CFTR Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CFTR
Locus ID	1080
Kit Components	KN216476G1, CFTR gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GAAGGTGGCCAACCGAGCTT KN216476G2, CFTR gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: ACAACGCTGGCCTTTTCCAG KN216476-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GAGGAGGAAG GCAGGCTCCG GGGAAGCTGG TGGCAGCGGG TCCTGGGTCT GGCGGACCCT GACGCGAAGG AGGGTCTAGG AAGCTCTCCG GGGAGCCGGT TCTCCCGCCG GTGGCTTCTT CTGTCCTCCA GCGTTGCCAA CTGGACCTAA AGAGAGGCCG CGACTGTCGC CCACCTGCGG GATGGGCCTG GTGCTGGGCG GTAAGGACAC GGACCTGGAA GGAGCGCGCG CGAGGGAGGG AGGCTGGGAG TCAGAATCGG GAAAGGGAGG TGCGGGGCGG CGAGGGAGCG AAGGAGGAGA GGAGGAAGGA GCGGGAGGGG TGCTGGCGGG GGTGCGTAGT GGGTGGAGAA AGCCGCTAGA GCAAATTTGG GGCCGGACCA GGCAGCACTC GGCTTTTAAC CTGGGCAGTG AAGGCGGGGG AAAGAGCAAA AGGAAGGGGT GGTGTGCGGA GTAGGGGTGG GTGGGGGGAA TTGGAAGCAA ATGACATCAC AGCAGGTCAG AGAAAAAGGG TTGAGCGGCA GGCACCCAGA GTAGTAGGTC TTTGGCATTA GGAGCTTGAG CCCAGACGGC CCTAGCAGGG ACCCCAGCGC CCGAGAGACC ATCATTCATT GTTTTGAAAG AAAATGTGGG TATTGTAGAA TAAAACAGAA AGCATTAAGA AGAGATGGAA GAATGAACTG AAGCTGATTG AATAGAGAGC CACATCTACT TGCAACTGAA AAGTTAGAAT CTCAAGACTC AAGTACGCTA CTATGCACTT GTTTTATTTC ATTTTTCTAA GAAACTAAAA ATACTTGTTA ATAAGTACCT AAGTATGGTT TATTGGTTTT CCCCCTTCAT GCCTTGGACA CTTGATTGTC TTCTTGGCAC ATACAGGTGC CATGCCTGCA TATAGTAAGT GCTCAGAAAA CATTTCTTGA CTGAATTCAG CCAACAAAAA TTTTGGGGTA GGTAGAAAAT ATATGCTTAA AGTATTTATT GTTATGAGAC TGGATATATC TAGTATTTGT CACAGGTAAA TGATTCTTCA AAAATTGAAA GCAAATTTGT TGAAATATTT ATTTTGAAAA AAGTTACTTC ACAAGCTATA AATTTTAAAA GCCATAGGAA TAGATACCGA AGTTATATCC AACTGACATT TAATAAATTG TATTCATAGC CTAATGTGAT GAGCCACAGA AGCTTGCAAA CTTTAATGAG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_000492
Synonyms	ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1; MRP7; TNR-CFTR
Summary	This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome. [provided by RefSeq, Aug 2017]

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