Neurofilament L (68kDa)

Specifications

Product Data
Description	Neurofilament L (68kDa) bovine protein, 0.25 mg
Species	Bovine
Protein Source	Spinal Cord
Expression cDNA Clone or AA Sequence	sourced from bovine spinal cord
Predicted MW	68 kDa
Concentration	lot specific
Purity	>98% (determined by SDS gelelectrophoresis).
Buffer	Presentation State: Purified State: Lyophilized. Reconstitution: Restore with 200 µl distilled water (final volume 250 μl). Final solution: 10 mM Sodium Phosphate, pH 7.5, 2 mM DTT, 6M Urea, 10 mM Methylammonium chloride, 1 mM EDTA
Preparation	Lyophilized
Applications	Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization.
Protein Description	Standart Bovine Neurofilament 68 kD. Isoelectric Point: pI 5.0
Storage	Store at 2-8°C (lyophilized) or at -20°C (reconstituted). Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data
Summary	Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF L, NF M and NF H. NF L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Neurofilaments constitute the main structural elements of neuronal axons and dendrites. Neurofilaments are composed of three major subunits referred to as the neurofilament triplet, with molecular weights of 68kD, 160kD and 200kD. Neurofilament subunits are present in neurons, neuronal processes, peripheral nerves and sympathetic ganglion cells.

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