ORF033L (NC_003494) Virus Tagged ORF Clone

CAT#: VC102323

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for ORF033L [Infectious spleen and kidney necrosis virus], codon optimized for human cell expression, NP_612255



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CNY 3,800.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102323 represents NCBI reference of NP_612255 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCTGACCACACTGAAGAAGCAACAAACTGCTACTATGAAGCCCACAGCCTTGTTCGTCCTGCTGG
CAGCCGCGACCCTCCTGAAAATGTCTATGGCACACGAGACAGCATTTGAGCACATGCTGAAGAATCGCGC
TATGGACTGGCATAGCAAAGCAAAGAAAATATTTAACGAAGTCCACAACGACCCAGGTAAAAATTTCAAA
CCAGCTCAAGCCTTCATGTGCAAACCTTCCAGCTCCTGGTTTAGAACTTACATGACGACACATATGGGCC
ATAAAGGAAAGGCGATGGTGGTTGCTATGTGTACTCAGTATCGCGACTCACTTAAACAAACCCGACAAGC
CACCGCCGAGCACCTCAATAAGAAGTGTTATTATGTTGACGTGAAGGGTGCCGATTACAACGGATTTGCA
ATCCTGATGGACCTGCGGCACAGGTTCAGTATTCACAATGACAACGACGTGGCCGTTACATATAACGTGA
AGGGAGGGTATAAAGATGCTACAAAGAAAAGAGCTATCAGGGTGGACGCTTCAGGCATGAGGTATACTGA
TCAGCGGGTAACCATCCCACCACATGGAAGCACCTGGTATAAGGACGACTGGGGGCGGCTGGCTAATAGA
CCCGACGGATTTTGCTCCATGAGTCCCATGATGACACTGCCTTATGCATCCTGGCACAGACCCCGCTTCC
ACGTGCCCCAGTACACTCTGAATAAAAAGGTGTTCCCCTGTAAAGCCACGCTGATGTATGACGATGAGCA
CTATAATAGATTTCTGGAGACACACTTCGAAGATCGCCGAAAGATGTGTCTGTATGAACCCCACCAGACA
CTCCCAAGCAGCATCAAAAAGAAGGTGTCCTCCCTGTGGAACTGCAAAGAGAACATGGCAGTGTATATCA
GCTACTGCCCCCAAAGTTACTTGTGGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102323 representing NP_612255
Red=Cloning sites Green=Tags

MTLTTLKKQQTATMKPTALFVLLAAATLLKMSMAHETAFEHMLKNRAMDWHSKAKKIFNEVHNDPGKNFK
PAQAFMCKPSSSWFRTYMTTHMGHKGKAMVVAMCTQYRDSLKQTRQATAEHLNKKCYYVDVKGADYNGFA
ILMDLRHRFSIHNDNDVAVTYNVKGGYKDATKKRAIRVDASGMRYTDQRVTIPPHGSTWYKDDWGRLANR
PDGFCSMSPMMTLPYASWHRPRFHVPQYTLNKKVFPCKATLMYDDEHYNRFLETHFEDRRKMCLYEPHQT
LPSSIKKKVSSLWNCKENMAVYISYCPQSYLWQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_003494
ORF Size 939 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NC_003494.1, NP_612255
RefSeq ORF 939 bp
Locus ID 935306
MW 36.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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