E1 (NC_004500) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC102030 represents NCBI reference of NP_775307 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACAAAGGGATTGATCCCAAGGAGGGTTGCTCAACTTGGTGTTTGATCGAAGCTGACTGCTCCG ATGTTGACGGCGATTTCGAAAAGCTGTTCGATAAGGACACTGACTCCGATATTTCTGATCTGCTCGACGA CGGAGATTTGGGTGATGCTGAACTCGGAAACCCTCAGGAACTGCTCTGTCTTCAAGAGAGAGAAGAATCA GATCTCCAACTCCAGCAGCTTAAGAGGAAGTACTTTTCTCCTAAAGCAGTACTCCAGCTTTCCCCACAGT TGGAGTCCATCACGATATCACCACAGCGCAAGTCAAAAAGACGACTCTTTGAAGAACAGGACAGCGGCCT CGAGCTCTCTTTGACACATGAAGCTGAGGACAGCGTTGCAGAGGTCGAGGTACCCGGTAGTAAAGACGAC GTGCCAGAAACTGTGTCAGCCACGGCAGAGACTAAGGGGAGTCAGAACAAGGAACACTACAAGCAGCTCC TTCAATGCAGTAACGCAAGAGCTACACTGCTCTCAAAGTTCAAGGCAGCCTTCGGAGTCAGCTTCACCGA GTTGACACGACGCTATAAGAGCGACAACACCTGCTGCAGAGATTGGGCGATCATAGTGTATGGCCTCCAA GACGAGATTATTGAGGGATCTAAGCATCTCTTTCAGCAACACTGCGAGTATATTTGGCTTCACGTACTGT CTCCCATCAGCCTGTATCTCCTCTGCTTCAAGACAGGTAAATCTCGCAACACAGTGAAAAATTTGTTGAT GTCAATTTTGAATGTTGGCGACGCCCAGCTCATCGCCGACCCGCCTCAGATCAGATCAGTGGTCGCCGCG CTGTTCTGGTACAAGGAATCTATGAACAAGAACGTGTATACCCACGGGGAGTATCCCGAATGGATCGCCA ACCAGACCCTGCTGTCCCATCAAGAGTACGAAACCCAACAATTTGACCTTTCACGAATGATTCAGTGGGC ATACGACAATGAGTACACTGAGGATTCCGACATTGCTTATCATTACGCCAAATTGGCAGACGAGGACAGT AATGCCCGCGCCTTCTTGGCCCATAACAGCCAGGCCAAATTCGTCCGAGAATGCGGACAAATGGTGAGAC ATTACAAACGCGGCGAAATGAAGAATATGAGTATGAGCGCTTGGATATACACGCGGCTGAAATCAATAGA AGGACCAGGGCACTGGTCTGATATTGTGAAGTTTATCCGCTTTCAGCAGATTAATTTTATTATGTTTCTG GATGTCTTCAAACAGTTCTTGGCCTCCGTTCCCAAGAGGAATTGCCTCCTTATCTACGGGGCACCGGATT GTGGGAAATCCATGTTTTGCATGTCCCTGATTAAAGCTCTGAAGGGCAAGGTGATTTCATTCGTTAACGC CCGGTCACAGTTTTGGTTGTCCCCCCTGGTTGAAAGCAAAATCGCATTGCTGGATGACGCGACAGAATGC TGTTGGAATTATATAGATAACTACCTGCGGAACGGAATTGATGGAAATATGGTTAGCGTCGATTGTAAGC ACAAAAACCCCGTGCAAATTAGATTTCCCCCCCTGCTGATAACTTCAAACAATAATATAATGTCAGATCC AAAATACAAGTATCTGCACAGCCGGATCAAGGCATTCGAATTCGTGAATAAGTTTCCTTTTAAGGATGAC GGCAGCCCCCTGTTTGAGCTGACCGACCAGTCCTGGAAGAGCTTTTTCCAGCGGCTTTGGCGGCAGTTGG ATCTTAGCGATCAGGAGGACGAAGGCGAGGATGGCGGCAGTCAGCGCCCCTTTCAGTGTACCGCCAGACA GGTCAATGACAACCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC102030 representing NP_775307 Red=Cloning sites Green=Tags MADKGIDPKEGCSTWCLIEADCSDVDGDFEKLFDKDTDSDISDLLDDGDLGDAELGNPQELLCLQEREES DLQLQQLKRKYFSPKAVLQLSPQLESITISPQRKSKRRLFEEQDSGLELSLTHEAEDSVAEVEVPGSKDD VPETVSATAETKGSQNKEHYKQLLQCSNARATLLSKFKAAFGVSFTELTRRYKSDNTCCRDWAIIVYGLQ DEIIEGSKHLFQQHCEYIWLHVLSPISLYLLCFKTGKSRNTVKNLLMSILNVGDAQLIADPPQIRSVVAA LFWYKESMNKNVYTHGEYPEWIANQTLLSHQEYETQQFDLSRMIQWAYDNEYTEDSDIAYHYAKLADEDS NARAFLAHNSQAKFVRECGQMVRHYKRGEMKNMSMSAWIYTRLKSIEGPGHWSDIVKFIRFQQINFIMFL DVFKQFLASVPKRNCLLIYGAPDCGKSMFCMSLIKALKGKVISFVNARSQFWLSPLVESKIALLDDATEC CWNYIDNYLRNGIDGNMVSVDCKHKNPVQIRFPPLLITSNNNIMSDPKYKYLHSRIKAFEFVNKFPFKDD GSPLFELTDQSWKSFFQRLWRQLDLSDQEDEGEDGGSQRPFQCTARQVNDNL TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_004500
ORF Size	1836 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_004500.1, NP_775307
RefSeq ORF	1836 bp
Locus ID	956300
MW	70.3 kDa

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