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NS3 (NC_009824) Virus Tagged ORF Clone
CAT＃: VC102205
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for NS3 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491553
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CNY 7,280.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC102205 represents NCBI reference of YP_001491553 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAGAGAAGTCCTGCTTGGTCCAGCCGACGATTACAGGGAGATGGGGTGGCGCCTTCTGGCACCAA TTACGGCTTATGCTCAGCAAACACGCGGTCTCCTCGGTACAATTGTGACCAGTCTTACTGGTAGGGATAA GAATGTCGTAACAGGCGAGGTGCAGGTCTTGAGCACCGCCACCCAAACTTTCCTGGGAACAACAGTGGGT GGAGTCATTTGGACGGTGTACCACGGTGCAGGCTCAAGAACCCTTGCCGGCGCAAAACACCCGGCCCTTC AGATGTACACAAACGTGGACCAGGACCTTGTAGGCTGGCCGGCCCCACCGGGCGCTAAGTCACTCGAACC TTGCGCCTGTGGCTCATCTGATCTTTACTTGGTGACCAGAGACGCCGACGTTATTCCAGCAAGAAGGAGG GGAGATTCCACTGCATCCCTGCTGAGCCCCCGCCCATTGGCGTGCCTGAAAGGTTCCAGCGGCGGACCTG TTATGTGCCCCTCCGGCCACGTCGCGGGCATTTTCCGAGCAGCCGTATGTACAAGAGGCGTGGCAAAATC CCTCCAATTTATTCCTGTGGAGACGTTGTCTACACAGGCAAGGAGCCCCTCCTTTAGCGACAACAGCACG CCTCCAGCCGTGCCTCAGTCTTATCAGGTTGGCTATCTCCACGCCCCTACGGGGTCTGGCAAAAGTACGA AGGTCCCAGCAGCATACGTGGCACAGGGCTACAACGTTCTGGTTCTGAATCCTTCAGTCGCAGCTACCCT TGGCTTTGGATCTTTCATGTCCAGGGCCTATGGCATCGATCCTAACATTCGGACAGGTAACAGAACTGTC ACAACAGGAGCGAAGTTGACCTACTCTACCTACGGCAAGTTTCTTGCCGATGGGGGATGTAGTGGGGGTG CTTACGACGTCATTATTTGTGATGAATGTCACGCTCAGGATGCAACCTCTATTCTCGGAATCGGCACTGT CCTGGACCAGGCAGAGACCGCTGGAGTGCGGCTGACAGTGCTGGCCACAGCCACCCCTCCAGGTAGTATC ACAGTCCCACATTCCAACATTGAAGAGGTTGCGCTGGGCTCTGAGGGCGAGATACCATTTTATGGAAAGG CCATCCCAATAGCTCTCCTGAAAGGAGGTCGGCACCTTATTTTCTGCCACTCCAAAAAGAAATGCGATGA GATCGCAAGTAAGCTGCGGGGAATGGGACTCAACGCCGTCGCATACTACCGGGGCCTGGACGTGAGTGTA ATCCCCACCACTGGAGATGTGGTGGTATGCGCCACTGATGCCCTCATGACTGGCTTCACCGGCGACTTCG ATTCAGTGATCGATTGCAACGTAGCCGTCGAGCAGTACGTCGACTTCTCTCTGGATCCAACCTTCTCTAT CGAAACACGGACAGCCCCACAAGATGCCGTCTCCCGATCTCAAAGACGCGGCCGGACAGGGAGAGGCCGA CTCGGCACATACAGATATGTGGCCAGCGGAGAGCGACCCAGCGGCATGTTCGATTCAGTTGTGCTGTGCG AGTGTTATGACGCCGGATGTTCTTGGTATGATCTGCAGCCGGCTGAAACTACTGTTAGACTCCGGGCTTA TCTGTCCACCCCAGGGTTGCCCGTTTGTCAGGACCACCTCGATTTCTGGGAGTCTGTATTCACCGGCCTG ACTCACATCGACGCTCACTTTCTGTCCCAAACGAAGCAACAGGGTTTGAACTTCAGTTATCTGACTGCAT ACCAGGCTACAGTTTGTGCTCGGGCACAGGCGCCCCCACCTTCTTGGGATGAAATGTGGAAGTGCCTTGT ACGGCTTAAACCCACTCTCCATGGCCCGACCCCTTTGCTGTATCGCTTGGGTCCAGTTCAGAACGAAACT TGTCTGACTCATCCCATCACCAAGTATCTTATGGCCTGCATGAGCGCCGATCTGGAGGTCACTACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC102205 representing YP_001491553
Red=Cloning sites Green=Tags MGREVLLGPADDYREMGWRLLAPITAYAQQTRGLLGTIVTSLTGRDKNVVTGEVQVLSTATQTFLGTTVG GVIWTVYHGAGSRTLAGAKHPALQMYTNVDQDLVGWPAPPGAKSLEPCACGSSDLYLVTRDADVIPARRR GDSTASLLSPRPLACLKGSSGGPVMCPSGHVAGIFRAAVCTRGVAKSLQFIPVETLSTQARSPSFSDNST PPAVPQSYQVGYLHAPTGSGKSTKVPAAYVAQGYNVLVLNPSVAATLGFGSFMSRAYGIDPNIRTGNRTV TTGAKLTYSTYGKFLADGGCSGGAYDVIICDECHAQDATSILGIGTVLDQAETAGVRLTVLATATPPGSI TVPHSNIEEVALGSEGEIPFYGKAIPIALLKGGRHLIFCHSKKKCDEIASKLRGMGLNAVAYYRGLDVSV IPTTGDVVVCATDALMTGFTGDFDSVIDCNVAVEQYVDFSLDPTFSIETRTAPQDAVSRSQRRGRTGRGR LGTYRYVASGERPSGMFDSVVLCECYDAGCSWYDLQPAETTVRLRAYLSTPGLPVCQDHLDFWESVFTGL THIDAHFLSQTKQQGLNFSYLTAYQATVCARAQAPPPSWDEMWKCLVRLKPTLHGPTPLLYRLGPVQNET CLTHPITKYLMACMSADLEVTT TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_009824 |
| ORF Size | 1956 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_009824.1, YP_001491553 |
| RefSeq ORF | 1956 bp |
| MW | 69.5 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC102201 | Myc-DDK-tagged ORF clone of viral ORF for C [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491549 |
CNY 3,800.00 |
|
| VC102202 | Myc-DDK-tagged ORF clone of viral ORF for E1 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491550 |
CNY 3,800.00 |
|
| VC102203 | Myc-DDK-tagged ORF clone of viral ORF for E2/NS1 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491551 |
CNY 4,180.00 |
|
| VC102204 | Myc-DDK-tagged ORF clone of viral ORF for NS2 [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491552 |
CNY 3,800.00 |
|
| VC102206 | Myc-DDK-tagged ORF clone of viral ORF for NS4a [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491554 |
CNY 3,800.00 |
|
| VC102207 | Myc-DDK-tagged ORF clone of viral ORF for NS4b [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491555 |
CNY 3,800.00 |
|
| VC102208 | Myc-DDK-tagged ORF clone of viral ORF for NS5a [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491556 |
CNY 5,510.00 |
|
| VC102209 | Myc-DDK-tagged ORF clone of viral ORF for NS5b [Hepatitis C virus genotype 3], codon optimized for human cell expression, YP_001491557 |
CNY 7,130.00 |
