CAMK2A Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CAMK2A
Locus ID	815
Kit Components	KN205202G1, CAMK2A gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GCTGGTACTCTTCCGTGAAG KN205202G2, CAMK2A gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GTGCCTCAGGAAGTCCTGGA KN205202-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TGAAGTTGCC AGGGTAACTG CATCCTGTCA TTCGTTCCTG GGGACCATCC GGAATGCGGC ACCCACTGGC TGTTACCATG GCAACTGCCT TTTTGCCCCA CTTAATCCCA TCCCGTCTGC TACAAGGGCC CCACAGTTGG AGGTGGGGGA GGTGGGAAGA GAAAAGATCA CTTGTGGACA AAGTTTGCTC TATTCCACCT CCTCCAGGCC CTCCTTGGGT CCATCACCCC AGGGGTGCTG GGTCCATCCC ACCCCCAGGC CCACACAGGC TTGCAGTATT GTGTGCGGTA TGGTCAGGGC GTCCGAGAGC AGGTTTCGCA GTGGAAGGCA GGCAGGTGTT GGGGAGGCAG TTACCGGGGC AACGGGAACA GGGCGTTTTG GAGGTGGTTG CCATGGGGAC CTGGATGCTG ACGAAGGCTC GCGAGGCTGT GAGCAGCCAC AGTGCCCTGC TCAGAAGCCC CGGGCTCGTC AGTCAAACCG GTTCTCTGTT TGCACTCGGC AGCACGGGCA GGCAAGTGGT CCCTAGGTTC GGGAGCAGAG CAGCAGCGCC TCAGTCCTGG TCCCCCAGTC CCAAGCCTCA CCTGCCTGCC CAGCGCCAGG GCGGGGAGGT CAGGGTCTCT AGTGGGGTGT GTGTGCCTGC AGTGCTTGGG GAGCATTCCT TGTACACAGA TGAAGGCAGG CTGCATGGGT TTGGGGGCAC CTGCCTGTGT ACAGAGTGTC CAGAGTCGGG CGTGGGTATG TTTGTGGGTG CACAGATGTG CCAAGCTAGC CAGGCCAGTG TGTATGTAGG GATGAAGGGT TGACAGTGTA TGCCAGTGCA CACACATACA CACAAAATGC ACACAAGTAC ACACAACCTG GATACACACA GATTCTTAGA GACTTAGGGT TAAAAAGGGA TTTTCCTAGT CAGCTGGGTC CAAAGCTGGA ATTCCTTCTC TAGTTTTGCT TGAGGTGGCC ATCCGCCCTC CACTTAGATA CCTCCAGTGA CAGAGAATTC ACCAGCTCCC GGTAGTTCTT CCTGACTTTA GACACCCACA GTGACTGTAC AGAGTGGCCA ACTTCTCCCT CCCTACCTCC CCTTTCTCAG CTCCTGCCAG GAGTGAGGGC GTCAGTACAG TAGGGGTTAA TATCTGAGCA TGACAGGCTT CCCGGGTGTT GGGAGGTGGG GAGAGGAAAT CAGGAAAGCT GTAGCGGGGA GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_015981, NM_171825, NM_001363989, NM_001363990, NM_001369025
Synonyms	CAMKA
Summary	The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2018]

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