CSB (ERCC6) Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CSB
Locus ID	2074
Kit Components	KN219020G1, CSB gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GAGTGGGGGATTCCCTCATT KN219020G2, CSB gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GCTCCTGAGTTTGACTTGAG KN219020-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TCTATCCTCC CGCTTTGGCC TCCCAAAGTG CTGGGATTAC AGGACTGAGC CACGTGCCTG GCCTAATTAA TCTTCTGAGC TTCTGATTTG TTCAAAGCTC CTATTTACTG GATAGGTATT CAGCCTAGGA GTTTCTAGTC TCAGATTCAC ACAGAAGCTC TTTCCTAACA TCGCTGTTCC TATGCATTAC AGTTGATCTT GCCACTATAT TATAAATCAT TTGTGTCATA ATGAAATGAA GATGCTAACT TGGAAAACAG CAGCCAAATG ACTTCAGCTG TTTTGAAGAA TATTCCATGG CCATCACCCC AGGATTTTGG TGGCTGGCTT TCTTAGCTGG CTGCCTAAAT GTCCTTTGTT GATGATACTG CATTTCTTTT TCTGGTTAAT TTTTCTTATT TATTATCAGA ACTCAAGAAG ATACGTTGTC CCTTTTTAGT AAATTAATAC TTGGAAACTT GAATTTATGT AGTTGGAAGA TTATTACTTG ATCTTTTGAG AAATCAGTCT GCTCTAATTA TAAAATATTT AAGAACTACA ATGAATCCTA TATAACGAAA AGGCTTATAA ATTTTTTTCC TTTTAGGTAG TCTGTAGAGA AAGACTGTTT ACAGAGTCAA CCTGTCAGTA ATAATGAAGA AATGGCAATC AAGCAAGAAA GTGGTGGTGA TGGGGAGGTG GAGGAGTACC TCTCCTTTCG TTCTGTGGGT GACGGGCTGT CCACCTCTGC TGTGGGGTGC GCATCAGCAG CTCCGAGGAG AGGGCCAGCC CTGCTGCACA TCGACCGACA TCAGATCCAG GCAGTAGAGC CTAGCGCCCA GGCCCTTGAG CTGCAGGGTT TGGGTGTGGA CGTCTATGAC CAGGACGTGC TGGAACAGGG AGTGCTTCAG CAGGTGGACA ATGCCATCCA TGAGGCCAGC CGTGCCTCCC AGCTCGTTGA CGTGGAGAAG GAGTATCGGT CGGTCCTGGA TGACCTCACG TGAGTGCAGC CCATCTTCTT CCTTTCAAAG TGGTGGTAAA ACATGACAGG GATATTCAGG TAATGGAAAG CTCTAGTATT CCAGAAGTTT AGGAGATGTG TAGAACCTTT TATTTGGAGG ACAGAGTCAA TAAGGGCAGT CCTCAACCTC ATCTTTGAGT CCCAGCCCTT CCCCTTAGGA GTGGCATTGT ACATGGTAGT TAGAGTCTCT GTCCAGCTCG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_000124, NM_001277058, NM_001277059, NM_001346440
Synonyms	ARMD5; CKN2; COFS; COFS1; CSB; RAD26; UVSS1
Summary	This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The encoded protein has ATP-stimulated ATPase activity, interacts with several transcription and excision repair proteins, and may promote complex formation at DNA repair sites. Mutations in this gene are associated with Cockayne syndrome type B and cerebrooculofacioskeletal syndrome 1. Alternative splicing occurs between a splice site from exon 5 of this gene to the 3' splice site upstream of the open reading frame (ORF) of the adjacent gene, piggyback-derived-3 (GeneID:267004), which activates the alternative polyadenylation site downstream of the piggyback-derived-3 ORF. The resulting transcripts encode a fusion protein that shares sequence with the product of each individual gene. [provided by RefSeq, Mar 2016]

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