Recombinant Proteins

Proteins

Recombinant Proteins

Mutant Proteins

Human

Mouse

SARS-CoV-2

Cynomolgus

Rat

Escherichia coli

Rhesus

Blomia tropicalis

Bovine

2019-nCoV

Dermatophagoides pteronyssinus

Orf Virus

Rabbit

Staphylococcus aureus

Streptococcus Pyogenes

Hepatitis B Virus

Saccharomyces cerevisiae

Cavia porcellus

Chicken

Dermatophagoides farinae

HIV

Streptococcus sp.

Alternaria alternata

B.subtilis

Betula pendula

Campylobacter jejuni

Carassiusauratus

Chlorocebus aethiops

D. aromatica

Drosophila melanogaster

E.coli

Enterobacteria phage T4

Escherichia phage T7

Hirudo medicinalis

Human herpesvirus

Human papillomavirus

Humon

Humulus japonicus

Klebsiella pneumoniae

Lachnospiraceae bacterium ND2006

Lepidoglyphus destructor

Leptotrichia wadei

Macaca fascicularis

Marmoset

Mucor hiemalis

Mus musculus

Pig

Proteobacteria

S. hygroscopicus

SIV

Serratia marcescens

Sheep

Staphylococcus aureus subsp.

Streptococcus

Streptomyces avidinii

Streptomyces plicatus

T. fusca

TEV

Tobacco etch virus

Vaccinia virus

Vacciniavirus

Vibrio cholerae

viral

VEGFA

ALPHA 1 ANTITRYPSIN

NCALD

FGF1

S PROTEIN

IRF5

NDRG2

FGF2

FGFR1

ISOCITRATE DEHYDROGENASE

ALKALINE PHOSPHATASE

ALPHA SYNUCLEIN

CPNE1

IL6

RIBONUCLEASE INHIBITOR

MORTALITY FACTOR 4 LIKE 2

PD-L1

PQBP1

TNF ALPHA

UBE2D3

HMGA1

IGF1

IL2

M-CSF

MAX

MTH1

PRKAR1B

TPM1

AURORA A

EGFR

FGL1

FSTL1

ICAM2

IL22

IL4

MYOSIN

FHL1

PCBP2

PGF

S100 CALCIUM BINDING PROTEIN A13

SULT1A1

UBE2I

ALDOLASE

B3GALT5

BETA 2 MICROGLOBULIN

CATHEPSIN B

CD36

CD3E

CD47

CTLA4

GLUTAMINE SYNTHETASE

HER2

HMG1

IL33

IL8

LIF

MBNL1

MYELIN BASIC PROTEIN

NDUF3

PCSK9

TROPOMYOSIN 3

14-3-3 ZETA

ACRV1

ASPARTATE BETA HYDROXYLASE

CEACAM1

CSF2

ERBB 3

GALE

GM CSF

HNF 4 ALPHA

HNRNP F

IL12B

IL1RA

P38

ROR1

TIE1

ANNEXIN A2

ARSA

CARBONIC ANHYDRASE X

CD10

CD147

CD83

CUTA

DYNLL1

ESTROGEN RELATED RECEPTOR GAMMA

FHL2

FKBP2

ICAM1

IDH2

IL18

LAMP2

LIVER CARBOXYLESTERASE 1

MICA

OSTEOPONTIN

PD1

PTPRN

S100A8

SIRP ALPHA

TAU

TIGIT

HEK293T

E. coli

HEK293

Sf9

Insect

CHO

Baculovirus

Pichia

Pichia pastoris

Mouse

Other

S. cerevisiae

Sf9 insect

human serum

H. vulgare

Human

Yeast

C-Myc/DDK

C-MYC/DDK

C-His

N-His

Tag Free

N-GST and C-His

C-Fc

C-6His

C-Human Fc

C-DDK

C-6×His

N-GST and C-HIS

N-GST

C-DDK/His

His-tag

Myc-DDK

C-Fc&His

C-FLAG

N-6His

C-Mouse Fc

N-His-ABP (Albumin-Binding Protein)

N-Human Fc

C-mFc

C-HIS

N-His-SUMO

His-Tag

N-His&C-His

C-6His-Avi

C-8His

C-Mouse Fc and 6×His

C-hFc

N-T7&C-His

N-Trx&His

C-Avi-6His

N-His-Trx

C-Fc-Avi

C-Human Fc and 6×His

C-10His

N-Fc

N-GST, C-His

hIgG-His-Tag

N-HIS

N-His&SUMO

C- 6×His

N-6×His

N-GST&His

Tag-free

C-Avi&6His

C-6xHis

hIgG-Tag

C-AVI&His

C-Fc&AVI

N-8His

N-Avi

N-His PDCD1(Pro21-Val170)

