GLI1 (NM_005269) Human Mass Spec Standard

Specifications

Product Data
Description	GLI1 MS Standard C13 and N15-labeled recombinant protein (NP_005260)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC201110
Predicted MW	117.9 kDa
Protein Sequence	Sequence Link (showhide) RC201110
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_005260
RefSeq Size	3618
RefSeq ORF	3318
Synonyms	GLI; PAPA8; PPD1
Locus ID	2735
Cytogenetics	12q13.3
Summary	This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Protein Families	Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
Protein Pathways	Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer

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