NS3 protease/helicase' (NC_004102) Virus Tagged ORF Clone

CAT＃: VC102220

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for NS3 protease/helicase' [Hepatitis C virus], codon optimized for human cell expression, NP_803144

Datasheet

SDS

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CNY 4,704.00

CNY 7,600.00

货期*

现货

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

TA592569

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 600.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA50011-100

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

CNY 1,050.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC102220 represents NCBI reference of NP_803144 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCTATTACAGCATACGCTCAACAAACGCGGGGGCTGCTCGGGTGCATAATTACAAGTCTGACTG GGCGGGATAAAAATCAGGTGGAAGGCGAAGTGCAGATAGTAAGTACCGCTACTCAAACTTTTCTCGCCAC ATGTATAAACGGCGTGTGCTGGACGGTTTACCACGGAGCAGGAACCAGGACTATTGCTTCACCAAAAGGT CCTGTTATTCAAATGTACACAAATGTCGATCAGGATCTCGTAGGTTGGCCCGCACCTCAAGGGAGCAGGT CACTGACCCCGTGTACCTGCGGGAGTTCCGATCTCTACCTTGTGACACGACACGCTGATGTAATTCCCGT CCGCAGGCGGGGGGATAGCAGAGGGAGTCTGCTGTCTCCAAGGCCGATCTCTTATTTGAAGGGCTCTTCC GGCGGCCCACTGCTGTGCCCAGCAGGCCATGCGGTAGGTCTGTTTCGAGCCGCCGTGTGTACCAGGGGCG TAGCGAAAGCTGTCGACTTCATACCTGTGGAGAATCTCGAGACGACTATGCGCTCACCAGTTTTCACCGA TAACAGCAGCCCGCCAGCCGTGCCGCAGTCCTTCCAAGTGGCTCACCTGCACGCCCCCACCGGATCCGGC AAGTCCACCAAAGTCCCCGCTGCTTACGCAGCCCAAGGCTACAAGGTCTTGGTACTGAATCCTTCAGTGG CTGCCACTCTCGGCTTTGGCGCCTACATGTCTAAGGCACACGGAGTTGATCCTAATATTCGAACCGGTGT ACGCACCATCACCACTGGCTCCCCTATCACTTATAGTACATACGGAAAATTTCTGGCCGACGGCGGATGT TCCGGGGGCGCCTATGATATCATAATATGCGACGAGTGCCACAGTACTGATGCTACCAGTATTCTCGGTA TCGGCACAGTCCTGGATCAGGCGGAAACAGCGGGTGCCAGACTGGTCGTTCTGGCCACGGCTACCCCACC TGGATCTGTGACAGTGTCTCACCCAAACATTGAGGAAGTGGCTCTGAGCACCACCGGTGAAATCCCGTTT TATGGTAAAGCTATCCCACTCGAGGTGATCAAAGGGGGCCGACACTTGATTTTTTGTCACAGTAAGAAAA AGTGTGATGAACTCGCGGCAAAACTGGTAGCTCTGGGGATTAATGCCGTGGCTTACTATAGAGGTCTGGA CGTGAGTGTGATTCCGACCAGCGGCGACGTCGTAGTTGTGAGTACAGATGCCCTCATGACTGGCTTTACA GGAGACTTTGATTCCGTTATTGATTGTAACACATGTGTGACCCAGACCGTGGACTTCAGCCTGGACCCCA CGTTCACAATTGAAACTACAACATTGCCCCAAGACGCCGTGTCCAGGACCCAGAGGAGAGGAAGGACAGG CCGCGGAAAACCTGGAATCTATAGATTCGTAGCCCCCGGGGAACGCCCATCCGGCATGTTTGATTCTTCC GTGTTGTGTGAGTGCTATGACGCTGGCTGCGCTTGGTACGAGCTTACTCCTGCAGAGACCACTGTCAGGT TGAGGGCCTACATGAATACACCAGGTTTGCCCGTATGCCAGGACCACCTGGAGTTTTGGGAAGGCGTCTT CACCGGCCTGACTCATATTGACGCTCATTTTCTGTCTCAGACAAAGCAATCTGGTGAGAATTTCCCTTAC CTGGTTGCCTATCAAGCAACGGTTTGTGCTAGGGCGCAGGCCCCCCCGCCCTCATGGGACCAAATGTGGA AATGTCTCATTAGGCTCAAGCCCACATTGCACGGACCCACACCTCTCCTGTATCGGCTCGGTGCAGTCCA GAACGAAGTTACACTGACCCACCCCATCACCAAGTATATCATGACCTGTATGAGCGCCGACCTGGAAGTG GTTACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC102220 representing NP_803144 Red=Cloning sites Green=Tags MAPITAYAQQTRGLLGCIITSLTGRDKNQVEGEVQIVSTATQTFLATCINGVCWTVYHGAGTRTIASPKG PVIQMYTNVDQDLVGWPAPQGSRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSS GGPLLCPAGHAVGLFRAAVCTRGVAKAVDFIPVENLETTMRSPVFTDNSSPPAVPQSFQVAHLHAPTGSG KSTKVPAAYAAQGYKVLVLNPSVAATLGFGAYMSKAHGVDPNIRTGVRTITTGSPITYSTYGKFLADGGC SGGAYDIIICDECHSTDATSILGIGTVLDQAETAGARLVVLATATPPGSVTVSHPNIEEVALSTTGEIPF YGKAIPLEVIKGGRHLIFCHSKKKCDELAAKLVALGINAVAYYRGLDVSVIPTSGDVVVVSTDALMTGFT GDFDSVIDCNTCVTQTVDFSLDPTFTIETTTLPQDAVSRTQRRGRTGRGKPGIYRFVAPGERPSGMFDSS VLCECYDAGCAWYELTPAETTVRLRAYMNTPGLPVCQDHLEFWEGVFTGLTHIDAHFLSQTKQSGENFPY LVAYQATVCARAQAPPPSWDQMWKCLIRLKPTLHGPTPLLYRLGAVQNEVTLTHPITKYIMTCMSADLEV VT TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	NC_004102
ORF Size	1896 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_004102.1, NP_803144
RefSeq ORF	1896 bp
MW	67.4 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC102214	Myc-DDK-tagged ORF clone of viral ORF for protein F [Hepatitis C virus], codon optimized for human cell expression, NP_803170	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102215	Myc-DDK-tagged ORF clone of viral ORF for core protein [Hepatitis C virus], codon optimized for human cell expression, NP_751919	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,600.00
VC102216	Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751920	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC102217	Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751921	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 4,370.00
VC102218	Myc-DDK-tagged ORF clone of viral ORF for p7 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751922	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102219	Myc-DDK-tagged ORF clone of viral ORF for NS2 protein [Hepatitis C virus], codon optimized for human cell expression, NP_751923	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC102221	Myc-DDK-tagged ORF clone of viral ORF for NS4A protein [Hepatitis C virus], codon optimized for human cell expression, NP_751925	10 ug 产品选项验证失败所有选项都是必需的	CNY 1,200.00 CNY 3,800.00
VC102222	Myc-DDK-tagged ORF clone of viral ORF for NS4B protein [Hepatitis C virus], codon optimized for human cell expression, NP_751926	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,400.00 CNY 3,800.00
VC102223	Myc-DDK-tagged ORF clone of viral ORF for NS5A protein [Hepatitis C virus], codon optimized for human cell expression, NP_751927	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 5,510.00

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