CD3E Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CD3E
Locus ID	916
Kit Components	KN208276G1, CD3E gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GTGGTGTGTCTCCAGACCGC KN208276G2, CD3E gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CATCCTACTCACCTGATAAG KN208276-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet AAGATGGCTG CACCCTGCTG CTCCATGCCA GATCCACCAC ACAGAAAGCA AATGTTCAGT GCATCTCCCT CTTCCTGTCA GAGCTTATAG AGGAAGGAAG ACCCCGCAAT GTGGAGGCAT ATTGTATTAC AATTACTTTT AATGGCAAAA ACTGCAGTTA CTTTTGTGCC AACCTACTAC ATGGTCTGGA CAGCTAAATG TCATGTATTT TTCATGGCCC CTCCAGGTAT TGTCAGAGTC CTCTTGTTTG GCCTTCTAGG AAGGCTGTGG GACCCAGCTT TCTTCAACCA GTCCAGGTGG AGGCCTCTGC CTTGAACGTT TCCAAGTGAG GTAAAACCCG CAGGCCCAGA GGCCTCTCTA CTTCCTGTGT GGGGTTCAGA AACCCTCCTC CCCTCCCAGC CTCAGGTGCC TGCTTCAGAA AATGGTGAGT CTCTCTCTTA TAAAGCCCTC CTTTTTCATC CTAGCATTGG GAACAATGGC CCCAGGGTCC TTATCTCTAG CAGATGTTTT GAAAAAGTCA TCTGTTTTGC TTTTTTTCCA GAAGTAGTAA GTCTGCTGGC CTCCGCCATC TTAGTAAAGT AACAGTCCCA TGAAACAAAG ACAGCCTTAC CTGGCACTGC CTAGTGGCAC CAAGGAGCCT CATTTACCAG ATGTAAGGAA CTGTTTGTGC TATGTTAGGG TGAGGGATTA GAGCTGGGGA CTAAAGAAAA AGATAGGCCA CGGGTGCCTG GGAGAGCGTT CGGGGAGCAG GCAAAGAAGA GCAGTTGGGG TGATCATAGC TATTGTGAGC AGAGAGGTCT CGCTACCTCT AAGTACGAGC TCATTCCAAC TTACCCAGCC CTCCAGAACT AACCCAAAAG AGACTGGAAG AGCGAAGCTC CACTCCTTGT TTTGAAGAGA CCAGATACTT GCGTCCAAAC TCTGCACAGG GCATATATAG CAATTCACTA TCTTTGAGAC CATAAAACGC CTCGTAATTT TTAGTCCTTT TCAAGTGACC AACAACTTTC AGTTTATTTC ATTTTTTTGA AGCAAGATGG ATTATGAATT GATAAATAAC CAAGAGCATT TCTGTATCTC ATATGAGATA AATAATACCA AAAAAAGTTG CCATTTATTG TCAGATACTG TGTAAAGAAA AAATTATTTA GACGTGTTAA CTGGTTTAAT CCTACTTCTG CCTAGGAAGG AAGGTGTTAT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_000733
Synonyms	IMD18; T3E; TCRE
Summary	The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

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