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NLRP3 (NM_001079821) Human Over-expression Lysate
CAT＃: LC421550
NLRP3 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
View other "NLRP3" proteins (8)
|
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CNY 1,470.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
| Product Data | |
| Description | NLRP3 HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC213623 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 118 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_001073289 |
| Locus ID | 114548 |
| Cytogenetics | 1q44 |
| Protein Families | WB |
| Protein Pathways | NOD-like receptor signaling pathway |
Citations (2)
| The use of this Proteins has been cited in the following citations: |
|---|
|
Inflammasome-Independent Role of NLRP3 Mediates Mitochondrial Regulation in Renal Injury
,null,
Frontiers in Immunology
,PubMed ID 30483252
[NLRP3]
|
|
NLRP3 expression in mesencephalic neurons and characterization of a rare NLRP3 polymorphism associated with decreased risk of Parkinson’s disease
,null,
NPJ Parkinson's Disease
,PubMed ID 30131971
[NLRP3]
|
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC401526 | NLRP3 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 1,470.00 |
|
| LC426795 | NLRP3 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 1,470.00 |
|
| LY401526 | Transient overexpression lysate of NLR family, pyrin domain containing 3 (NLRP3), transcript variant 1 |
CNY 4,840.00 |
|
| LY421550 | Transient overexpression lysate of NLR family, pyrin domain containing 3 (NLRP3), transcript variant 3 |
CNY 4,840.00 |
|
| LY426795 | Transient overexpression lysate of NLR family, pyrin domain containing 3 (NLRP3), transcript variant 4 |
CNY 4,840.00 |
|
| TP320952 | Purified recombinant protein of Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 1, full length, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20 µg |
CNY 2,900.00 |
|
| TP750176 | Purified recombinant protein of Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 3, full length, with C-terminal HIS tag, expressed in E.Coli, 50 ug |
CNY 2,040.00 |
|
| TP762656 | Purified recombinant protein of Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 4 |
CNY 2,480.00 |
