SERPINE1 / PAI1 (24-402, His-tag) Human Protein
CAT#: AR09064PU-N
SERPINE1 / PAI1 (24-402, His-tag) human recombinant protein, 0.1 mg
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CNY 8,900.00
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规格
Specifications
Product Data | |
Species | Human |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
MGSSHHHHHH SSGLVPRGSH MVHHPPSYVA HLASDFGVRV FQQVAQASKD RNVVFSPYGV ASVLAMLQLT TGGETQQQIQ AAMGFKIDDK GMAPALRHLY KELMGPWNKD EISTTDAIFV QRDLKLVQGF MPHFFRLFRS TVKQVDFSEV ERARFIINDW VKTHTKGMIS NLLGKGAVDQ LTRLVLVNAL YFNGQWKTPF PDSSTHRRLF HKSDGSTVSV PMMAQTNKFN YTEFTTPDGH YYDILELPYH GDTLSMFIAA PYEKEVPLSA LTNILSAQLI SHWKGNMTRL PRLLVLPKFS LETEVDLRKP LENLGMTDMF RQFQADFTSL SDQEPLHVAQ ALQKVKIEVN ESGTVASSST AVIVSARMAP EEIIMDRPFL FVVRHNPTGT VLFMGQVMEP
|
Tag | His-tag |
Predicted MW | 45 kDa |
Concentration | lot specific |
Purity | ≥95 by SDS PAGE |
Buffer | Presentation State: Purified State: Liquid purified protein Buffer System: 150 mM Na2HPO4, pH 6.6, 0.5 mM NaCl, 2 mM Glutathione, 0.01% Tween-80 |
Preparation | Liquid purified protein |
Protein Description | Recombinant human PAI-1, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Bioactivity | Specific: The IC50 value is < 56 nM. Measured by its ability to inhibit uPA cleavage of the substrate Z - GGR - AMC. Activity Assay: 1. Prepare a recombinant PAI - 1 with various concentrations (2 , 000 nM, 1 , 000 nM, 500 nM, 250 nM, 125 nM) in assay buffer. ( Assay buffer: 50 mM Tris - HCl pH 8.0, 0 .01% Tween 20) 2. Mix equal volumes of the PAI - 1 protein and diluted 2 ug/ml uPA (Prospec . Cat No. ENZ - 264) 3. Incubate at room temperature for 15 minutes. 4 . Dilute incubated reaction mixtures (PAI - 1, uPA) by a 5 fold dilution in assay buffer, mix 50ul of 200uM substrate (Z - GGR - AMC) and diluted mixtures. 5 . Read excitation wavelength 355 nm and emission wavelength 460nm for 10 minutes. - Z - GGR - AMC (CALBIOCHEM, Cat.No, 672159) - 96 well Polystyrene Microplate, black (greniner bio - one, Cat.No , 655077) - Fluorescent plate reader (PerkinElmer, VICTORX3) |
Reference Data | |
RefSeq | NP_000593 |
Locus ID | 5054 |
UniProt ID | P05121, A0A024QYT5 |
Cytogenetics | 7q22.1 |
Synonyms | PAI; PAI-1; PAI1; PLANH1 |
Summary | This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. The protein also functions as a component of innate antiviral immunity. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. [provided by RefSeq, Aug 2020] |
Protein Families | Druggable Genome, Secreted Protein |
Protein Pathways | Complement and coagulation cascades, p53 signaling pathway |
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