Mettl4 Mouse Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	Mettl4
Locus ID	76781
Kit Components	KN310008G1, Mettl4 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TGATCCAGTAACCACCCAGG KN310008G2, Mettl4 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGCATCATTTGCCTCCTGGG KN310008-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TCCTATTGCT CTACAACTGA TAACGATTAG GTAGGGCAGG GATCTGAGTA ATGGAGTGAC TTCTGTGGCA ATAGTAGTTT GAAAACACAA CAGCTCTTTT TCCGTTACTT TCTGTGGCCC TCTCTAAATA GATTTATATC ATTTAAAAAA GGGTAAATTC TTATAGGTAA TTACTATCCT TTGACTTGAC AGGACACTCA CCTTTTAAGG ATTTAGTGAC TTGAATACTT GGACCATGTG GACTCGTGCC TTGATTCGAG GACCAAAACA GGACCACAGA GGACACCCGA AGAAAGGAGA ATCAAACTAA GAAGCGTCTT TGACTACATT GGCTGAAAAT TTTGAATGAC CAATTCTGAA ATTAAAAGAC ACTTAAAGTA TCGCATTTTT CCTTAGAGGT GATATGTACA CACTCACCTT GTATGTTTAA ATATAAAACC TTCTTGTGTG TACAAAGTAC ATCGTTGATA TACAGGACAT TAAGAACTTA ATTACAACAT TGGTATCAAC CCAGGACCCC CATACTGAGC TATACTCCCA GTCCTAGTTC TGATTCTTGA TTATCAGTTT TTTGTTTTTG TTTTTTGTTT TTTTGTGAGA AACTTTGCCA GCATCAAGAG TCTTTTTGTA GTAAAAACAA CCCTACTTCT TCTGTCTACA TGGATTCTCT TCAGCTGGAT CCCGGGTCTC CTTTTGGAGC TCCTGCTATG TGCTTTGCTC CTGACTTCAC TACTGTGTCG GGCAATGACG ATGAAGGAAG TTGTGAAGTG ATCACCGAAA AATATGTTTT CCGATCTGAG CTATTTAATG TCACCAAACC TTACATAGTT CCAGCTGTTC ACAAAGAGCG CCAACAAAGT AACAAAAACG AAAATCTAGT GACTGACTAT AAACAAGAGG TCTCTGTTTC TGTTGGGAAG GTAGGAAGTT TTAATTGTCT TTTTTTTTTT TTAACATGAA ACAAAAAATC TCATTAAGTA TCTCAAACAT CTTCCCATTT TCTTTTTTTT TAAAGGTTCT AAGATTTTTT TATTTTTTTA TAGTTTTTTT TTCCAATTTT TTTATTAGGT ATTTACTTCC TTTACATTTC AAATGCTATC CCGAAAGTTC CCTATGCCCC CCTACCCCCA CCTTCCCATT TTCTAAGTAT AAAAAAGAGT TAATAATGCA GACGGTGTTA GCATGTCCAA GAGCAGTTGT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_176917, NM_001357135, NM_001357136, NM_001357137, NM_001357138
Synonyms	2410198H06Rik; A730091E08Rik; AV296509; HsT661
Summary	Probable methyltransferase.[UniProtKB/Swiss-Prot Function]

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