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CD272 (BTLA) (NM_001085357) Human Over-expression Lysate
CAT＃: LC425974
BTLA HEK293T cell transient overexpression lysate (as WB positive control)
View other "CD272" proteins (10)
|
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CNY 900.00
Product images
CNY 1,999.00
CNY 2,700.00
CNY 300.00
CNY 1,430.00
CNY 600.00
Specifications
| Product Data | |
| Description | BTLA HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC220628 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 27.1 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_001078826 |
| Locus ID | 151888 |
| Cytogenetics | 3q13.2 |
| Protein Families | Transmembrane |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC403628 | BTLA HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC421272 | BTLA HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY403628 | Transient overexpression lysate of B and T lymphocyte associated (BTLA), transcript variant 1 |
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| LY421272 | Transient overexpression lysate of B and T lymphocyte associated (BTLA), transcript variant 2 |
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| PH319458 | BTLA MS Standard C13 and N15-labeled recombinant protein (NP_861445) |
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| TP319458 | Recombinant protein of human B and T lymphocyte associated (BTLA), transcript variant 1, 20 µg |
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| TP700213 | Purified recombinant protein of Human B and T lymphocyte associated protein (BTLA), with C-terminal DDK/His tag, expressed in human cells |
CNY 6,650.00 |
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| TP700215 | Purified recombinant protein of Human B and T lymphocyte associated protein (BTLA), with C-terminal DDK/His tag, expressed in human cells |
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| TP720688 | Purified recombinant protein of Human B and T lymphocyte associated (BTLA), transcript variant 1 |
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| TP723966 | Human BTLA Protein, mFc-His Tag |
CNY 5,249.00 |
