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NS5b (NC_009824) Virus Tagged ORF Clone
CAT＃: VC102209
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for NS5b [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491557
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CNY 7,130.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102209 represents NCBI reference of YP_001491557 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATGTCTTACTCCTGGACTGGCGCCCTGATTACACCCTGTTCTGCCGAAGAGGAGAAACTGCCGA TATCCCCGCTGAGCAATTCCCTGCTGCGGCACCATAACCTCGTGTATAGCACCTCCTCTCGAAGTGCCTC CCAGCGCCAGAAGAAAGTGACTTTCGATCGACTCCAGGTGCTTGACGACCACTACAAGACGGCACTCAAA GAAGTGAAGGAACGAGCCAGTAGGGTTAAGGCTCGCATGCTGACAATTGAAGAGGCATGTGCACTTGTTC CCCCGCACAGTGCCAGATCAAAATTTGGTTATTCAGCCAAAGACGTACGGAGCCTTTCCTCCAGGGCCAT AAACCAGATCAGGTCCGTTTGGGAAGATCTGCTCGAGGATACAACAACCCCCATCCCGACTACCATAATG GCCAAGAATGAGGTGTTCTGCGTTGATCCTGCTAAAGGCGGTAGGAAGCCTGCCAGGCTTATCGTTTATC CTGACTTGGGAGTTCGGGTGTGTGAGAAAAGAGCACTGTATGACGTGATCCAAAAGCTTTCTATCGAGAC CATGGGTCCCGCCTATGGGTTCCAGTATTCCCCTCAGCAAAGGGTCGAACGCCTTCTCAAGATGTGGACC TCCAAGAAGACCCCACTCGGATTCTCCTATGACACTCGCTGTTTTGACTCTACCGTGACCGAACAGGACA TCAGGGTGGAAGAAGAAATCTACCAGTGCTGCAATCTGGAACCAGAGGCACGGAAGGTGATTTCCTCACT GACAGAGAGACTTTATTGCGGAGGCCCAATGTTCAATAGCAAAGGCGCACAATGTGGATACCGGAGGTGT AGAGCGTCCGGAGTGCTGCCCACCAGCTTCGGAAACACTATAACATGTTACATTAAGGCTACAGCGGCCG CCAAAGCAGCTAATCTTAGGAACCCCGATTTCCTCGTGTGCGGTGACGATCTCGTGGTTGTGGCTGAGTC CGACGGCGTCGATGAAGATCGCGCCGCTTTGCGCGCATTCACTGAAGCTATGACGCGATACTCTGCTCCA CCTGGTGACGCTCCACAGGCTACATATGACCTCGAACTCATTACCTCCTGTTCCTCCAACGTATCAGTCG CCAGGGACGACAAAGGGAGAAGATACTACTATCTTACTAGGGACGCTACAACGCCATTGGCCCGCGCCGC CTGGGAGACCGCAAGACACACACCTGTCAATTCTTGGCTTGGCAACATAATCATGTACGCTCCTACCATT TGGGTTAGGATGGTGATGATGACCCACTTTTTTTCCATTCTGCAGTCACAGGAGATATTGGATCGCCCAC TTGACTTTGAGATGTACGGAGCCACATATAGCGTAACACCGCTGGATCTTCCGGCTATTATTGAACGGCT CCATGGCCTGTCCGCGTTTACTCTGCATAGTTACAGTCCTGTGGAGCTCAACAGGGTCGCGGGGACTCTC AGGAAGCTGGGGTGTCCACCCCTGAGAGCGTGGAGGCACCGCGCCAGAGCAGTAAGGGCCAAGCTGATCG CTCAGGGAGGCAAGGCCAAAATCTGCGGTCTCTACCTGTTTAATTGGGCCGTAAGGACGAAAACCAACCT CACACCTCTGCCTGCTGCTGGCCAGCTGGACCTGAGCAGCTGGTTCACAGTGGGAGTGGGAGGAAATGAC ATCTACCATTCCGTGTCTCGGGCGCGGACAAGGCATCTGCTCTTGTGTCTTCTGCTGCTTACTGTAGGTG TCGGAATCTTTCTGCTCCCAGCCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102209 representing YP_001491557
Red=Cloning sites Green=Tags MSMSYSWTGALITPCSAEEEKLPISPLSNSLLRHHNLVYSTSSRSASQRQKKVTFDRLQVLDDHYKTALK EVKERASRVKARMLTIEEACALVPPHSARSKFGYSAKDVRSLSSRAINQIRSVWEDLLEDTTTPIPTTIM AKNEVFCVDPAKGGRKPARLIVYPDLGVRVCEKRALYDVIQKLSIETMGPAYGFQYSPQQRVERLLKMWT SKKTPLGFSYDTRCFDSTVTEQDIRVEEEIYQCCNLEPEARKVISSLTERLYCGGPMFNSKGAQCGYRRC RASGVLPTSFGNTITCYIKATAAAKAANLRNPDFLVCGDDLVVVAESDGVDEDRAALRAFTEAMTRYSAP PGDAPQATYDLELITSCSSNVSVARDDKGRRYYYLTRDATTPLARAAWETARHTPVNSWLGNIIMYAPTI WVRMVMMTHFFSILQSQEILDRPLDFEMYGATYSVTPLDLPAIIERLHGLSAFTLHSYSPVELNRVAGTL RKLGCPPLRAWRHRARAVRAKLIAQGGKAKICGLYLFNWAVRTKTNLTPLPAAGQLDLSSWFTVGVGGND IYHSVSRARTRHLLLCLLLLTVGVGIFLLPAR TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_009824 |
| ORF Size | 1776 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_009824.1, YP_001491557 |
| RefSeq ORF | 1776 bp |
| MW | 66.2 kDa |
Documents
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| FAQs |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102201 | Myc-DDK-tagged ORF clone of viral ORF for C [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491549 |
CNY 3,800.00 |
|
| VC102202 | Myc-DDK-tagged ORF clone of viral ORF for E1 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491550 |
CNY 3,800.00 |
|
| VC102203 | Myc-DDK-tagged ORF clone of viral ORF for E2/NS1 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491551 |
CNY 4,180.00 |
|
| VC102204 | Myc-DDK-tagged ORF clone of viral ORF for NS2 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491552 |
CNY 3,800.00 |
|
| VC102205 | Myc-DDK-tagged ORF clone of viral ORF for NS3 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491553 |
CNY 7,280.00 |
|
| VC102206 | Myc-DDK-tagged ORF clone of viral ORF for NS4a [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491554 |
CNY 3,800.00 |
|
| VC102207 | Myc-DDK-tagged ORF clone of viral ORF for NS4b [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491555 |
CNY 3,800.00 |
|
| VC102208 | Myc-DDK-tagged ORF clone of viral ORF for NS5a [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491556 |
CNY 5,510.00 |
