Wilms Tumor Protein (WT1) Human Gene Knockout Kit (CRISPR)

CAT#: KN220079LP

WT1 - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

  See Other Versions

CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


Rabbit monoclonal anti-WT1 Antibody, clone OTIR5F11
    • 100 ul

CNY 4,390.00


WT1 (Myc-DDK-tagged)-Human Wilms tumor 1 (WT1), transcript variant A
    • 10 ug

CNY 3,656.00
CNY 4,370.00

Specifications

Product Data
Format 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
Donor DNA Luciferase-Puro
Symbol Wilms Tumor Protein
Locus ID 7490
Kit Components

KN220079G1, Wilms Tumor Protein gRNA vector 1 in pCas-Guide CRISPR vector

KN220079G2, Wilms Tumor Protein gRNA vector 2 in pCas-Guide CRISPR vector

KN220079LP-D, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_000378, NM_001198551, NM_001198552, NM_024424, NM_024425, NM_024426, NM_001367854, NR_160306
Synonyms AWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT33
Summary This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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