023 represents NCBI reference of NP_671505 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACGGAGAGGGAACCAATAATGGTGATGATAGCGAAGAGGGCAGATCAGCCTGCGGGTGGTTCT GGGTCGAGGCTACTGTAGACCGGACAACAGGCCAGCAGATATCCAGTGATGAGGACGAGGAGGACGGGGA CAGCGGCCAGGACATGGTGGATTTCATCGACAACTCTCGCCACCCGGGCGACGGTCAGGAAGTGGCCCTG GAACTGTACCGCCAGCAAGAGGCGCAGGACGATGAGGCTTTTGTCCAAGCCTTGAAGCGCAAATATTTGG CTTCTCCCCTTGCAGCTGGCGTCTGCGTTGACAAAGACCTGAGCCCTAGACTGGATGCGATCACTCTTGG ACGGTCCAGCCAGAAGGCCAAGAGGAGGTTGTTTGAATTGCAAGATTCAGGATACGGCAACACACAGGTA GATATCGACGCAACGGAAGACCAGGTCGTGGGAGAATCCCGAGGGGGTGCTGGCACAGGAGGAGTGCAGG AGACAGAGGAGGAAGAACGACAAGGAGGGGACGGAGCTCCCGGAGCAGTGACCGCTCCCGCCCAGTCCGG CACGGACGCAATCCTGTCACTTTTGAAGACCTCTAACCTGAGGGCAACTCTTTTGGGCAAATTCAAAGAT ATTTATGGCCTGTCCTTTATGGAGCTGGCTAGGCAGTTCAAGAGTAACAGGACCACATGCCTGGATTGGG TAATTTGCGCCTTCGGGGTGTACTGCACAGTGGCAGAGGGCGTGAAAACACTCATTCAGCAGCACTGCCA ATACGCTCACATACAGGTCCAGACCTCCAATTGGGGGATGGTGGTGCTGATGTTGGTGAGATACAACTGT GCCAAAAATCGGGATACCGTCGCCAAATGTATGGGCAGGTTGTTGAATATCCCTGAGCAACATATGCTGA TCGAGCCCCCCAAAATTAGACACCCGGCCGCCGCTCTGTACTGGTACAAGGCCGGCATGGGTAATGCCTC TGAAATTATCGGAGAGACCCCAGAGTGGATCGTCAGACAAACCGTGGTTGGACACTCCCTGGAAGAGTGT CAGTTTCAACTCAGCGTTATGGTTCAGTGGGCCTACGATCACGACATCACAGATGAATCAATCCTCGCCT ATGAGTATGCTAAGCTGGCTGACGTCGATGGAAATGCAGCCGCCTTCCTCGCTAGTAATTGCCAAGCAAA GTACGTCAAAGATGCCTGTACAATGTGCAGGCACTATAAGCGGGCAGAGCAGGCTCAAATGACCATGAGC GAGTGGATAAGATTTAGGTCAGCCAAAATCTCTGAGGAGGGCGATTGGAGACCAATCGTCAGATTTTTGC GGCATCACGATATCGAATTTATTACTTTTGTGATTTCCCTCAAGAACTTTCTGAAAGGCATCCCCAAAAA ATGCTGCATAGTAATATACGGCCCGGCCGACACCGGGAAATCATATTTCTGTATGAGTCTGTTGCGGTTC CTTGGAGGCGTGGTGATCTCATACGCAAACTCAACTAGTCACTTTTGGCTCCAGCCCCTCTGTGACGCCA AAATTGGTCTCATCGACGACGTGACCCCTCAGTGTTGGAGCTATATAGACACCTACCTGAGAAACGCCCT GGACGGCAACCAGGTGTGCGTGGATCGAAAACATAGGCCTCTCCTGCAATTGAAGTGTCCTCCACTTTTG ATGACCACTAACACCAATCCACTGGAGGAGGACCGATGGAAATTCCTCAGGTCAAGACTGCAGCTTTTTA CTTTCCCCAATGCCTTCCCAGTGAACCAGAAGGGTGACCCCCTGTACACACTGAACGATGCCAACTGGAA GTGTTTCTTCCAGAGACTGTGGGCAAGGCTGGACCTGCATGAACAGGACGACCAAGAAGACAACGGAGAT ATTGGCCAACCATTCAAGTGCGTCCCTGGGGAGGTGGCTAGAACCGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102023 representing NP_671505
Red=Cloning sites Green=Tags MADGEGTNNGDDSEEGRSACGWFWVEATVDRTTGQQISSDEDEEDGDSGQDMVDFIDNSRHPGDGQEVAL ELYRQQEAQDDEAFVQALKRKYLASPLAAGVCVDKDLSPRLDAITLGRSSQKAKRRLFELQDSGYGNTQV DIDATEDQVVGESRGGAGTGGVQETEEEERQGGDGAPGAVTAPAQSGTDAILSLLKTSNLRATLLGKFKD IYGLSFMELARQFKSNRTTCLDWVICAFGVYCTVAEGVKTLIQQHCQYAHIQVQTSNWGMVVLMLVRYNC AKNRDTVAKCMGRLLNIPEQHMLIEPPKIRHPAAALYWYKAGMGNASEIIGETPEWIVRQTVVGHSLEEC QFQLSVMVQWAYDHDITDESILAYEYAKLADVDGNAAAFLASNCQAKYVKDACTMCRHYKRAEQAQMTMS EWIRFRSAKISEEGDWRPIVRFLRHHDIEFITFVISLKNFLKGIPKKCCIVIYGPADTGKSYFCMSLLRF LGGVVISYANSTSHFWLQPLCDAKIGLIDDVTPQCWSYIDTYLRNALDGNQVCVDRKHRPLLQLKCPPLL MTTNTNPLEEDRWKFLRSRLQLFTFPNAFPVNQKGDPLYTLNDANWKCFFQRLWARLDLHEQDDQEDNGD IGQPFKCVPGEVARTV TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_004104 |
| ORF Size | 1938 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_004104.1, NP_671505 |
| RefSeq ORF | 1938 bp |
| Locus ID | 951487 |
| MW | 72.6 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102021 | Myc-DDK-tagged ORF clone of viral ORF for putative transforming protein E6 [Human papillomavirus type 90], codon optimized for human cell expression, NP_671503 |
CNY 3,800.00 |
|
| VC102022 | Myc-DDK-tagged ORF clone of viral ORF for putative transforming protein E7 [Human papillomavirus type 90], codon optimized for human cell expression, NP_671504 |
CNY 3,800.00 |
|
| VC102024 | Myc-DDK-tagged ORF clone of viral ORF for putative regulatory protein E2 [Human papillomavirus type 90], codon optimized for human cell expression, NP_671506 |
CNY 4,560.00 |
|
| VC102025 | Myc-DDK-tagged ORF clone of viral ORF for putative E4 protein [Human papillomavirus type 90], codon optimized for human cell expression, NP_671507 |
CNY 3,800.00 |
|
| VC102026 | Myc-DDK-tagged ORF clone of viral ORF for putative minor capsid protein L2 [Human papillomavirus type 90], codon optimized for human cell expression, NP_671508 |
CNY 5,700.00 |
|
| VC102027 | Myc-DDK-tagged ORF clone of viral ORF for putative major capsid protein L1 [Human papillomavirus type 90], codon optimized for human cell expression, NP_671509 |
CNY 6,180.00 |