N-His/DDK

N-Mouse Fc and C-6×His

C-6×His tag and C-Human Fc

C-TEV-8His

C-mouse Fc

N-6His-Avi

N-His&C-Fc

N-His&T7

N-His-GST

N-MARI

N-Trx

C-Fc/His

C-Flag

C-hFc&His

His-tagged, myc-tagged

N-DDK

N-His&Flag

N-mFc

C-3×Flag Tag

C-6xHis/AVI

C-7×His

C-Avi&His

C-DDK-His

C-FC

C-Fc-Flag&C-Fc-6His

C-HUMAN

N-6His-Flag

N-8His&N-FLAG

N-FC

N-FLAG

N-Fc-FLAG

N-His-PDCD1(Pro21-Val170)

N-Mouse Fc

N-terminal His

N-terminal His-PDCD1(Pro21-Val170) tag

C-AVI

C-AVI-PLUS

C-Avi

C-Avi-10His

C-Avi-His

C-DDK/10xHis

C-DDK/6xHis

C-FC-6HIS&C-FC-Flag

C-FC/6xHis

C-FLAG-6His

C-FLAG-Fc

C-Fc Human

C-Fc Mouse

C-Flag,C-6His

C-GST

C-HIS/C

C-HIS/DDK

C-His&AVI

C-His&Flag

C-His&HA&AVI

C-His/AVIplus

C-His/DDK

C-Human Fc, Avi

C-Human Fc-Avi

C-MIgG2a

C-hIgG1 Fc

C-hIgG2 Fc

C-hIgG4 Fc

C-hIgG4P Fc

C-mFC

C-mFc&His

C-mIgG2AFc

C-mIgG2a Fc

C-mIgG2aFc

C-terminal DDK

C-terminal His

DDK-tag

His tag

N-10*His

N-10His

N-10His-Flag

N-6His&Avi

N-6His, C-6His

N-6His-FLAG

N-8His-Flag

N-AVI

N-AVI&His

N-Avi&6His

N-BBP&His

N-DDK/His

N-FUSION

N-Flag&C-His

N-Flag&His

N-GFP

N-GST(CKM) and N-His(CKB)

N-HIS and C-AVIPLUS

N-His&MBP

N-PROTEIN

N-T7

N-TRX

N-TRXA

N-Trx-His

N-hFc

N-terminal His-tag

N-terminal murine Fc Tag

No

Tag free

PE

FITC

HRP

Yes

MVPro

SDS PAGE

Druggable Genome

Transmembrane

Secreted Protein

Transcription Factors

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

Cancer stem cells

P450

GPCR

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Potassium

Ion Channels: Glutamate Receptors

Ion Channels: Cys-loop Receptors

the solution can be further dialyzed against PBS (phosphate buffered saline

Ion Channels: ATP Receptors

Ion Channels: Transient receptor potential

10 mM Tris-HCl

2 mM dithiothreitol

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Ion Channels: Sodium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cyclic nucleotide gated

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

ELISA.

ELISA.
Western blotting.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/40

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western blot: 1/10

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Western blotting.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA

ELISA.
Functional Assays.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

Metabolic pathways

Pathways in cancer

Cytokine-cytokine receptor interaction

MAPK signaling pathway

Focal adhesion

Regulation of actin cytoskeleton

Jak-STAT signaling pathway

Chemokine signaling pathway

Endocytosis

Natural killer cell mediated cytotoxicity

Neurotrophin signaling pathway

Hematopoietic cell lineage

T cell receptor signaling pathway

Toll-like receptor signaling pathway

Cell adhesion molecules (CAMs)

Ubiquitin mediated proteolysis

Purine metabolism

Insulin signaling pathway

Alzheimer's disease

Axon guidance

Wnt signaling pathway

Cell cycle

Apoptosis

ErbB signaling pathway

Leukocyte transendothelial migration

Prostate cancer

Huntington's disease

Melanoma

Pancreatic cancer

Tight junction

Progesterone-mediated oocyte maturation

Adherens junction

Fc epsilon RI signaling pathway

Oocyte meiosis

Colorectal cancer

GnRH signaling pathway

Calcium signaling pathway

Lysosome

Spliceosome

Pyrimidine metabolism

TGF-beta signaling pathway

Renal cell carcinoma

Hypertrophic cardiomyopathy (HCM)

