PMF1 (NM_007221) Human Mass Spec Standard

Specifications

Product Data
Description	PMF1 MS Standard C13 and N15-labeled recombinant protein (NP_009152)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC209547
Predicted MW	23.3 kDa
Protein Sequence	Sequence Link (showhide) >RC209547 protein sequence Red=Cloning site Green=Tags(s) MAEASSANLGSGCEEKRHEGSSSESVPPGTTISRVKLLDTMVDTFLQKLVAAGSYQRFTDCYKCFYQLQP AMTQRIYDKFIAQLQTSIREEISDIKEEGNLEAVLNALDKIVEEGKVRKEPAWRPSGIPEKDLHSVIAPY FLQQRDTLRRHVQKQEAENQQLADAVLAGRRQVEELQLQVQAQQQAWQALHREQRELVAVLREPE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_009152
RefSeq Size	1122
RefSeq ORF	615
Locus ID	11243
Cytogenetics	1q22
Summary	Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.[UniProtKB/Swiss-Prot Function]
Protein Families	Stem cell - Pluripotency

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