Lgals1 Mouse Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Lgals1
Locus ID	16852
Kit Components	KN309240G1, Lgals1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AGAGGGTCCCGCACTCACAC KN309240G2, Lgals1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GACAATTGGGGACTTGCTTC KN309240-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet GGTGGGGCCT GTGCTTTGTG GTCCTCCTCA GTCCTCCCGC AGAAGCCCAC AGTGGGAGTC ACAGCGAGAG CCACACCCTG GCCTGCCAGG CGGCAGCCTC TTCAGAGAGC CTTGCTCTAA TTCTCTGCAG GCATCTATAC ACACATCCCC CTCCCCCACC AGCTGCGCTG GGACCTAGGG GCTTTCTCCC TGCTGGCCCC AGGCCCTATC CCGCCAACTC TTAGGTTTAA CTCATCCTTA AACTATTTTC CATTGGCTCC CCCAATCTCT GCCACAGCAC AAATCTTTTT TTAGTGGAAA GAGGGATGGA GAAGAATAAG AGGCGGCGCC AGGACCCTGA GGGAGGGGCT AGGAGGGGGG GTCTCCTCTC TGACTGGTCA CCTCTGCTCC AAAATTGACT TTATAATCCA TAGACTCCAC TTCTGGTGGC CCCCATCCTT GTCCTGACAT GCAATTGGCT GAACTCCCGG GGAGGGGCGG GACTCACCGG GTCTGATCCA GTTAAAAAGG TCGGAGCGGG CCTGGGGCCC GTCTCTCGGG TGGAGTCTTC TGACTGCTGG TGGAGCAGGT CTCAGGAATC TCTTCGCTTC AGCTTCAATC TGCCTTGGAC AGGTGTTTTC TCTGGTCCTC AGTGGCTACA TCTGTAAAAT GGGGGTGGCT GTCACTGTTC CCCCCTGAAA CCTTAAGCGT CTCTGGCTTA AGAATGTTCT GGACTTCTCG TAGTTCTGGC CGCATACCTG CCTTGCAAAG TCCAGTATTC TGCTGCTTTG CCGTTTTCTG AGAAGTGGGA GTGGGAAGGG TGTGGCCACC CGGGGAAAAG GGAGGCAGGG GGACATTTTG GCCAAGGCCA TTCCCTGTTC ACTCTTCCTC TCCACTCTAG AGTCTCCTGA CCCCTCCCCC GCTGCAGCTG TCCCCAGTCA CAGGGCCAGG GCTCAGTTGG TCACCCTCTG CTCCAGAAAA CTAAAAAAAA AAAAAAAAAA ACCCTCCCGG GCCTAAGATA GGCTCCAGGC TGGTCAGTGG AGGCGGGTCA GGGCTGGGCG GAGGAAACTG CATGCATTTG CACAGACAGG CAAGCCCTCA GGTATAGGGT TCAAATGTCA AAGAAACCCC TTGCCCAAAT GAACTCCTGT CAACTCACCC AGGTAGGTGC ACAGCAGTGC CCTGATATGT ACTGGGCTCT CTAGCGTGCT AAGTCCACAT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_008495
Synonyms	AA410090; Gal-1; Galbp; galectin-1; L-14.5; L14; Lect14
Summary	Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.[UniProtKB/Swiss-Prot Function]

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