Collagen type IV Human Protein

Specifications

Product Data
Description	Collagen type IV human protein, 0.5 mg
Species	Human
Protein Source	Placenta
Concentration	lot specific
Purity	Chromatographically and Immunologically pure.
Buffer	State: Liquid (sterile filtered) purified protein Buffer System: 0.5M Acetic Acid Preservative: 0.01% Sodium Azide Stabilizer: None
Preparation	Liquid (sterile filtered) purified protein
Applications	ELISA. Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve. Immunoprecipitation. Western blotting.
Protein Description	This antigen is free from other Collagens, Human serum proteins and non-Collagen extracellular matrix proteins and reacts with anti-Collagen Type IV. Reaction with anti-Collagen I, II, III, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
Note	Caution: No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDCNIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Storage	Store vial at 2-8°C prior to opening. This product is stable 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
Stability	Shelf life: 6 months from despatch.
Reference Data
RefSeq	NP_001290039
Locus ID	1282
Cytogenetics	13q34
Synonyms	COL4A1
Summary	This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Protein Families	ELISA. Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve. Immunoprecipitation. Western blotting.
Protein Pathways	ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.