Fc gamma R-mediated phagocytosis

NOD-like receptor signaling pathway

RIG-I-like receptor signaling pathway

Dilated cardiomyopathy

Chronic myeloid leukemia

Glioma

B cell receptor signaling pathway

Systemic lupus erythematosus

VEGF signaling pathway

Small cell lung cancer

Antigen processing and presentation

Epithelial cell signaling in Helicobacter pylori infection

PPAR signaling pathway

Parkinson's disease

Adipocytokine signaling pathway

Glycolysis / Gluconeogenesis

p53 signaling pathway

Gap junction

Bladder cancer

Complement and coagulation cascades

Acute myeloid leukemia

Cytosolic DNA-sensing pathway

Melanogenesis

Vascular smooth muscle contraction

Endometrial cancer

Non-small cell lung cancer

Pathogenic Escherichia coli infection

Long-term potentiation

Phosphatidylinositol signaling system

Arginine and proline metabolism

Type I diabetes mellitus

Amyotrophic lateral sclerosis (ALS)

Autoimmune thyroid disease

Oxidative phosphorylation

Long-term depression

Viral myocarditis

mTOR signaling pathway

ECM-receptor interaction

Metabolism of xenobiotics by cytochrome P450

Drug metabolism - cytochrome P450

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Glutathione metabolism

Amino sugar and nucleotide sugar metabolism

Allograft rejection

Inositol phosphate metabolism

Cardiac muscle contraction

Neuroactive ligand-receptor interaction

Type II diabetes mellitus

Graft-versus-host disease

Glycerophospholipid metabolism

Tyrosine metabolism

Fructose and mannose metabolism

Thyroid cancer

Drug metabolism - other enzymes

Retinol metabolism

Valine

Vibrio cholerae infection

Prion diseases

Pyruvate metabolism

Tryptophan metabolism

Alanine

Starch and sucrose metabolism

aspartate and glutamate metabolism

Proteasome

Ribosome

Regulation of autophagy

leucine and isoleucine degradation

Cysteine and methionine metabolism

Primary immunodeficiency

RNA degradation

Hedgehog signaling pathway

Arachidonic acid metabolism

Glycerolipid metabolism

Base excision repair

Fatty acid metabolism

Nitrogen metabolism

Androgen and estrogen metabolism

Nucleotide excision repair

Porphyrin and chlorophyll metabolism

Pentose phosphate pathway

Asthma

Citrate cycle (TCA cycle)

Galactose metabolism

Butanoate metabolism

DNA replication

Propanoate metabolism

Basal cell carcinoma

Lysine degradation

Sphingolipid metabolism

Histidine metabolism

Olfactory transduction

Aminoacyl-tRNA biosynthesis

Notch signaling pathway

beta-Alanine metabolism

SNARE interactions in vesicular transport

Nicotinate and nicotinamide metabolism

Glycine

serine and threonine metabolism

Ether lipid metabolism

Dorso-ventral axis formation

Maturity onset diabetes of the young

Phenylalanine metabolism

Selenoamino acid metabolism

Sulfur metabolism

Linoleic acid metabolism

Mismatch repair

Renin-angiotensin system

Glyoxylate and dicarboxylate metabolism

Riboflavin metabolism

Pantothenate and CoA biosynthesis

Pentose and glucuronate interconversions

RNA polymerase

Glycosaminoglycan degradation

Homologous recombination

Other glycan degradation

Basal transcription factors

Terpenoid backbone biosynthesis

N-Glycan biosynthesis

Ascorbate and aldarate metabolism

One carbon pool by folate

Glycosphingolipid biosynthesis - lacto and neolacto series

alpha-Linolenic acid metabolism

Primary bile acid biosynthesis

Taste transduction

Folate biosynthesis

C21-Steroid hormone metabolism

O-Glycan biosynthesis

Glycosphingolipid biosynthesis - globo series

Limonene and pinene degradation

Thiamine metabolism

Keratan sulfate biosynthesis

Synthesis and degradation of ketone bodies

ABC transporters

Biosynthesis of unsaturated fatty acids

Circadian rhythm - mammal

Heparan sulfate biosynthesis

Methane metabolism

Non-homologous end-joining

Steroid biosynthesis

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Ubiquinone and other terpenoid-quinone biosynthesis

Chondroitin sulfate biosynthesis

Taurine and hypotaurine metabolism

Glycosphingolipid biosynthesis - ganglio series

Phenylalanine

leucine and isoleucine biosynthesis

tyrosine and tryptophan biosynthesis

Caffeine metabolism

Fatty acid elongation in mitochondria

Protein export

Fatty acid biosynthesis

Lysine biosynthesis

Vitamin B6 metabolism

D-Glutamine and D-glutamate metabolism

Cyanoamino acid metabolism

Biotin metabolism

D-Arginine and D-ornithine metabolism

O-Mannosyl glycan biosynthesis

Lipoic acid metabolism

E. coli

Human

Human 293

P.Pichia

Insect

Baculovirus

HEK293

Pichia pastoris

293F

CHO Stable

Expi293

Mammalian

1

2

3

Yes

In Stock