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protein 3B (NC_010810) Virus Tagged ORF Clone
CAT＃: VC102175
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for protein 3B [Human TMEV-like cardiovirus], codon optimized for human cell expression, YP_001950230
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CNY 3,800.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102175 represents NCBI reference of YP_001950230 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCTATTCTGGGGGAAAGAAAGGTGAGAAGCAAACTCTGCAAATCCTCGACGTTCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102175 representing YP_001950230
Red=Cloning sites Green=Tags MAAYSGGKKGEKQTLQILDVQ TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_010810 |
| ORF Size | 63 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_010810.1, YP_001950230 |
| RefSeq ORF | 63 bp |
| MW | 2.3 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102166 | Myc-DDK-tagged ORF clone of viral ORF for leader peptide [Human TMEV-like cardiovirus], codon optimized for human cell expression, YP_001950221 |
CNY 3,800.00 |
|
| VC102167 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein VP4 [Human TMEV-like cardiovirus], codon optimized for human cell expression, YP_001950222 |
CNY 3,800.00 |
|
| VC102168 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein VP2 [Human TMEV-like cardiovirus], codon optimized for human cell expression, YP_001950223 |
CNY 3,800.00 |
|
| VC102169 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein VP3 [Human TMEV-like cardiovirus], codon optimized for human cell expression, YP_001950224 |
CNY 3,800.00 |
|
| VC102170 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein VP1 [Human TMEV-like cardiovirus], codon optimized for human cell expression, YP_001950225 |
CNY 3,800.00 |
|
| VC102171 | Myc-DDK-tagged ORF clone of viral ORF for protein 2A [Human TMEV-like cardiovirus], codon optimized for human cell expression, YP_001950226 |
CNY 3,800.00 |
|
| VC102172 | Myc-DDK-tagged ORF clone of viral ORF for protein 2B [Human TMEV-like cardiovirus], codon optimized for human cell expression, YP_001950227 |
CNY 3,800.00 |
|
| VC102173 | Myc-DDK-tagged ORF clone of viral ORF for protein 2C [Human TMEV-like cardiovirus], codon optimized for human cell expression, YP_001950228 |
CNY 3,800.00 |
|
| VC102174 | Myc-DDK-tagged ORF clone of viral ORF for protein 3A [Human TMEV-like cardiovirus], codon optimized for human cell expression, YP_001950229 |
CNY 3,800.00 |
|
| VC102176 | Myc-DDK-tagged ORF clone of viral ORF for protein 3C [Human TMEV-like cardiovirus], codon optimized for human cell expression, YP_001950231 |
CNY 3,800.00 |
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