F (NC_001781) Virus Tagged ORF Clone

CAT#: VC102101

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for fusion protein [Human respiratory syncytial virus], codon optimized for human cell expression, NP_056863



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CNY 6,408.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102101 represents NCBI reference of NP_056863 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGCTTATTCACAGGCTTAGCGCTATTTTTTTGACCTTGGCCATTAACGCCTTGTATCTGACAT
CCTCACAGAATATTACAGAAGAATTTTACCAGTCAACCTGCTCCGCGGTTAGTAGAGGGTATTTCTCTGC
TCTCCGGACTGGCTGGTATACATCAGTCATCACGATTGAGTTGTCTAATATCAAGGAGACAAAATGCAAT
GGCACAGACACCAAAGTGAAACTCATCAAGCAGGAGTTGGACAAGTACAAAAATGCTGTGACGGAACTTC
AACTGCTTATGCAGAATACACCCGCGGCAAACAATCGAGCAAGGAGGGAGGCTCCTCAGTACATGAATTA
TACAATTAATACAACCAAGAACCTTAATGTTTCAATCTCAAAAAAAAGAAAAAGGAGATTTCTCGGGTTC
CTGTTGGGCGTGGGCTCAGCCATTGCGAGCGGGATTGCTGTGTCCAAGGTTCTCCACCTGGAGGGAGAAG
TGAATAAGATTAAGAACGCGCTCCTGAGTACTAACAAGGCCGTCGTTTCTCTCAGCAATGGGGTGTCAGT
GCTGACGAGTAAAGTTCTGGACCTGAAAAACTATATCAACAATCAGCTGCTGCCCATCGTCAACCAGCAG
AGCTGCCGCATTAGCAACATTGAGACCGTAATCGAGTTCCAACAGAAGAACAGCCGGCTCCTGGAGATCA
ATCGAGAGTTCTCCGTTAATGCTGGTGTTACTACGCCCCTTAGCACTTACATGTTGACAAATAGCGAGCT
CCTCTCCCTGATAAACGACATGCCGATTACTAACGATCAGAAAAAGCTGATGTCTAGTAACGTCCAGATC
GTCAGACAGCAGTCTTATTCAATAATGAGCATAATTAAGGAAGAAGTATTGGCCTACGTGGTCCAACTGC
CAATCTACGGCGTGATCGATACCCCGTGCTGGAAACTTCACACTAGCCCTCTGTGTACGACAAATATCAA
AGAGGGATCCAACATCTGTCTTACACGAACCGACCGGGGCTGGTATTGCGACAACGCCGGTAGCGTCTCT
TTTTTCCCACAGGCCGATACCTGTAAGGTGCAGAGCAATAGGGTTTTTTGCGATACAATGAATTCCCTGA
CGCTGCCCTCCGAAGTAAGCCTGTGCAATACGGATATCTTCAATTCCAAATATGACTGTAAAATAATGAC
ATCAAAAACAGACATTTCCTCCAGCGTGATCACCAGCCTCGGCGCAATTGTTAGTTGTTACGGCAAAACG
AAATGCACGGCCTCCAATAAGAATAGAGGGATCATTAAAACCTTCTCAAACGGCTGTGATTACGTAAGCA
ATAAGGGCGTGGACACCGTGAGCGTTGGAAATACTCTCTACTACGTCAATAAACTGGAGGGTAAAAACCT
CTATGTCAAGGGCGAGCCTATCATCAATTACTATGATCCATTGGTGTTTCCTAGTGACGAGTTCGATGCA
TCCATTTCACAGGTTAACGAGAAGATTAATCAGTCCCTGGCATTTATCCGGCGGAGCGACGAGCTGCTGC
ACAATGTTAATACCGGTAAGTCCACTACTAACATCATGATAACCACAATTATCATTGTTATCATCGTCGT
CTTGCTGAGTCTTATTGCTATCGGCCTTCTCCTTTATTGCAAGGCCAAGAATACACCGGTGACTCTCTCT
AAGGACCAACTGAGTGGAATCAATAACATTGCATTCTCTAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102101 representing NP_056863
Red=Cloning sites Green=Tags

MELLIHRLSAIFLTLAINALYLTSSQNITEEFYQSTCSAVSRGYFSALRTGWYTSVITIELSNIKETKCN
GTDTKVKLIKQELDKYKNAVTELQLLMQNTPAANNRARREAPQYMNYTINTTKNLNVSISKKRKRRFLGF
LLGVGSAIASGIAVSKVLHLEGEVNKIKNALLSTNKAVVSLSNGVSVLTSKVLDLKNYINNQLLPIVNQQ
SCRISNIETVIEFQQKNSRLLEINREFSVNAGVTTPLSTYMLTNSELLSLINDMPITNDQKKLMSSNVQI
VRQQSYSIMSIIKEEVLAYVVQLPIYGVIDTPCWKLHTSPLCTTNIKEGSNICLTRTDRGWYCDNAGSVS
FFPQADTCKVQSNRVFCDTMNSLTLPSEVSLCNTDIFNSKYDCKIMTSKTDISSSVITSLGAIVSCYGKT
KCTASNKNRGIIKTFSNGCDYVSNKGVDTVSVGNTLYYVNKLEGKNLYVKGEPIINYYDPLVFPSDEFDA
SISQVNEKINQSLAFIRRSDELLHNVNTGKSTTNIMITTIIIVIIVVLLSLIAIGLLLYCKAKNTPVTLS
KDQLSGINNIAFSK

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001781
ORF Size 1722 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001781.1, NP_056863
RefSeq ORF 1722 bp
Locus ID 1489825
MW 63.7 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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