KCNQ1 (NM_000218) Human Over-expression Lysate
CAT#: LC424989
KCNQ1 HEK293T cell transient overexpression lysate (as WB positive control)
View other "KCNQ1" proteins (5)
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CNY 1,470.00
Cited in 1 publication. |
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CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
Product Data | |
Description | KCNQ1 HEK293T cell transient overexpression lysate (as WB positive control) |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC219869 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Predicted MW | 74.5 kDa |
Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reference Data | |
RefSeq | NP_000209 |
Locus ID | 3784 |
Cytogenetics | 11p15.5-p15.4 |
Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
Protein Pathways | Vibrio cholerae infection |
Citations (1)
The use of this Proteins has been cited in the following citations: |
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Compound Heterozygous KCNQ1 Mutations Causing Recessive Romano–Ward Syndrome: Functional Characterization by Mutant Co-expression
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Frontiers in Cardiovascular Medicine
,PubMed ID 33693037
[KCNQ1]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC405589 | KCNQ1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 1,470.00 |
|
LC424853 | KCNQ1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 1,470.00 |
|
LY405589 | Transient overexpression lysate of potassium voltage-gated channel, KQT-like subfamily, member 1 (KCNQ1), transcript variant 2 |
CNY 4,840.00 |
|
LY424853 | Transient overexpression lysate of potassium voltage-gated channel, KQT-like subfamily, member 1 (KCNQ1), transcript variant 1 |
CNY 4,840.00 |
|
TP762235 | Purified recombinant protein of Human potassium voltage-gated channel, KQT-like subfamily, member 1 (KCNQ1), transcript variant 2, Ala257-End, with N-terminal His tag, expressed in E.coli, 50ug |
CNY 1,948.00 |