NADK (NM_023018) Human Mass Spec Standard

Specifications

Product Data
Description	NADK MS Standard C13 and N15-labeled recombinant protein (NP_075394)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC200544
Predicted MW	49.2 kDa
Protein Sequence	Sequence Link (showhide) >RC200544 protein sequence Red=Cloning site Green=Tags(s) MEMEQEKMTMNKELSPDAAAYCCSACHGDETWSYNHPIRGRAKSRSLSASPALGSTKEFRRTRSLHGPCP VTTFGPKACVLQNPQTIMHIQDPASQRLTWNKSPKSVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEK KVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLG FLTPFSFENFQSQVTQVIEGNAAVVLRSRLKVRVVKELRGKKTAVHNGLGEKGSQAAGLDMDVGKQAMQY QVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICP HSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFES LAQCLHWNVRKKQAHFEEEEEEEEEG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_075394
RefSeq Size	3244
RefSeq ORF	1338
Synonyms	dJ283E3.1
Locus ID	65220
Cytogenetics	1p36.33
Summary	NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP, which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al., 2001 [PubMed 11594753]).[supplied by OMIM, Mar 2008]
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Nicotinate and nicotinamide metabolism

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