RFXANK Human Gene Knockout Kit (CRISPR)

CAT#: KN220744

RFXANK - human gene knockout kit via CRISPR, HDR mediated




 HDR-mediated knockout kit validation

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CNY 12,260.00


货期*
6周

规格
    • 1 kit

Product images

经常一起买 (3)
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
    • 10 ug

CNY 3,710.00


RFXANK mouse monoclonal antibody, clone OTI3E7 (formerly 3E7)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


RFXANK (Myc-DDK-tagged)-Human regulatory factor X-associated ankyrin-containing protein (RFXANK), transcript variant 1
    • 10 ug

CNY 2,400.00
CNY 3,990.00

Specifications

Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA GFP-puro
Symbol RFXANK
Locus ID 8625
Kit Components

KN220744G1, RFXANK gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GTCTGGATGAGGTCTTCTGC

KN220744G2, RFXANK gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGACCCCTGCCTCAGAACTT

KN220744-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGGG TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG TTGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGC CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCAAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTACTATGGT TGCTTTGACG TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGGAG GCTACAGTCA GTGGAGAGGA CTTTCACTGA CTGACTGACT GGAAGACACA CCTTTTTTGA GACAGAGTCT TGCTCTGTCG CCCCAGCTAC AGTGCAGTGG TGCGATCTCT GCTCACCGCA ACCTCCACCT CCCGAGTTCA AGCAATTCTG CCTCAGCCTC CCGAGTAGCT GGGATCACAG GCGCCCGCCA CCGCGCTTGG CTAATTTTTG TATTTTTAGT ACAGATGGGG TTTCACCATG GTGGCCAGCC TGGTCTCGAA CTCCTGACCT CATGATCCAC CCTCCTCGGC CTCCCAAAGT GCTGGGATTA CAGCCGTGAG CCACTGTGCC CTGCCAGAGC CCAGGCTCTT AAACATTGCA CATTGTAGGT GCTTTGGTGG TGGGGAATGG TAGACCTCGA AAGCAGTTGG ACTCTGGTTT TCATTTCCCT GGCTCTGGTA ATTAACCTGG ACCTCAGTTT ACCCACCCTC ACAGTGCAAG GCTAGGTATG CAGTCGGTGC CTACTTTATT ATAACTGTTG ATAATTATTG TCATCTCTCC CCTTCTGACA CCTCCGCCAG CTTTCCCCAC TAGCATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAG AATTCCGATC ATATTCAATA ACCCTTAATA TAACTTCGTA TAATGTATGC TATACGAAGT TATTAGGTCT GAAGAGGAGT TTACGTCCAG CCAAGCTTAG GATCTCGACC TCGAAATTCT ACCGGGTAGG GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC GACTCCGTTC TTTGGTGGCC CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG GACGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA GCACCGCTGA GCAATGGAAG CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT CCTTCGCTTT CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG GGCTCAGGGG CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG CCCGGCATTC TGCACGCTTC AAAAGCGCAC GTCTGCCGCG CTGTTCTCCT CTTCCTCATC TCCGGGCCTT TCGACCTGCA TCCATCTAGA TCTCGAGCAG CTGAAGCTTA CCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCAC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGA TGATATCAGA TCCCCGGGAT GCAGAAATTG ATGATCTATT AAACAATAAA GATGTCCACT AAAATGGAAG TTTTTCCTGT CATACTTTGT TAAGAAGGGT GAGAACAGAG TACCTACATT TTGAATGGAA GGATTGGAGC TACGGGGGTG GGGGTGGGGT GGGATTAGAT AAATGCCTGC TCTTTACTGA AGGCTCTTTA CTATTGCTTT ATGATAATGT TTCATAGTTG GATATCATAA TTTAAACAAG CAAAACCAAA TTAAGGGCCA GCTCATTCCT CCCACTCATG ATCTATAGAT CTATAGATCT CTCGTGGGAT CATTGTTTTT CTCTTGATTC CCACTTTGTG GTTCTAAGTA CTGTGGTTTC CAAATGTGTC AGTTTCATAG CCTGAAGAAC GAGATCAGCA GCCTCTGTTC CACATACACT TCATTCTCAG TATTGTTTTG CCAAGTTCTA ATTCCATCAG AAGCTGGTCG AGATCCGGAA CCCTTAATAT AACTTCGTAT AATGTATGCT ATACGAAGTT ATTAGGTCCC TCGAAGAGGT TCACTAGGCG CGCCTGCAGA TGGCTCAGAC ACTGTGGTCC TCAGTCTCTT TCCCTGCACC CCTGAGCCTG TGAATCCTGA ACCGGATGCC AGTGTTTCCT CTCCACAGGG TAGGATACCT CCTCTGGGAT TAGCCCTCTG GGATTCCATG ATGATGGAAT GTCAGGCCTC ACATGGAACC TGTGTCTTGC TTTCGTTTTT GTTTTGTTTT GTTTTGTTGT TTTTTGAGAT GGAGTTTTGC TCTTGTTGCC CATGCTGGAG TGCAATGGCG TGATCTCAGC TCACTGCAAC CTCTGCCTCC TGGGTTCAAC TGATTCTCCT GTCTCAGCCT CCTGAGTAGC TGGGATTACA GGCTTCCGCC ACTACGCCCA GCTAATTTTT TGGGTATTTT TAGTAGAGAC GGGGTTTCAC CATGTTGGCC AGGCTGGTCT TGAACTCCTG ACCTCAGGTG ATCCGCCCAC CTCGGCCTCC CAAAGTGCTG GGATTACAGG CATGAGCCAC TGTGCCCGGC CTGTTTTTGT TTTTTGATAC AGGGTCTCGC TCTGTTACTA AGGCTGGAGT GCAGTGATGC AATCATAGTT CACTGCAGCC TCAACTTCCT GGGCTGAAGC CATCCTACCA GTCTTCACTG ACTGACTGAC TGGAAAGAGG AAGGGCTGGA AGAGGAAGGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATC

GE100003, scramble sequence in pCas-Guide vector

Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_001278727, NM_001278728, NM_003721, NM_134440, NM_001370233, NM_001370235, NM_001370237, NM_001370238, NM_001370234, NM_001370236
Synonyms ANKRA1; BLS; F14150_1; RFX-B
Summary Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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