MTHFS (NM_006441) Human Mass Spec Standard

Specifications

Product Data
Description	MTHFS MS Standard C13 and N15-labeled recombinant protein (NP_006432)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC205749
Predicted MW	23.3 kDa
Protein Sequence	Sequence Link (showhide) >RC205749 protein sequence Red=Cloning site Green=Tags(s) MAAAAVSSAKRSLRGELKQRLRAMSAEERLRQSRVLSQKVIAHSEYQKSKRISIFLSMQDEIETEEIIKD IFQRGKICFIPRYRFQSNHMDMVRIESPEEISLLPKTSWNIPQPGEGDVREEALSTGGLDLIFMPGLGFD KHGNRLGRGKGYYDAYLKRCLQHQEVKPYTLALAFKEQICLQVPVNENDMKVDEVLYEDSSTA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_006432
RefSeq Size	2346
RefSeq ORF	609
Synonyms	HsT19268; NEDMEHM
Locus ID	10588
Cytogenetics	15q25.1
Summary	The protein encoded by this gene is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, a precursor of reduced folates involved in 1-carbon metabolism. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
Protein Pathways	Metabolic pathways, One carbon pool by folate

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