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FAK (PTK2) (NM_005607) Human Over-expression Lysate
CAT＃: LC429259
PTK2 HEK293T cell transient overexpression lysate (as WB positive control)
Size: 100 ug
|
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CNY 1,470.00
| Cited in 1 publication. |
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Specifications
| Product Data | |
| Description | PTK2 HEK293T cell transient overexpression lysate (as WB positive control) |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC221398 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 121.5 kDa |
| Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reference Data | |
| RefSeq | NP_005598 |
| Locus ID | 5747 |
| Cytogenetics | 8q24.3 |
| Protein Families | WB |
| Protein Pathways | Axon guidance, Chemokine signaling pathway, ErbB signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer, VEGF signaling pathway |
Citations (1)
| The use of this Proteins has been cited in the following citations: |
|---|
|
Direct Detection of Protein Biomarkers in Human Fluids Using Site-Specific Antibody Immobilization Strategies
,null,
Sensors (Basel, Switzerland)
,PubMed ID 24481229
[PTK2]
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