Casein Kinase 1 alpha (CSNK1A1) (NM_001892) Human Over-expression Lysate
CAT#: LC419689
CSNK1A1 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "Casein Kinase 1 alpha" proteins (4)
Need it in bulk or customized? Get a free quote |
CNY 900.00
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
Product Data | |
Description | CSNK1A1 HEK293T cell transient overexpression lysate (as WB positive control) |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC201797 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Predicted MW | 38.7 kDa |
Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reference Data | |
RefSeq | NP_001883 |
Locus ID | 1452 |
Cytogenetics | 5q32 |
Protein Families | WB |
Protein Pathways | Hedgehog signaling pathway, Wnt signaling pathway |
Documents
Product Manuals |
FAQs |
SDS |
Resources
蛋白相关资源 |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC422590 | CSNK1A1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
LY419689 | Transient overexpression lysate of casein kinase 1, alpha 1 (CSNK1A1), transcript variant 2 |
CNY 3,080.00 |
|
LY422590 | Transient overexpression lysate of casein kinase 1, alpha 1 (CSNK1A1), transcript variant 1 |
CNY 3,080.00 |
|
TP761718 | Purified recombinant protein of Human casein kinase 1, alpha 1 (CSNK1A1), transcript variant 1, full length, with N-terminal His tag, expressed in E. coli, 50ug |
CNY 2,040.00 |
其它Casein Kinase 1 alpha产品