E2 (NC_004500) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC102031 represents NCBI reference of NP_775308 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCTCTGTCCGACAGGTTCAATGCTCTTCAGGACAAGCTGATGACTATCTATGAGCGCGCCAGTG AGTCCCTCAAAGACCAAATCGAACACTGGAACCTTTTGAGGCAGGAGCAGGTTCTGTTCCACTACGCCCG ACAACGGGGTGTGCTCCGCCTTGGATATCAACCCGTGCCCGCACTGACTATCTCTGAGGCCAAGGCTAAG GAGGCCATTGCCATGGTCCTGCACCTCGAGGCATTGCAGAGATCTCCCTATAAAAACGAGAAGTGGACCC TTGTGAATACTAGTGTCGAAACTTTCCGCACCCCGCCAGAGAACTGCTTCAAAAAAGGGCCTAAGACCAT CGAAATCGTGTACGACGGGAATCCCGAAAACACAATGCTTTATACAATCTGGACCCACATTTATTTTGAG GACGATGAGGGCAACTGGCAGAAGACCGAGGGACACCTTGACTACCACGGTGCATACTTTATGGACGGGC TCAACAAACAGTACTACATCAGGTTTGCGCAGGATGCACGAAGATTTAGCGAGACCGGCGAATGGGAAGT GAAGTTCAATAACGAGATCCTGTTCGCACCAGTGACCTCTAGCACCAACTCTGAAGAAGAAAGGGACCGC CCAGCACCAGCAACCGACCCAGGTAGCCTGTCTCAAACCAGCGGTGGCCAGTCACCCGTCCCTACACAGA GGAAACAACCATCTAAGGGTAGATACGGCAGAAAGGATTCTGGAGCCACAACAGCTTCAAGGGGTATTCA GAGGCGACCTAAGGCGTCTCCCAGGAGGAGTAGAAGCCGATCAGGCAGTCGCTCCGGCAGTCAGGGCGAC GCTCGAACCCTGTTGACAGTAAGAAGAGGAGAGAGAGAACGGGGTCAGGGAAGAGGCCAGGGCAGCAGAG GCCGGGGGCGGTCAGGAGACCGCTCAAGATCCGGATCCCGCAGTAGATCAGGCAGCCGAAGGAGATCAAG ATCAAGGGGCCGCTCACGAAATGGACGCGAGAGGGGCAGGGCTGCATCTCGCGGGAGGCGCGGCTATTCA AACCGGAGGTCTCGATCCAAAAGCGTGGGCACTAGCGGCATCCCCCCCGAACAAGTAGGGAGCTCCTTGC AGGGGGTCGGCAGGCAGCATAGCGGCAGGCTGGCTAGACTTCTCGATGACGCTCGGGATCCCCCAGTTAT ACTGTTGAAGGGCCAGGCAAATACTCTGAAATGTTACAGATACAGAGCAAAGGAAAAGTATAAGGGGTAT TATGATTGTTTCTCAACCACATGGTCCTGGGTGTCTGCAGGGTCCAATGACCGGATCGGGCGGTCCAGGA TGATCATTTCCTTCACGAGCAAGTCCCAGAGGCAGATGTTTCTTAGCATTATGAAACTGCCCAAGGGCGT TGACTGGAGCCTGGGATGCTTCGATTCAATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC102031 representing NP_775308 Red=Cloning sites Green=Tags MEALSDRFNALQDKLMTIYERASESLKDQIEHWNLLRQEQVLFHYARQRGVLRLGYQPVPALTISEAKAK EAIAMVLHLEALQRSPYKNEKWTLVNTSVETFRTPPENCFKKGPKTIEIVYDGNPENTMLYTIWTHIYFE DDEGNWQKTEGHLDYHGAYFMDGLNKQYYIRFAQDARRFSETGEWEVKFNNEILFAPVTSSTNSEEERDR PAPATDPGSLSQTSGGQSPVPTQRKQPSKGRYGRKDSGATTASRGIQRRPKASPRRSRSRSGSRSGSQGD ARTLLTVRRGERERGQGRGQGSRGRGRSGDRSRSGSRSRSGSRRRSRSRGRSRNGRERGRAASRGRRGYS NRRSRSKSVGTSGIPPEQVGSSLQGVGRQHSGRLARLLDDARDPPVILLKGQANTLKCYRYRAKEKYKGY YDCFSTTWSWVSAGSNDRIGRSRMIISFTSKSQRQMFLSIMKLPKGVDWSLGCFDSI TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_004500
ORF Size	1431 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NC_004500.1, NP_775308
RefSeq ORF	1431 bp
Locus ID	956299
MW	54.1 kDa

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