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CD44 (NM_000610) Human Over-expression Lysate
CAT＃: LY400203
Transient overexpression lysate of CD44 molecule (Indian blood group) (CD44), transcript variant 1
Size: 20 ug
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CNY 4,840.00
| Cited in 1 publication. |
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Specifications
| Product Data | |
| Description | Transient overexpression lysate of CD44 molecule (Indian blood group) (CD44), transcript variant 1 |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC221771 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| Predicted MW | 81.54 kDa |
| Components | 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer 1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT) |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_000601 |
| Locus ID | 960 |
| Cytogenetics | 11p13 |
| Protein Families | WB |
| Protein Pathways | ECM-receptor interaction, Hematopoietic cell lineage |
Citations (1)
| The use of this Proteins has been cited in the following citations: |
|---|
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Anti-Peptide Monoclonal Antibodies Generated for Immuno-Multiple Reaction Monitoring-Mass Spectrometry Assays Have a High Probability of Supporting Western blot and ELISA
,Schoenherr, RM;Saul, RG;Whiteaker, JR;Yan, P;Whiteley, GR;Paulovich, AG;,
Mol. Cell Proteomics
,PubMed ID 25512614
[CD44]
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Other Versions
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| LC424334 | CD44 HEK293T cell transient overexpression lysate (as WB positive control) |
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| LY424332 | Transient overexpression lysate of CD44 molecule (Indian blood group) (CD44), transcript variant 2 |
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| PH302455 | CD44 MS Standard C13 and N15-labeled recombinant protein (NP_001001389) |
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| PH321771 | CD44 MS Standard C13 and N15-labeled recombinant protein (NP_000601) |
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| TP302455 | Recombinant protein of human CD44 molecule (Indian blood group) (CD44), transcript variant 2, 20 µg |
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| TP321771 | Recombinant protein of human CD44 molecule (Indian blood group) (CD44), transcript variant 1, 20 µg |
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| TP720465 | Recombinant protein of human CD44 molecule (Indian blood group) (CD44), transcript variant 1 |
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CD44 mouse monoclonal antibody, clone OTI1D8 (formerly 1D8)
